콩 종실의 조단백, 조지방, 지방산 분석을 위한 근적외선 분광분석기(NIRS) 검량식 작성과 재래종 자원 평가
Development of NIRS Equations and Mass Evaluation of Crude Protein, Oil and Composition of Fatty Acid by Near Infrared Reflectance Spectroscopy (NIRS) in Soybean Landraces from Korea
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초록
본 연구는 콩 유전자원의 조단백, 조지방과 지방산을 대량평가하기 위하여 NIRS 검량식을 작성하고 작성된 검량식으로국내 재래종 콩 854자원의 성분을 분석하기 위하여 수행하였다. 검량식 작성에 사용된 국내외 육성종의 실험실 분석값은 조단백 평균 38.8%, 범위 32.3~46.0%, 조지방 평균 15.0%, 범위11.1-23.7%로 조사되었으며, 지방산 조성의 평균 함량과 범위는각각 palmitic acid 11.2%, 9.2-14.7%, stearic acid 3.6%, 2.4-4.9%, oleic acid 23.7%, 15.0-39.0%, linoleic acid 54.5%, 41.4-61.2%, linolenic acid 7.0%, 4.1-11.1% 였다. 검량식의통계 분석 결과 R2 값은 각각 조단백 0.983, 조지방 0.969, palmitic acid 0.592, stearic acid 0.514, oleic acid 0.978, linoleic acid 0.961, linolenic acid 0.957 이었다. 검량식은 통계분석값에 따라 조단백, 조지방과 불포화 지방산 분석(oleic, linoleic, linolenic acid)에는 적용이 가능하였으나 포화 지방산(palmitic, stearic acid)에는 적용이 불가능하였다. 따라서 국내재래종 콩 854자원의 성분분석에 조단백, 조지방과 불포화 지방산의 검량식을 적용하였으며 그 결과 조단백 평균은 39.2%, 범위 33.7-47.0%, 조지방 평균 15.0%, 범위 9.8-20.3%로 조사되었다. 조단백 함량이 높은 2자원, IT154552(45.2%)와IT023955(47.0%)의 값을 재확인하기 위하여 정량 분석하였으며, 각각 45.1%와 46.9%로 조사되어, 두 방법간 함량 차이가없음을 알 수 있었다. 따라서, 본 연구결과의 단백질 검량식은대량평가에 매우 유용할 것으로 보인다. Oleic acid는 평균21.4%, 12.1-30.2%, linoleic acid 평균 55.6%, 47.8-62.3%, linolenic acid 평균 8.1%, 5.9-10.7%로 조사되었다. 조단백, 조지방, oleic acid, linoleic acid, linolenic acid을 이용하여상관관계를 조사한 결과, 조단백과 조지방은 부의 상관(-0.203), 조지방과 oleic acid는 강한 정의 상관(0.374), linolenic acid와는 강한 부의 상관(-0.666)이 있었다.
Abstract
This study was investigated to develop mass evaluation system for the contents of crude protein, oil and fatty acid in soybean germplasm using NIRS. NIRS equations were created with 345 soybeans, multiple correlation coefficients of crude protein, oil, palmitic, stearic, oleic, linoleic and linolenic acid between data obtained from NIRS and quantitative analysis were 0.983, 0.969, 0.592, 0.514, 0.978, 0.961 and 0.957, respectively. Equation statistics indicated that contents of crude protein, oil and unsaturated fatty acid except palmitic and stearic acid in soybean seed were suitable for determination by NIRS. Those NIRS equations were applied to examine crude protein, oil and unsaturated fatty acid of 854 soybean landraces from Korea. The average contents and ranges of crude protein and oil were 39.2% with a range of 33.7-47.0% and 15.0% with a range of 9.8-20.3%, individually. In addition, those of oleic, linoleic and linolenic acid were 21.4% with a range of 12.1-30.2%, 55.6% with a 47.8-62.3% and 8.1% with a range of 5.9-10.7% respectively. We conducted quantitative analysis to reconfirm with IT154552 (45.1%) and IT023955(46.9%) above 45% of crude protein, the results were similar from NIRS (45.2%, 47.0%). NIRS data for protein from this study made no difference with lab data, which would be useful for mass evaluation. There was negative correlation (-0.203) between crude protein and oil, positive correlation (0.379) between crude oil and oleic acid, and significantly negative correlation (-0.879) between oleic and linoleic acid.
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