본문 바로가기
KERD
논문KCI 등재

Conjugated linoleic acid producing potential of lactobacilli isolated from goat (AXB) rumen fluid samples

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

초록

Objective: The present investigation was aimed to explore the potential of lactobacilli for conjugated linoleic acid (CLA) production, isolated from rumen fluid samples of lactating goats. Methods: A total of 64 isolates of lactobacilli were obtained using deMan-Rogosa-Sharpe (MRS) agar from rumen fluid of goats and further subjected to morphological and biochemical characterizations. Isolates found as gram-positive, catalase negative rods were presumptively identified as Lactobacillus species and further confirmed by genus specific polymerase chain reaction (PCR). The phylogenetic tree was constructed from the nucleotide sequences using MEGA6. Results: Out of the 64 isolates, 23 isolates were observed positive for CLA production by linoleate isomerase gene-based amplification and quantitatively by UV-spectrophotometric assay for the conversion of linoleic acid to CLA as well as gas chromatography-based assay. In all Lactobacillus species cis9, trans11 isomer was observed as the most predominant CLA isomer. These positive isolates were identified by 16S rRNA gene-based PCR sequencing and identified to be different species of L. ingluviei (2), L.salivarius (2), L. curvatus (15), and L. sakei (4). Conclusion: The findings of the present study concluded that lactic acid bacteria isolated from ruminal fluid samples of goat have the potential to produce bioactive CLA and may be applied as a direct fed microbial to enhance the nutraceutical value of animal food products.

Abstract

Objective: The present investigation was aimed to explore the potential of lactobacilli for conjugated linoleic acid (CLA) production, isolated from rumen fluid samples of lactating goats. Methods: A total of 64 isolates of lactobacilli were obtained using deMan-Rogosa-Sharpe (MRS) agar from rumen fluid of goats and further subjected to morphological and biochemical characterizations. Isolates found as gram-positive, catalase negative rods were presumptively identified as Lactobacillus species and further confirmed by genus specific polymerase chain reaction (PCR). The phylogenetic tree was constructed from the nucleotide sequences using MEGA6. Results: Out of the 64 isolates, 23 isolates were observed positive for CLA production by linoleate isomerase gene-based amplification and quantitatively by UV-spectrophotometric assay for the conversion of linoleic acid to CLA as well as gas chromatography-based assay. In all Lactobacillus species cis9, trans11 isomer was observed as the most predominant CLA isomer. These positive isolates were identified by 16S rRNA gene-based PCR sequencing and identified to be different species of L. ingluviei (2), L.salivarius (2), L. curvatus (15), and L. sakei (4). Conclusion: The findings of the present study concluded that lactic acid bacteria isolated from ruminal fluid samples of goat have the potential to produce bioactive CLA and may be applied as a direct fed microbial to enhance the nutraceutical value of animal food products.

참고문헌 (36)

