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Differential Impacts on Bacterial Composition and Abundance in Rhizosphere Compartments between Al-Tolerant and Al-Sensitive Soybean Genotypes in Acidic Soil

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주제분류생물학

초록

In this study, two soybean genotypes, i.e., aluminum-tolerant Baxi 10 (BX10) and aluminumsensitive Bendi 2 (BD2), were used as plant materials and acidic red soil was used as growth medium. The soil layers from the inside to the outside of the root are: rhizospheric soil after washing (WRH), rhizospheric soil after brushing (BRH) and rhizospheric soil at two sides (SRH), respectively. The rhizosphere bacterial communities were analyzed by high-throughput sequencing of V4 hypervariable regions of 16S rRNA gene amplicons via Illumina MiSeq. The results of alpha diversity analysis showed that the BRH and SRH of BX10 were significantly lower in community richness than that of BD2, while the WRH exhibited no significant difference between BX10 and BD2. Among the three sampling compartments of the same soybean genotype, WRH had the lowest community richness and diversity while showing the highest coverage. Beta diversity analysis results displayed no significant difference for any compartment between the two genotypes, or among the three different sampling compartments for any same soybean genotype. However, the relative abundance of major bacterial taxa, specifically nitrogen-fixing and/or aluminum-tolerant bacteria, was significantly different in the compartments of the BRH and/or SRH at phylum and genus levels, indicating genotype-dependent variations in rhizosphere bacterial communities. Strikingly, as compared with BRH and SRH, the WRH within the same genotype (BX10 or BD2) always had an enrichment effect on rhizosphere bacteria associated with nitrogen fixation

Abstract

In this study, two soybean genotypes, i.e., aluminum-tolerant Baxi 10 (BX10) and aluminumsensitive Bendi 2 (BD2), were used as plant materials and acidic red soil was used as growth medium. The soil layers from the inside to the outside of the root are: rhizospheric soil after washing (WRH), rhizospheric soil after brushing (BRH) and rhizospheric soil at two sides (SRH), respectively. The rhizosphere bacterial communities were analyzed by high-throughput sequencing of V4 hypervariable regions of 16S rRNA gene amplicons via Illumina MiSeq. The results of alpha diversity analysis showed that the BRH and SRH of BX10 were significantly lower in community richness than that of BD2, while the WRH exhibited no significant difference between BX10 and BD2. Among the three sampling compartments of the same soybean genotype, WRH had the lowest community richness and diversity while showing the highest coverage. Beta diversity analysis results displayed no significant difference for any compartment between the two genotypes, or among the three different sampling compartments for any same soybean genotype. However, the relative abundance of major bacterial taxa, specifically nitrogen-fixing and/or aluminum-tolerant bacteria, was significantly different in the compartments of the BRH and/or SRH at phylum and genus levels, indicating genotype-dependent variations in rhizosphere bacterial communities. Strikingly, as compared with BRH and SRH, the WRH within the same genotype (BX10 or BD2) always had an enrichment effect on rhizosphere bacteria associated with nitrogen fixation

참고문헌 (55)

