본문 바로가기
KERD
논문KCI 등재

백부자 에탄올 추출물의 Streptococcus mutans에 대한 성장, 산생성, 부착 및 비수용성 글루칸 합성억제에 미치는 영향

Effects of Aconitum Koreanum Extract on the Growth, Acid Production, Adhesion and Insoluble Glucan Synthesis of Streptococcus Mutans

2

조회

0

다운로드

6

인용

0

참고문헌

키워드

주제분류한의학

초록

Streptococcus mutans triggers dental caries establishment by two major factors. One is synthesis of organic acids which demineralize dental enamel and the other is synthesis of glucans which mediate the attachment of bacteria to the tooth surface. In the present study, we evaluated the effect of the ethanol extracts of Aconitum koreanum (A. koreanum) on the growth and acid production of S. mutans. Ethanol extracts of the A. koreanum showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.016 mg/ml compared to the control groups (p<0.05). The extracts inhibited S. mutans adherence to hydroxyapatite treated with saliva, and cell adherence was repressed by 50%, 54% at the concentration of 0.063, 0.125 mg/ml. On the study of activation of glucosyltransferase which synthesizes water insoluble glucan form sucrose, the ethanol extract of A. koreanum showed remarkable inhibition over the concentration of 0.016, 0.031, 0.063 and 0.125 mg/ml (p<0.05). Especially on the concentration of 0.063, 0.125 mg/ml, the extracts suppressed the glucan synthesis by 100%. We analyzed the component of the extracts of A. koreanum. The results showed that the extract of A. koreanum had strong phenolic compound, glycosides and organic acids. These results suggest that A. koreanum may inhibit the caries-inducing properties of S. mutans, and which may be related with strong phenolic compound, glycosides and organic acids.

Abstract

Streptococcus mutans triggers dental caries establishment by two major factors. One is synthesis of organic acids which demineralize dental enamel and the other is synthesis of glucans which mediate the attachment of bacteria to the tooth surface. In the present study, we evaluated the effect of the ethanol extracts of Aconitum koreanum (A. koreanum) on the growth and acid production of S. mutans. Ethanol extracts of the A. koreanum showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.016 mg/ml compared to the control groups (p<0.05). The extracts inhibited S. mutans adherence to hydroxyapatite treated with saliva, and cell adherence was repressed by 50%, 54% at the concentration of 0.063, 0.125 mg/ml. On the study of activation of glucosyltransferase which synthesizes water insoluble glucan form sucrose, the ethanol extract of A. koreanum showed remarkable inhibition over the concentration of 0.016, 0.031, 0.063 and 0.125 mg/ml (p<0.05). Especially on the concentration of 0.063, 0.125 mg/ml, the extracts suppressed the glucan synthesis by 100%. We analyzed the component of the extracts of A. koreanum. The results showed that the extract of A. koreanum had strong phenolic compound, glycosides and organic acids. These results suggest that A. koreanum may inhibit the caries-inducing properties of S. mutans, and which may be related with strong phenolic compound, glycosides and organic acids.

참고문헌 (28)

