Ileal Endogenous Amino Acid Flow Response to Nitrogen-free Diets with Differing Ratios of Corn Starch to Dextrose in Pigs
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The objective of this study was to determine the responses in the digestibility of dry matter (DM) and amino acid (AA) composition of ileal endogenous flow (IEF) of pigs (initial body weight, 69.1±6.46 kg) fed N-free diets (NFD) formulated with different ratios of corn starch to dextrose. Fifteen pigs fitted with a T-cannula at the distal ileum were fed 5 diets according to a triplicated 5×2 incomplete Latin-square design. Each period consisted of a 5-d adjustment period and 2 d of ileal digesta collection for 12 h on each of d 6 and 7 and between each period, there was a 5-d recovery period to avoid abnormal weight loss. The ratios of corn starch to dextrose investigated were 0:879, 293:586, 586:293, 779:100, and 879:0 for diet numbers 1, 2, 3, 4 and 5, respectively, and chromic oxide (5 g/kg) was used as an indigestible index. Ileal DM digestibility was greater in Diet 1 than that in Diet 4 (89.5% vs 87.3%, p<0.01) but they were not different from Diet 2, 3, or 5. The IEF for most of indispensable AA were not different among diets with the exception of Met, in which a lack of corn starch or dextrose gave lower (p = 0.028) IEF of Met than diets containing corn starch and dextrose. Likewise, the dispensable AA and total AA in the IEF did not differ among diets. The respective IEF of AA (mg/kg of dry matter intake) in pigs fed Diets 1, 2, 3, 4, or 5 were 301, 434, 377, 477,or 365 for Lys, 61, 89, 71, 87, or 61 for Met, and 477, 590, 472, 520, or 436 for Thr. Proline was the most abundant AA in the IEF followed by Gly, Glu, and Asp and together accounted for approximately 50% of the total ileal AA flows of pigs fed NFD. In conclusion, the variation in proportion of corn starch and dextrose in a NFD does not largely affect estimates of IEF of N and AA for growing-finishing pigs.
Abstract
The objective of this study was to determine the responses in the digestibility of dry matter (DM) and amino acid (AA) composition of ileal endogenous flow (IEF) of pigs (initial body weight, 69.1±6.46 kg) fed N-free diets (NFD) formulated with different ratios of corn starch to dextrose. Fifteen pigs fitted with a T-cannula at the distal ileum were fed 5 diets according to a triplicated 5×2 incomplete Latin-square design. Each period consisted of a 5-d adjustment period and 2 d of ileal digesta collection for 12 h on each of d 6 and 7 and between each period, there was a 5-d recovery period to avoid abnormal weight loss. The ratios of corn starch to dextrose investigated were 0:879, 293:586, 586:293, 779:100, and 879:0 for diet numbers 1, 2, 3, 4 and 5, respectively, and chromic oxide (5 g/kg) was used as an indigestible index. Ileal DM digestibility was greater in Diet 1 than that in Diet 4 (89.5% vs 87.3%, p<0.01) but they were not different from Diet 2, 3, or 5. The IEF for most of indispensable AA were not different among diets with the exception of Met, in which a lack of corn starch or dextrose gave lower (p = 0.028) IEF of Met than diets containing corn starch and dextrose. Likewise, the dispensable AA and total AA in the IEF did not differ among diets. The respective IEF of AA (mg/kg of dry matter intake) in pigs fed Diets 1, 2, 3, 4, or 5 were 301, 434, 377, 477,or 365 for Lys, 61, 89, 71, 87, or 61 for Met, and 477, 590, 472, 520, or 436 for Thr. Proline was the most abundant AA in the IEF followed by Gly, Glu, and Asp and together accounted for approximately 50% of the total ileal AA flows of pigs fed NFD. In conclusion, the variation in proportion of corn starch and dextrose in a NFD does not largely affect estimates of IEF of N and AA for growing-finishing pigs.
