백부자 에탄올 추출물의 Streptococcus mutans에 대한 성장, 산생성, 부착 및 비수용성 글루칸 합성억제에 미치는 영향
Effects of Aconitum Koreanum Extract on the Growth, Acid Production, Adhesion and Insoluble Glucan Synthesis of Streptococcus Mutans
2
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
Streptococcus mutans triggers dental caries establishment by two major factors. One is synthesis of organic acids which demineralize dental enamel and the other is synthesis of glucans which mediate the attachment of bacteria to the tooth surface. In the present study, we evaluated the effect of the ethanol extracts of Aconitum koreanum (A. koreanum) on the growth and acid production of S. mutans. Ethanol extracts of the A. koreanum showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.016 mg/ml compared to the control groups (p<0.05). The extracts inhibited S. mutans adherence to hydroxyapatite treated with saliva, and cell adherence was repressed by 50%, 54% at the concentration of 0.063, 0.125 mg/ml. On the study of activation of glucosyltransferase which synthesizes water insoluble glucan form sucrose, the ethanol extract of A. koreanum showed remarkable inhibition over the concentration of 0.016, 0.031, 0.063 and 0.125 mg/ml (p<0.05). Especially on the concentration of 0.063, 0.125 mg/ml, the extracts suppressed the glucan synthesis by 100%. We analyzed the component of the extracts of A. koreanum. The results showed that the extract of A. koreanum had strong phenolic compound, glycosides and organic acids. These results suggest that A. koreanum may inhibit the caries-inducing properties of S. mutans, and which may be related with strong phenolic compound, glycosides and organic acids.
Abstract
Streptococcus mutans triggers dental caries establishment by two major factors. One is synthesis of organic acids which demineralize dental enamel and the other is synthesis of glucans which mediate the attachment of bacteria to the tooth surface. In the present study, we evaluated the effect of the ethanol extracts of Aconitum koreanum (A. koreanum) on the growth and acid production of S. mutans. Ethanol extracts of the A. koreanum showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.016 mg/ml compared to the control groups (p<0.05). The extracts inhibited S. mutans adherence to hydroxyapatite treated with saliva, and cell adherence was repressed by 50%, 54% at the concentration of 0.063, 0.125 mg/ml. On the study of activation of glucosyltransferase which synthesizes water insoluble glucan form sucrose, the ethanol extract of A. koreanum showed remarkable inhibition over the concentration of 0.016, 0.031, 0.063 and 0.125 mg/ml (p<0.05). Especially on the concentration of 0.063, 0.125 mg/ml, the extracts suppressed the glucan synthesis by 100%. We analyzed the component of the extracts of A. koreanum. The results showed that the extract of A. koreanum had strong phenolic compound, glycosides and organic acids. These results suggest that A. koreanum may inhibit the caries-inducing properties of S. mutans, and which may be related with strong phenolic compound, glycosides and organic acids.