  1. German JB, "A reappraisal of the impact of dairy foods and milk fat on cardiovascular disease risk", Eur J Nutr, 48, pp.191~203 (2009)[DOI]
  2. Kim L, "trans-10,cis-12 CLA promotes osteoblastogenesis via SMAD mediated mechanism in bone marrow mesenchymal stem cells", J Funct Foods, 8, pp.367~376 (2014)[DOI]
  3. Yang B, "Synthesis of conjugated linoleic acid by the linoleate isomerase complex in food-derived lactobacilli", J Appl Microbiol, 117, pp.430~439 (2014)[DOI]
  4. Yang B, "Review of the roles of conjugated linoleic acid in health and disease", J Funct Food, 15, pp.314~325 (2015)[DOI]
  5. Kepler CR, "Intermediates and products of the biohydrogenation of linoleic acid by Butyrinvibrio Fibrisolvens", J Biol Chem, 241, pp.1350~1354 (1966)
  6. Corl BA, "The role of Δ9-desaturase in the production of cis-9, trans-11 CLA", J Nutr Biochem, 12, pp.622~630 (2001)[DOI]
  7. Benjamin S, "Pros and cons of CLA consumption : an insight from clinical evidences", Nutr Metab, 12, pp.4~ (2015)[DOI]
  8. Macdonald HB, "Conjugated linoleic acid and disease prevention : a review of current knowledge", J Am Coll Nutr, 19, pp.111S~118S (2000)[DOI]
  9. Sosa-Castañeda J, "Screening of Lactobacillus strains for their ability to produce conjugated linoleic acid in milk and to adhere to the intestinal tract", J Dairy Sci, 98, pp.6651~6659 (2015)[DOI]
  10. Griinari JM, "Advances in conjugated linoleic acid research", pp.180~201 (1999), AOCS Press
  11. Puniya AK, "Conjugated linoleic acid producing potential of lactobacilli isolated from the rumen of cattle", J Ind Microbiol Biotechnol, 35, pp.1223~1228 (2008)[DOI]
  12. Asraf Hussain SK, "Characterization of CLA-producing Butyrivibrio spp. reveals strain-specific variations", 3 Biotech, 6, pp.90~ (2016)[DOI]
  13. Dahiya DK, "Isolation, molecular characterization and screening of indigenous lactobacilli for their abilities to produce bioactive conjugated linoleic acid(CLA)", J Food Sci Technol, 54, pp.792~801 (2017)[DOI]
  14. Jena R, "Isolation and species delineation of genus Bifidobacterium using PCR-RFLP of partial hsp60 gene fragment", LWT-Food Sci Technol, 80, pp.286~293 (2017)[DOI]
  15. Dubernet S, "A PCR-based method for identification of lactobacilli at the genus level", FEMS Microbiol Lett, 214, pp.271~275 (2002)[DOI]
  16. Gorissen L, "Linoleate isomerase activity occurs in lactic acid bacteria strains and is affected by pH and temperature", J Appl Microbiol, 111, pp.593~606 (2011)[DOI]
  17. Barrett E, "Rapid screening method for analyzing the conjugated linoleic acid production capabilities of bacterial cultures", Appl Environ Microbiol, 73, pp.2333~2337 (2007)[DOI]
  18. O’Fallon JV, "A direct method for fatty acid methyl ester synthesis : application to wet meat tissues, oils, and feedstuffs", J Anim Sci, 85, pp.1511~1521 (2007)[DOI]
  19. Lane DJ, "Nucleic acid techniques inbacterial systematics", pp.115~176 (1991), John Wiley
  20. Ogawa J, "Conjugated linoleic acid accumulation via 10-hydroxy-12-octadecaenoic acid during microaerobic transformation of linoleic acid by Lactobacillus acidophilus", Appl Environ Microbiol, 67, pp.1246~1252 (2001)[DOI]
  21. Khosravi A, "Bioconversion enhancement of conjugated linoleic acid by Lactobacillus plantarum using the culture media manipulation and numerical optimization", J Food Sci Technol, 52, pp.5781~5789 (2015)[DOI]
  22. Kishino S, "Conjugated linoleic acid production from linoleic acid by lactic acid bacteria", J Am Oil Chem Soc, 79, pp.159~163 (2002)[DOI]
  23. Andrade JC, "Production of conjugated linoleic acid by food‐grade bacteria : a review", Int J Dairy Technol, 65, pp.467~481 (2012)[DOI]
  24. Li H, "Conjugated linoleic acid conversion by six Lactobacillus plantarum strains cultured in MRS broth supplemented with sunflower oil and soymilk", J Food Sci, 77, pp.M330~6 (2012)[DOI]
  25. Lee K, "Antiobesity effect of trans-10, cis-12-conjugated linoleic acid-producing Lactobacillus plantarum PL62 on diet-induced obese mice", J Appl Microbiol, 103, pp.1140~1146 (2007)[DOI]
  26. Lin TY, "Production of conjugated linoleic acid by enzyme extract of Lactobacillus acidophilus CCRC 14079", Food Chem, 83, pp.27~31 (2003)[DOI]
  27. Ando A, "CLA production from ricinoleic acid by lactic acid bacteria", J Am Oil Chem Soc, 80, pp.889~894 (2003)[DOI]
  28. Farmani J, "Conjugated linoleic acid-producing enzymes : a bioinformatics study", Eur J Lipid Sci Technol, 112, pp.1088~1100 (2010)[DOI]
  29. Koort J, "Lactobacillus curvatus subsp. melibiosus is a later synonym of Lactobacillus sakei subsp. carnosus", Int J Syst Evol Microbiol, 54, pp.1621~1626 (2004)[DOI]
  30. Lee J, "Identification of Lactobacillus sakei and Lactobacillus curvatus by multiplex PCR-based restriction enzyme analysis", J Microbiol Methods, 59, pp.1~6 (2004)[DOI]
  31. Klein G, "Emended descriptions of Lactobacillus sake (Katagiri, Kitahara, and Fukami) and Lactobacillus curvatus (Abo-Elnaga and Kandler): numerical classification revealed by protein fingerprinting and identification based on biochemical patterns and DNA-DNA hybridizations", Int J Syst Bacteriol, 46, pp.367~376 (1996)[DOI]
  32. Gorissen L, "Bacterial production of conjugated linoleic and linolenic acid in foods : a technological challenge", Crit Rev Food Sci Nutr, 55, pp.1561~1574 (2015)[DOI]
  33. 서자겸, "Direct-fed Microbials for Ruminant Animals", Animal Bioscience, 23(12), pp.1657~1667 (2010), 아세아·태평양축산학회
  34. Frizzo LS, "Lactic acid bacteria to improve growth performance in young calves fed milk replacer and spray-dried whey powder", Anim Feed Sci Technol, 157, pp.159~167 (2010)[DOI]
  35. Ting YS, "Characterization of conjugated linoleic acid-producing lactic acid bacteria as potential probiotic for chicken", Malays J Microbiol, 12, pp.15~23 (2016)[DOI]
  36. Mandal A, "Effect of newly isolated Lactobacillus ingluviei ADK10, from chicken intestinal tract on acetaminophen induced oxidative stress in Wistar rats", Indian J Exp Biol, 51, pp.174~180 (2013)

