혈관내피세포에서 牛蒡子 에탄올 추출물의 항염증 효과
Anti-Inflammatory Effect of Ethanol Extract from the seeds of Arctium Lappa L. in Vascular Endothelial Cells
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Objectives: The seeds from Arctium lappa have been considered for its various pharmacological properties, which include anti-carcinogenic, anti-inflammatory, anti-diabetic, and anti-viral activities. Methods: In the present study, we investigated the anti-inflammatory effect of the ethanol extract from the seeds of Arctium lappa L (EAL) on cytokine-induced vascular inflammation in human umbilical vein endothelial cells (HUVEC). Results: Pretreatment with EAL significantly decreased tumor necrosis factor alpha (TNF-α)-induced cell adhesion molecules expression such as intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and endothelial-selectin (E-selectin) in a dose-dependent manner. Cell adhesion assay showed that pretreatment with EAL suppressed HUVEC-monocyte adhesion by TNF-α over 1 μg/ml concentration. We investigated the involvement of nuclear transcription factor kappa-B (NF-κB) in TNF-α-induced vascular inflammation. NF-κB p65 nuclear expression was induced by TNF-α, however, pretreatment with EAL was attenuated that nuclear translocation. In cytoplasm, EAL was also attenuated TNF-α-induced decrease of inhibitor of κB-α (IκB-α) expression. Moreover, EAL significantly decreased TNF-α-induced production of intracellular reactive oxygen species (ROS). Conclusions: Taken together, our findings suggest that seeds of Arctium lappa L could be a therapeutic herb for prevention of cardiovascular diseases throughout the inhibition of vascular endothelial inflammation.
Abstract
Objectives: The seeds from Arctium lappa have been considered for its various pharmacological properties, which include anti-carcinogenic, anti-inflammatory, anti-diabetic, and anti-viral activities. Methods: In the present study, we investigated the anti-inflammatory effect of the ethanol extract from the seeds of Arctium lappa L (EAL) on cytokine-induced vascular inflammation in human umbilical vein endothelial cells (HUVEC). Results: Pretreatment with EAL significantly decreased tumor necrosis factor alpha (TNF-α)-induced cell adhesion molecules expression such as intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and endothelial-selectin (E-selectin) in a dose-dependent manner. Cell adhesion assay showed that pretreatment with EAL suppressed HUVEC-monocyte adhesion by TNF-α over 1 μg/ml concentration. We investigated the involvement of nuclear transcription factor kappa-B (NF-κB) in TNF-α-induced vascular inflammation. NF-κB p65 nuclear expression was induced by TNF-α, however, pretreatment with EAL was attenuated that nuclear translocation. In cytoplasm, EAL was also attenuated TNF-α-induced decrease of inhibitor of κB-α (IκB-α) expression. Moreover, EAL significantly decreased TNF-α-induced production of intracellular reactive oxygen species (ROS). Conclusions: Taken together, our findings suggest that seeds of Arctium lappa L could be a therapeutic herb for prevention of cardiovascular diseases throughout the inhibition of vascular endothelial inflammation.
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