참고문헌 (28)
- Oral health in America : a report of the surgeon general. Rockville, "U.S. Department of Health and Human Services(DHHS)", U.S. Department of Health and Human Service, ~ (2002)
- Crall, J.J, "Rethinking prevention", Pediatr Dent, 28, pp.96~101 (2006)
- Lee, S. K., "Reasons for extracted permanent teeth in Korean population", J. Korean Acad Dent Health, 25, pp.139~163 (2001)
- Chronic Disease, "2001 National Health and Nutritional Survey", Ministry of Health & Welfare, ~ (2002)
- 최인욱, "항충치효과를 가진 식물 소재 탐색", 한국식품과학회지, 35(6), pp.1221~1225 (2003), 한국식품과학회
- 이현옥, "고삼으로부터 항우식활성 물질의 분리", 한국미생물·생명공학회지, 30(4), pp.420~424 (2002), 한국미생물·생명공학회
- 김윤근, "호일카리성 Bacillus sp. 4830이 생산하는 Streptococcus mutans 세포벽 분해효소의 분리와 특성", 한국식품과학회지, 35(6), pp.1143~1149 (2003), 한국식품과학회
- Tsuneno, N., "Dental caries prevention by traditional chinese medicines.", Planta medica, 44, pp.100~106 (1982)
- 도동선, "충치원생세균 Streptococcus mutans OMZ 176에 대한 약용식물 추출물의 항균활성", 생약학회지, 33(4), pp.319~323 (2002), 한국생약학회
- Sohn, W. S., "The effect of NaCl and BAMBOO SALT on the growth of various oral bacteria", Journal of Academy of Oral Health, 15(2), pp.255~ (1991)
- Sakanaka, S., "Antibacterial substances in Japanese green tea extract against Streptococcus mutans, a cariogenic bacterium", Agric Biol Chem, 53, pp.2307~2311 (1989)
- Steinberg, D., "Antibacterial effect of propolis and honey on oral bacteria", Am J Dent, 9, pp.236~239 (1996)
- Chang, K. W., "Effect of dietary erythritol supplemented with chitosan, extracts of Akebia and extracts of Ishige on the growth of mutans streptococci", Journal of Academy of Oral Health, 21(3), pp.545~552 (1997)
- Chang, K. W., "Antibacterial effect and adsorption inhibition of oral streptococci to saliva - coated hydroxyapatite beads with Akebia quinata extract", Journal of Academy of Oral Health, 21(4), pp.675~684 (1997)
- Chang, K. W., "Antibacterial effects of Sargassum horneri extract to the Streptococcus mutans and Streptococcus", Journal of Academy of Oral Health, 21(2), pp.379~388 (1997)
- Yang, J. Y., "Antimicrobial Effect of Mustard, Cinnamon, Japanese Pepper and Horseradish", Journal of Food Hygiene and Safety, 16(1), pp.37~41 (2001)
- Im, R.J., "In Flora medica coreana: Part traditional medicine", pp.171~172 (1998), Pyongyang
- Ann, D.G, "In Illustrated book of korean medicinal herbs", pp.598~599 (1998), Kyohak Publishing Co., LTD.
- Jang, I.S., "East pharmacist reference set", pp.320~321 (1993), YeoGang Publisher
- Moon, G.S., "Comprehensive Dictionary of Scientific Encyclopedia Publishing collection. Components and use of herbs", pp.223~225 (1991), ilwolbooks
- Cha, J.H., "omprehensive Dictionary of Scientific Encyclopedia Publishing collection. (Practical use)Consent pharmacy", pp.459~460 (1990), ilwolbooks
- Yang, C.Y, "In Poisonous drug medical herb", pp.191~197 (1995), chinese public opinion crane publishing company
- Park, S. Y., "Studies on the preparation of processde Aconiti tubers", Kor. J. Pharmacogn, 20, pp.25~31 (1989)
- 윤소정, "추출용매 비에 따른 백부자(Aconiti koreani Rhizoma) 추출물의항균효과 및 항산화효과", Applied Biological Chemistry, 48(3), pp.258~262 (2005), 한국응용생명화학회
- Jones, C. L., "The effect of longterm use of a dentifrice containing zinc citrate and a non-ionic agent on the oral flora", J Dent, 67, pp.46~50 (1988)
- Min, T. J., "The Structure of Phenolic Compounds and Their Antibiotic Activities in Umbilicaria vellea", Journal of the Korean Chemical Society, 40(9), pp.623~630 (1996)
- Chuyen, N. V., "Antimicrobial activity of kumazasa (Susa albomarinata)", Agric biology chemistry, 46, pp.971~978 (1982)
- Lee, D. K., "The Stability and Mutagenecity of β-Sitosterol Glycoside, Antimicrobial Compound from Schima wallichii sp. liukiuensis", The Journal of Applied Phamacology, 7(3), pp.250~209 (1999)