관련 논문AI추천

Efficient conversion of conjugated linoleic acid c9,t11 by Lactobacillus fermentation from vegetable oil to generate fermented milk with high CLA content양희선, 허창기한국식품저장유통학회 · Food Science and Preservation2018 · 제25권 제4호The Effect of Forage Level and Oil Supplement on Butyrivibrio fibrisolvens and Anaerovibrio lipolytica in Continuous Culture FermentersA.A. AbuGhazaleh, A. Ishlak, P. Gudla아세아·태평양축산학회 · Animal Bioscience2012 · 제25권 제2호Isolation and Identification of Noble Lactic Acid Bacteria유민, 민병태, Han-Cheol Yeo, Hyeong-Jun Park, Jin-Young Jang자연과학연구소 · Quantitative Bio-Science2018 · 제37권 제2호Effects of Lactation Stage and Individual Performance on Milk cis-9, trans-11 Conjugated Linoleic Acids Content in Dairy Cows이홍구, 최윤재, H.S. Kang, J.E. Hong, J. H. Kim, J.H. Kim, J. J. Oh, J.N. Lim, T. Wang아세아·태평양축산학회 · Animal Bioscience2013 · 제26권 제2호Probiotic Potential of Pediococcus acidilactici and Enterococcus faecium Isolated from Indigenous Yogurt and Raw Goat MilkIqbal Kabir Jahid, Md. Anwar Hossain, Md. Iqbal Hossain, Pravas Chandra Roy, Santonu Kumar Sanyal, Sharmin Akter Monika, Shovon Lal Sarkar한국미생물·생명공학회 · 한국미생물·생명공학회지2020 · 제48권 제3호The Dose-response Effects of Nano-encapsulated Conjugated Linoleic Acid on in vitro Ruminal Fermentation Characteristics허완, 김상범, 김영준, 김은태, 문여황, 박범영, 박지후, 안영대, 윤호백, 이성실, 정하연농업생명과학연구원 · 농업생명과학연구2017 · 제51권 제4호

이 간행물의 다른 논문 (5건)