  1. Vonuexkull HR, "Global extent, development and economic-impact of acid soils", Plant Soil, 171, pp.1~15 (1995)
  2. Ryan PR, "Aluminum toxicity in roots-Correlation among ionic currents, ion fluxes, and root elongation in aluminum-sensitive and aluminum-tolerant wheat cultivars", Plant Physiol, 99, pp.1193~1200 (1992)
  3. Koyama H, "Effects of aluminum and Ph on root-growth and cell viability in Arabidopsis-Thaliana strain landsberg in hydroponic culture", Plant Cell Physiol, 36, pp.201~205 (1995)
  4. Kochian LV, "Cellular mechanisms of aluminum toxicity and resistance in plants", Annu. Rev. Plant Phys, 46, pp.237~260 (1995)
  5. Ryan PR, "Function and mechanism of organic anion exudation from plant roots", Annu. Rev. Plant Phys, 52, pp.527~560 (2001)
  6. Wei LL, "Citric acid enhances the mobilization of organic phosphorus in subtropical and tropical forest soils", Biol. Fertil. Soils, 46, pp.765~769 (2010)
  7. Ma JF, "Aluminium tolerance in plants and the complexing role of organic acids", Trends Plant Sci, 6, pp.273~278 (2001)
  8. Cheng FX, "Isolation and application of effective nitrogen fixation rhizobial strains on low-phosphorus acid soils in South China", Chinese Sci. Bull, 54, pp.412~420 (2009)
  9. Dong DF, "Organic acid exudation induced by phosphorus deficiency and", or aluminium toxicity in two contrasting soybean genotypes, 2004, pp.122, 190~199
  10. Yang TY, "Rhizosphere microbial communities and organic acids secreted by aluminum-tolerant and aluminum-sensitive soybean in acid soil", Biol. Fertil. Soils, 48, pp.97~108 (2012)
  11. Jaeger CH, "Mapping of sugar and amino acid availability in soil around roots with bacterial sensors of sucrose and Tryptophan", Appl. Environ. Microbiol, 65, pp.2685~2690 (1999)
  12. Berg G, "The rhizosphere as a reservoir for opportunistic human pathogenic bacteria", Environ. Microbiol, 7, pp.1673~1685 (2005)
  13. Bulgarelli D, "Structure and functions of the bacterial microbiota of plants", Annu. Rev. Plant Biol, 64, pp.807~838 (2013)
  14. Bais HP, "The role of root exudates in rhizosphere interations with plants and other organisms", Annu. Rev. Plant Biol, 57, pp.233~266 (2006)
  15. Paterson E, "Rhizodeposition shapes rhizosphere microbial community structure in organic soil", New Phytol, 173, pp.600~610 (2007)
  16. Aira M, "Plant genotype strongly modifies the structure and growth of maize rhizosphere microbial communities", Soil Biol. Biochem, 42, pp.2276~2281 (2010)
  17. Kondorosi E, "A Paradigm for endosymbiotic life : Cell differentiation of rhizobium bacteria provoked by host plant factors", Annu. Rev. Microbiol, 67, pp.611~628 (2013)
  18. Ofek M, "Host signature effect on plant root-associated microbiomes revealed through analyses of resident vs. active communities", Environ. Microbiol., 16, pp.2157~2167 (2014)
  19. Lugtenberg B, "Plant-growth-promoting rhizobacteria", Annu. Rev. Microbiol, 63, pp.541~556 (2009)
  20. Berendsen RL, "The rhizosphere microbiome and plant health", Trends Plant Sci, 17, pp.478~486 (2012)
  21. Berg G, "Unraveling the plant nnicrobiome : looking back and future perspectives", Front. Microbiol, 5, pp.148~ (2014)
  22. Li YC, "Differential responses of the diazotrophic community to aluminum-tolerant and aluminum-sensitive soybean genotypes in acidic soil", Eur. J. Soil Biol, 53, pp.76~85 (2012)
  23. Yang T, "Rhizosphere bacteria induced by aluminum-tolerant and aluminumsensitive soybeans in acid soil", Plant Soil Environ, 58, pp.262~267 (2012)
  24. Li YL, "The relationship between rhizosphere nitrification and nitrogen-use efficiency in rice plants", Plant Cell Environ, 31, pp.73~85 (2008)
  25. Inceoglu O, "Effects of plant genotype and growth stage on the betaproteobacterial communities associated with different potato cultivars in two fields", Appl. Environ. Microbiol, 76, pp.3675~3684 (2010)
  26. Lu GH, "Effects of an EPSPS-transgenic soybean line ZUTS31 on rootassociated bacterial communities during field growth", PLoS One, 13, pp.e0192008~ (2018)
  27. Wen ZL, "Enrichments", Derichments of root-associated bacteria related to plant growth and nutrition caused by the growth of an EPSPS-Transgenic maize line in the field, 2019, pp.10, 1335~
  28. Gui-Hua Lu, "Impact of a Glyphosate-Tolerant Soybean Line on the Rhizobacteria, Revealed by Illumina MiSeq", Journal of Microbiology and Biotechnology, 27(3), pp.561~572 (2017), 한국미생물·생명공학회[DOI]
  29. Kennedy K, "Evaluating bias of illumina-based bacterial 16S rRNA gene profiles", Appl. Environ. Microbiol, 80, pp.5717~5722 (2014)
