비소화합물에 의한 A549 폐암세포의 증식억제에 관한 연구
Effects of Arsenic Compounds (As2O3 and As4O6) on the Induction of Apoptotic Cell Death in A549 Human Non-small Cell Lung Cancer Cells
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Abstract
Recently, arsenic compounds were considered as novel agents for treatment of acute promyelocytic leukemia and malignant tumors. However, it showed severe toxicity effect on normal tissue at the same time. In this study, to investigate the possible molecular mechanism (s) of arsenic compounds as candidate of anti-cancer drugs, we compared the abilities of two arsenic compounds, tetraarsenic oxide (As4O6) and arsenic trioxide (diarsenic oxide, As2O3), to induce cell growth inhibition as well as apoptosis induction in A549 human non-small cell lung cancer cells. Both As4O6 and As2O3 treatment declined the cell growth and viability of A549 cells in a concentration-dependent manner, which was associated with induction of G1 arrest of the cell cycle and apoptotic cell death. However, As4O6 induced growth inhibition and apoptosis in A549 cells at much lower concentrations than As2O3. As4O6 down-regulated the levels of anti-apoptotic Bcl-2 protein, however, the levels of Bax, a pro-apoptotic protein, were up-regulated in a dose-dependent manner. In conclusion, As4O6 might be a new arsenic compound which may induce apoptosis in A549 cells by modulation the Bcl-2 family and deserves further evaluation.
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