참고문헌 (25)
- Adedokun, S. A., "Factors affecting endogenous amino acid flow in chickens and the need for consistency in methodology", Poult. Sci, 90, pp.1737~1748 (2011)
- AOAC, "Official Methods of Analysis" (2006), Association Official Analytical Chemists
- Bennick, A., "Salivary proline-rich proteins", Mol. Cell. Biochem, 45, pp.83~99 (1982)
- Cozannet, P., "Ileal digestibility of amino acids in wheat distillers dried grains with solubles for pigs", Anim. Feed Sci. Technol, 158, pp.177~186 (2010)
- "Corn starch" (2013)[URL]
- Lange, C. F. M., "The effect of feeding different protein-free amino acid composition of endogenous protein collected from the distal ileum and feces in pigs", J. Anim. Sci, 67, pp.746~754 (1989)
- Dilger, R. N., "Digestibility of nitrogen and amino acids in soybean meal with added soyhulls", J. Anim. Sci, 82, pp.715~724 (2004)
- Fenton, T. W., "An improved procedure for the determination of chromic oxide in feed and feces", Can. J. Anim. Sci, 59, pp.631~634 (1979)
- Hofmann, A. F., "Bile salts of vertebrates: structural variation and possible evolutionary significance", J. Lipid Res, 51, pp.226~246 (2010)
- Hughes, R. J., "Relationship between digesta transit time and apparent metabolisable energy value of wheat in chickens", Br. Poult. Sci, 49, pp.716~720 (2008)
- Jansman, A. J. M., "Evaluation through literature data of the amount and amino acid composition of basal endogenous crude protein at the terminal ileum of pigs", Anim. Feed Sci. Technol, 98, pp.49~60 (2002)
- Kim, B. G., "The correlation between passage rate of digesta and dry matter digestibility in various stages of swine", Livest. Sci, 109, pp.81~84 (2007)
- Kong, C., "Ileal endogenous amino acid flow response to nitrogen-free diets with differing ratios of corn starch to dextrose in broiler chickens", Poult. Sci, 92, pp.1276~1282 (2013)
- Li, S. F., "Energy, amino acid, and phosphorus digestibility of phytase transgenic corn for growing pigs", J. Anim. Sci, 91, pp.298~308 (2013)
- Lien, K. A., "Mucin output in ileal digesta of pigs fed a protein-free diet", Z. Ernährungswiss, 36, pp.182~190 (1997)
- Moughan, P. J., "Gut luminal endogenous protein: Implications for the determination of ileal amino acid digestibility in humans", Br. J. Nutr, 108, pp.S258~S263 (2012)
- NRC, "Nutrient Requirements of Swine" (1998), National Academy Press
- Nyachoti, C. M., "Significance of endogenous gut nitrogen losses in the nutrition of growing pigs: A review", Can. J. Anim. Sci, 77, pp.149~163 (1997)
- Park, C. S, "Prediction of basal endogenous losses of amino acids based on body weight and feed intake in pigs fed nitrogen-free diets", Rev. Colomb. Cienc. Pecu, 26, pp.186~192 (2013)
- Rothman, S., "Conservation of digestive enzymes", Physiol. Rev, 82, pp.1~18 (2002)
- Stein, H. H., "Additivity of values for apparent and standardized ileal digestibility of amino acids in mixed diets fed to growing pigs", J. Anim. Sci, 83, pp.2387~2395 (2005)
- Stein, H. H., "Invited review: Amino acid bioavailability and digestibility in pig feed ingredients: Terminology and application", J. Anim. Sci, 85, pp.172~180 (2007)
- Wilfart, A., "Digesta transit in different segments of the gastrointestinal tract of pigs as affected by insoluble fibre supplied by wheat bran", Br. J. Nutr, 98, pp.54~62 (2007)
- Zhai, H., "Apparent and standardized ileal digestibilities of amino acids for pigs fed corn- and soybean meal-based diets at varying crude protein levels", J. Anim. Sci, 89, pp.3626~3633 (2011)
- Yongcheng Zhang, "Effects of Casein and Protein-free Diets on Endogenous Amino Acid Losses in Pigs", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 15(11), pp.1634~1638 (2002), 아세아·태평양축산학회