Simple Purification of Adeno-Associated Virus-DJ for Liver-Specific Gene Expression
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Recombinant gene expression using adeno-associated viruses (AAVs) has become a valuable tool in animal studies, as they mediatesafe expression of transduced genes for several months. The liver is a major organ of metabolism, and liver-specific expressionof a gene can be an invaluable tool for metabolic studies. AAV-DJ is a recombinant AAV generated by the gene shuffling of various AAV serotypes and shares characteristics of AAV2 and AAV8. AAV-DJ contains a heparin-binding domain in its capsid, which suggests that a heparin column could be used for the purification of the AAV. Given that AAV-DJ has been only recently available, relatively little is known about the optimal preparation/purification and application of AAV-DJ. Here, we present a simplelarge-scale preparation method that can generate 3×1013 viral particles for in vivo experiments and demonstrate liver-specific gene expression via systemic injection in mice.
Abstract
Recombinant gene expression using adeno-associated viruses (AAVs) has become a valuable tool in animal studies, as they mediatesafe expression of transduced genes for several months. The liver is a major organ of metabolism, and liver-specific expressionof a gene can be an invaluable tool for metabolic studies. AAV-DJ is a recombinant AAV generated by the gene shuffling of various AAV serotypes and shares characteristics of AAV2 and AAV8. AAV-DJ contains a heparin-binding domain in its capsid, which suggests that a heparin column could be used for the purification of the AAV. Given that AAV-DJ has been only recently available, relatively little is known about the optimal preparation/purification and application of AAV-DJ. Here, we present a simplelarge-scale preparation method that can generate 3×1013 viral particles for in vivo experiments and demonstrate liver-specific gene expression via systemic injection in mice.
참고문헌 (20)
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