  1. Oral health in America : a report of the surgeon general. Rockville, "U.S. Department of Health and Human Services(DHHS)", U.S. Department of Health and Human Service, ~ (2002)
  2. Crall, J.J, "Rethinking prevention", Pediatr Dent, 28, pp.96~101 (2006)
  3. Lee, S. K., "Reasons for extracted permanent teeth in Korean population", J. Korean Acad Dent Health, 25, pp.139~163 (2001)
  4. Chronic Disease, "2001 National Health and Nutritional Survey", Ministry of Health & Welfare, ~ (2002)
  5. 최인욱, "항충치효과를 가진 식물 소재 탐색", 한국식품과학회지, 35(6), pp.1221~1225 (2003), 한국식품과학회
  6. 이현옥, "고삼으로부터 항우식활성 물질의 분리", 한국미생물·생명공학회지, 30(4), pp.420~424 (2002), 한국미생물·생명공학회
  7. 김윤근, "호일카리성 Bacillus sp. 4830이 생산하는 Streptococcus mutans 세포벽 분해효소의 분리와 특성", 한국식품과학회지, 35(6), pp.1143~1149 (2003), 한국식품과학회
  8. Tsuneno, N., "Dental caries prevention by traditional chinese medicines.", Planta medica, 44, pp.100~106 (1982)
  9. 도동선, "충치원생세균 Streptococcus mutans OMZ 176에 대한 약용식물 추출물의 항균활성", 생약학회지, 33(4), pp.319~323 (2002), 한국생약학회
  10. Sohn, W. S., "The effect of NaCl and BAMBOO SALT on the growth of various oral bacteria", Journal of Academy of Oral Health, 15(2), pp.255~ (1991)
  11. Sakanaka, S., "Antibacterial substances in Japanese green tea extract against Streptococcus mutans, a cariogenic bacterium", Agric Biol Chem, 53, pp.2307~2311 (1989)
  12. Steinberg, D., "Antibacterial effect of propolis and honey on oral bacteria", Am J Dent, 9, pp.236~239 (1996)
  13. Chang, K. W., "Effect of dietary erythritol supplemented with chitosan, extracts of Akebia and extracts of Ishige on the growth of mutans streptococci", Journal of Academy of Oral Health, 21(3), pp.545~552 (1997)
  14. Chang, K. W., "Antibacterial effect and adsorption inhibition of oral streptococci to saliva - coated hydroxyapatite beads with Akebia quinata extract", Journal of Academy of Oral Health, 21(4), pp.675~684 (1997)
  15. Chang, K. W., "Antibacterial effects of Sargassum horneri extract to the Streptococcus mutans and Streptococcus", Journal of Academy of Oral Health, 21(2), pp.379~388 (1997)
  16. Yang, J. Y., "Antimicrobial Effect of Mustard, Cinnamon, Japanese Pepper and Horseradish", Journal of Food Hygiene and Safety, 16(1), pp.37~41 (2001)
  17. Im, R.J., "In Flora medica coreana: Part traditional medicine", pp.171~172 (1998), Pyongyang
  18. Ann, D.G, "In Illustrated book of korean medicinal herbs", pp.598~599 (1998), Kyohak Publishing Co., LTD.
  19. Jang, I.S., "East pharmacist reference set", pp.320~321 (1993), YeoGang Publisher
  20. Moon, G.S., "Comprehensive Dictionary of Scientific Encyclopedia Publishing collection. Components and use of herbs", pp.223~225 (1991), ilwolbooks
  21. Cha, J.H., "omprehensive Dictionary of Scientific Encyclopedia Publishing collection. (Practical use)Consent pharmacy", pp.459~460 (1990), ilwolbooks
  22. Yang, C.Y, "In Poisonous drug medical herb", pp.191~197 (1995), chinese public opinion crane publishing company
  23. Park, S. Y., "Studies on the preparation of processde Aconiti tubers", Kor. J. Pharmacogn, 20, pp.25~31 (1989)
  24. 윤소정, "추출용매 비에 따른 백부자(Aconiti koreani Rhizoma) 추출물의항균효과 및 항산화효과", Applied Biological Chemistry, 48(3), pp.258~262 (2005), 한국응용생명화학회
  25. Jones, C. L., "The effect of longterm use of a dentifrice containing zinc citrate and a non-ionic agent on the oral flora", J Dent, 67, pp.46~50 (1988)
  26. Min, T. J., "The Structure of Phenolic Compounds and Their Antibiotic Activities in Umbilicaria vellea", Journal of the Korean Chemical Society, 40(9), pp.623~630 (1996)
  27. Chuyen, N. V., "Antimicrobial activity of kumazasa (Susa albomarinata)", Agric biology chemistry, 46, pp.971~978 (1982)
  28. Lee, D. K., "The Stability and Mutagenecity of β-Sitosterol Glycoside, Antimicrobial Compound from Schima wallichii sp. liukiuensis", The Journal of Applied Phamacology, 7(3), pp.250~209 (1999)

관련 논문AI추천

백두옹 추출물이 Streptococcus mutans의 비수용성 글루칸형성 및 부착억제에 미치는 영향김강주, 민재홍, 박복임, 손혁진, 안소연, 유용욱, 이기훈, 임재유, 전병훈, 채민석, 최나영한의병리학회 · 동의생리병리학회지2016 · 제30권 제1호구맥 추출물의 Streptococcus mutans 활성 억제 효능김동규, 김진국, 유용욱, 유현희한의병리학회 · 동의생리병리학회지2010 · 제24권 제5호목향 에탄올 추출물의 Streptococcus mutans에 대한 성장, 산생성, 부착 및 비수용성 글루칸 합성 억제에 미치는 영향김연화, 소홍섭, 유용욱, 유현희, 이기현, 이준섭, 전병훈한의병리학회 · 동의생리병리학회지2005 · 제19권 제5호오배자 Galla Chinensis 추출물이 Streptococcus mutans의 우식활성 억제에 미치는 영향김강주, 김정선, 박복임, 안소연, 유성진, 유용욱, 이찬우, 전병훈, 정원창한의병리학회 · 동의생리병리학회지2015 · 제29권 제2호황금(Scutellaria baicalensis) 추출물에 의한 Streptococcus mutans의 항균 및 부착억제 효과권현정, 김완종, 김용현, 김은님, 백종윤, 한만덕한국치위생과학회 · 치위생과학회지2008 · 제8권 제4호Inhibitory Effect of Ethanol Extract of Angelica gigas Nakai on Biofilm Formation and Metabolic Activity of Streptococcus mutans according to Xylitol and SucroseDo-Seon Lim, Kyungwon Heo, Moon-Jin Jeong, Soon-Jeong Jeong, Sung Ok Kim한국치위생과학회 · 치위생과학회지2024 · 제24권 제4호

이 간행물의 다른 논문 (14건)