  30. Kozich JJ, "Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform", Appl. Environ. Microbiol, 79, pp.5112~5120 (2013)
  31. Caporaso JG, "Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms", ISME J, 6, pp.1621~1624 (2012)
  32. Edgar RC, "UPARSE : highly accurate OTU sequences from microbial amplicon reads", Nat. Methods, 10, pp.996~998 (2013)
  33. Edgar RC, "UCHIME improves sensitivity and speed of chimera detection", Bioinformatics, 27, pp.2194~2200 (2011)
  34. Caporaso JG, "QIIME allows analysis of highthroughput community sequencing data", Nat. Methods, 7, pp.335~336 (2010)
  35. Schloss PD, "Introducing mothur : open-source, platformindependent, community-supported software for describing and comparing microbial communities", Appl. Environ. Microbiol, 75, pp.7537~7541 (2009)
  36. Bulgarelli D, "Structure and function of the bacterial root microbiota in wild and domesticated barley", Cell Host Microbe, 17, pp.392~403 (2015)
  37. Zhou JZ, "Reproducibility and quantitation of amplicon sequencing-based detection", ISME J, 5, pp.1303~1313 (2011)
  38. Edwards J, "Structure, variation, and assembly of the root-associated microbiomes of rice", Proc. Natl. Acad. Sci. USA, 112, pp.E911~E920 (2015)
  39. Minerdi D, "Nitrogen fixation genes in an endosymbiotic Burkholderia strain", Appl. Environ. Microbiol, 67, pp.725~732 (2001)
  40. Griffith JC, "Contrasting bacterial communities in two indigenous Chionochloa(Poaceae)grassland soils in New Zealand", PLoS One, 12, pp.e0179652~ (2017)
  41. Huang SC, "Isolation, identification, and characterization of an aluminum-tolerant bacterium Burkholderia sp. SB1 from an acidic red soil", Pedosphere, 28, pp.905~912 (2018)
  42. Ash C, "Molecular-identification of ribosomal-Rna group 3 Bacilli(Ash, Farrow, Wallbanks And Collins)using a Pcr probe test-proposal for the creation of a new genus Paenibacillus", Anton. Van Leeuwenhoek, 64, pp.253~260 (1993)
  43. Gardener BBM, "Ecology of Bacillus and Paenibacillus spp. in agricultural systems", Phytopathology, 94, pp.1252~1258 (2004)
  44. Montes MJ, "Paenibacillus antarcticus sp nov., a novel psychrotolerant organism from the Antarctic environment", Int. J. Syst. Evol. Microbiol., 54, pp.1521~1526 (2004)
  45. Jie OY, "Paenibacillus thiaminolyticus : A new cause of human infection, inducing bacteremia in a patient on hemodialysis", Ann. Clin. Lab. Sci, 38, pp.393~400 (2008)
  46. Berge O, "Paenibacillus graminis sp nov and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food", Int. J. Syst. Evol. Microbiol., 52, pp.607~616 (2002)
  47. Valverde A, "Paenibacillus castaneae sp nov., isolated from the phyllosphere of Castanea sativa Miller", Int. J. Syst. Evol. Microbiol., 58, pp.2560~2564 (2008)
  48. Achouak W, "Pseudomonas brassicacearum sp nov and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis thaliana", Int. J. Syst. Evol. Microbiol., 50, pp.9~18 (2000)
  49. Belimov AA, "Pseudomonas brassicacearum strain Am3 containing 1-aminocyclopropane-1-carboxylate deaminase can show both pathogenic and growth-promoting properties in its interaction with tomato", J. Exp. Bot, 58, pp.1485~1495 (2007)
  50. Busse HJ, "Review of the taxonomy of the genus Arthrobacter, emendation of the genus Arthrobacter sensu lato, proposal to reclassify selected species of the genus Arthrobacter in the novel genera Glutamicibacter gen. nov., Paeniglutamicibacter gen. nov., Pseudoglutamicibacter gen. nov., Paenarthrobacter gen. nov and Pseudarthrobacter gen. nov., and emended description of Arthrobacter roseus", Int. J. Syst. Evol. Microbiol., 66, pp.9~37 (2016)
  51. Yan Y, "Functional traits dominate the diversity-related selection of bacterial communities in the rhizosphere", ISME J, 11, pp.56~66 (2017)
  52. Apprill A, "Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton", Aquat. Microb. Ecol, 75, pp.129~137 (2015)
  53. Parada AE, "Every base matters : assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples", Environ. Microbiol, 18, pp.1403~1414 (2016)
  54. Walters W, "Improved bacterial 16S rRNA gene(V4 and V4-5)and fungal internal transcribed spacer marker gene primers for microbial community surveys", mSystems, 22, pp.e00009~e00015 (2016)
  55. Snijders AM, "Influence of early life exposure, host genetics and diet on the mouse gut microbiome and metabolome", Nat. Microbiol, 2, pp.16221~ (2017)

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