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韓茵蔯, 鬱金, 山査, 丹蔘 발효복합추출물이 RAW264.7 및 HUVEC 세포에서 나타나는 이상지질혈증 관련 염증인자 발현 및 항산화에 미치는 영향

The Effects of an Extract of Fermented Artemisiae Iwayomogii Herba, Curcumae Longae, Crataegi Fructus and Salviae Miltiorrhizae Radix on Anti-inflammation Associated with Dyslipidemia and Anti-oxidation in RAW264.7 and HUVEC Cells

유호룡|김윤식|설인찬|이강욱|조현경
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2018.09|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제39권 제4호|pp. 480-494

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참고문헌

키워드

fermentationArtemisiae Iwayomogii HerbaCurcumae LongaeCrataegi FructusSalviae Miltiorrhizae Radix
주제분류한의학

초록

Objectives: To investigate the effect of fermented extract of Artemisiae Iwayomogii Herba, Curcumae Longae, Crataegi Fructus, and Salviae Miltiorrhizae Radix (FMH) on anti-inflammation associated with dyslipidemia and anti-oxidation in RAW264.7 and HUVEC cells. Methods: The total polyphenols, total flavonoids, DPPH radical scavenging activity, ABTS radical scavenging activity, and cytotoxicity of FMH were measured. RAW264.7 cells treated with FMH were tested for production of NO, and for cytokine and LTB4 levels and HUVEC cells treated with FMH were examined for production of cDNA of genes related to inflammation. Results: 1. FMH contained polyphenols and flavonoids. The DPPH and ABTS radical scavenging activity of FMH increased in a concentration-dependent manner. 2. FMH treatment inhibited the production of nitric oxide (NO), cytokines, and LTB4 in RAW264.7 cell when compared to the untreated control group. 3. FMH decreased the transcription of pro-inflammatory genes, whereas it increased transcription of anti-inflammatory genes, in HUVEC cells. Conclusion: FMH is effective as an antioxidant and for treatment and prevention of dyslipidemia, atherosclerosis, ischemic heart disease, stroke, and other cardiocerebrovascular diseases.

Abstract

Objectives: To investigate the effect of fermented extract of Artemisiae Iwayomogii Herba, Curcumae Longae, Crataegi Fructus, and Salviae Miltiorrhizae Radix (FMH) on anti-inflammation associated with dyslipidemia and anti-oxidation in RAW264.7 and HUVEC cells. Methods: The total polyphenols, total flavonoids, DPPH radical scavenging activity, ABTS radical scavenging activity, and cytotoxicity of FMH were measured. RAW264.7 cells treated with FMH were tested for production of NO, and for cytokine and LTB4 levels and HUVEC cells treated with FMH were examined for production of cDNA of genes related to inflammation. Results: 1. FMH contained polyphenols and flavonoids. The DPPH and ABTS radical scavenging activity of FMH increased in a concentration-dependent manner. 2. FMH treatment inhibited the production of nitric oxide (NO), cytokines, and LTB4 in RAW264.7 cell when compared to the untreated control group. 3. FMH decreased the transcription of pro-inflammatory genes, whereas it increased transcription of anti-inflammatory genes, in HUVEC cells. Conclusion: FMH is effective as an antioxidant and for treatment and prevention of dyslipidemia, atherosclerosis, ischemic heart disease, stroke, and other cardiocerebrovascular diseases.

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관련 논문AI추천

해백청혈플러스(AMCP)의 항산화 및 항염증 작용을 통한 죽상동맥경화 억제 효과김윤식, 설인찬, 유주영, 유호룡, 채인철한의병리학회 · 동의생리병리학회지2020 · 제34권 제3호茵蔯, 鬱金, 枳實 추출물(ACA)이 HepG2 세포에서 나타나는 이상지질혈증 관련 인자 발현 및 항산화에 미치는 영향김윤식, 설인찬, 유주영, 유호룡, 조현경대한한방내과학회 · 대한한방내과학회지2017 · 제38권 제3호HepG2 cell에서 한인진, 울금, 차전자 추출물(ACP)의 항산화 및 항이상지질혈증 효과김윤식, 설인찬, 유호룡, 정은선, 조현경한의병리학회 · 동의생리병리학회지2018 · 제32권 제1호db/db mouse에서 茵蔯, 鬱金, 山査 복합추출물의 항고지혈 및 항산화 효과김윤식, 김현태, 설인찬, 유호룡대한한방내과학회 · 대한한방내과학회지2016 · 제37권 제3호蘇木이 산화적 손상 및 Raw 264.7 cell과 HUVEC에서의 염증 유관 인자에 미치는 영향홍석, 강성순, 김명신, 양의호, 전상윤, 조재준, 최성안, 최창원대한한방내과학회 · 대한한방내과학회지2013 · 제34권 제1호三仁湯의 항산화 및Lipopolysaccharide로 유도된 RAW 264.7 대식세포에서의 항염증 효과고주휘, 김은서, 송시영, 이영수, 조수민대한한방내과학회 · 대한한방내과학회지2025 · 제46권 제4호

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