Surface expression of TTYH2 is attenuated by direct interaction with β-COP
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TTYH2 is a calcium-activated, inwardly rectifying anion channel that has been shown to be related to renal cancer and colon cancer. Based on the topological prediction, TTYH2 protein has five transmembrane domains with the extracellular N-terminus and the cytoplasmic C-terminus. In the present study, we identified a vesicle transport protein, β-COP, as a novel specific binding partner of TTYH2 by yeast two-hybrid screening using a human brain cDNA library with the C-terminal region of TTYH2 (TTYH2-C) as a bait. Using in vitro and in vivo binding assays, we confirmed the protein-protein interactions between TTYH2 and β-COP. We also found that the surface expression and activity of TTYH2 were decreased by co-expression with β-COP in the heterologous expression system. In addition, β-COP associated with TTYH2 in a native condition at a human colon cancer cell line, LoVo cells. The over-expression of β-COP in the LoVo cells led to a dramatic decrease in the surface expression and activity of endogenous TTYH2. Collectively, these data suggested that β-COP plays a critical role in the trafficking of the TTYH2 channel to the plasma membrane.
Abstract
TTYH2 is a calcium-activated, inwardly rectifying anion channel that has been shown to be related to renal cancer and colon cancer. Based on the topological prediction, TTYH2 protein has five transmembrane domains with the extracellular N-terminus and the cytoplasmic C-terminus. In the present study, we identified a vesicle transport protein, β-COP, as a novel specific binding partner of TTYH2 by yeast two-hybrid screening using a human brain cDNA library with the C-terminal region of TTYH2 (TTYH2-C) as a bait. Using in vitro and in vivo binding assays, we confirmed the protein-protein interactions between TTYH2 and β-COP. We also found that the surface expression and activity of TTYH2 were decreased by co-expression with β-COP in the heterologous expression system. In addition, β-COP associated with TTYH2 in a native condition at a human colon cancer cell line, LoVo cells. The over-expression of β-COP in the LoVo cells led to a dramatic decrease in the surface expression and activity of endogenous TTYH2. Collectively, these data suggested that β-COP plays a critical role in the trafficking of the TTYH2 channel to the plasma membrane.
참고문헌 (27)
- Campbell HD, "The Drosophila melanogaster flightless-I gene involved in gastrulation and muscle degeneration encodes gelsolin-like and leucine-rich repeat domains and is conserved in Caenorhabditis elegans and humans", Proc Natl Acad Sci U S A, 90, pp.11386~11390 (1993)
- Suzuki M, "A novel human Cl(-)channel family related to Drosophila flightless locus", J Biol Chem, 279, pp.22461~22468 (2004)
- Rae FK, "TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is located on 17q24 and upregulated in renal cell carcinoma", Genomics, 77, pp.200~207 (2001)
- Toiyama Y, "TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation", World J Gastroenterol, 13, pp.2717~2721 (2007)
- Dohna M, "Adrenocortical carcinoma is characterized by a high frequency of chromosomal gains and high-level amplifications", Genes Chromosomes Cancer, 28, pp.145~152 (2000)
- 조장훈, "Physiological functions of the TRPM4 channels via protein interactions", BMB Reports, 48(1), pp.1~5 (2015), 생화학분자생물학회
- Bethune J, "COPI-mediated transport", J Membr Biol, 211, pp.65~79 (2006)
- Hu CD, "Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation", Mol Cell, 9, pp.789~798 (2002)
- 최종현, "Functional roles of glutamic acid E143 and E705 residues in the N-terminus and transmembrane domain 7 of Anoctamin 1 in calcium and noxious heat sensing", BMB Reports, 51(5), pp.236~241 (2018), 생화학분자생물학회
- Kim E, "Enhancement of TREK1 channel surface expression by protein-protein interaction with beta-COP", Biochem Biophys Res Commun, 395, pp.244~250 (2010)
- Lee YS, "Surface expression of the Anoctamin-1(ANO1)channel is suppressed by protein-protein interactions with beta-COP", Biochem Biophys Res Commun, 475, pp.216~222 (2016)
- O'Kelly I, "Forward transport. 14-3-3 binding overcomes retention in endoplasmic reticulum by dibasic signals", Cell, 111, pp.577~588 (2002)
- Zuzarte M, "Intracellular traffic of the K+ channels TASK-1 and TASK-3 : role of N-and C-terminal sorting signals and interaction with 14-3-3 proteins", J Physiol, 587, pp.929~952 (2009)
- Rennolds J, "Cystic fibrosis transmembrane conductance regulator trafficking is mediated by the COPI coat in epithelial cells", J Biol Chem, 283, pp.833~839 (2008)
- Aoe T, "Modulation of intracellular transport by transported proteins : insight from regulation of COPI-mediated transport", Proc Natl Acad Sci U S A, 95, pp.1624~1629 (1998)
- Ma W, "Rules for the recognition of dilysine retrieval motifs by coatomer", EMBO J, 32, pp.926~937 (2013)
- Letourneur F, "Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum", Cell, 79, pp.1199~1207 (1994)
- Khalil H, "A three-aminoacid-long HLA-DRbeta cytoplasmic tail is sufficient to overcome ER retention of invariant-chain p35", J Cell Sci, 118, pp.4679~4687 (2005)
- Peretti M, "Chloride channels in cancer : Focus on chloride intracellular channel 1 and 4(CLIC1 AND CLIC4)proteins in tumor development and as novel therapeutic targets", Biochim Biophys Acta, 1848, pp.2523~2531 (2015)
- Mao JW, "Volume-activated Cl-current in migrated nasopharyngeal carcinoma cells", Sheng Li Xue Bao, 56, pp.525~530 (2004)
- Xu B, "ClC-3 chloride channels are essential for cell proliferation and cell cycle progression in nasopharyngeal carcinoma cells", Acta Biochim Biophys Sin (Shanghai), 42, pp.370~380 (2010)
- Steele DF, "Mechanisms of cardiac potassium channel trafficking", J Physiol, 582, pp.17~26 (2007)
- Lee YS, "Suppression of 14-3-3γ-mediated surface expression of ANO1 inhibits cancer progression of glioblastoma cells", Sci Rep, 6, pp.26413~ (2016)
- Wang X, "Hsp90cochaperone Aha1 downregulation rescues misfolding of CFTR in cystic fibrosis", Cell, 127, pp.803~815 (2006)
- Perez-Cornejo P, "Anoctamin 1(Tmem16A)Ca2+-activated chloride channel stoichiometrically interacts with an ezrin-radixin-moesin network", Proc Natl Acad Sci U S A, 109, pp.10376~10381 (2012)
- Chatr-Aryamontri A, "The BioGRID interaction databse : 2017 update", Nucleic Acids Res, 45, pp.D369~D379 (2017)[DOI]
- Kim J, "Ttyh1 regulates embryonic neural stem cell properties by enhancing the notch signaling pathway", EMBO Rep, 19, pp.e45472~ (2018)