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柴梗半夏湯이 LPS로 유발된 급성 폐손상에 대한 영향

Effects of Shigyungbanha-Tang on the Lipopolysaccharide-Induced Acute Lung Injury in Mice

김기태|고흥
한의병리학회 (The Society of Pathology in Korean Medicine)|2009.12|동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)|제23권 제6호|pp. 1349-1357

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참고문헌

키워드

IL-6TNF-αIL-1βRANTESlipopolysaccharideALITARCShigyungbanha-tang
주제분류한의학

초록

This study was performed to investigate the effects of Shigyungbanha-tang(SGT) on the lipopolysaccharide(LPS) induced acute lung injury(ALI) in mice. 1 and 24 h before LPS intratracheal instillation , control group was taken distilled water orally. Treated groups was taken each concentrate SGT(2.5 g/㎏, 6.7 g/㎏) by orally as same times. Normal group was not instilled with LPS and was taken distilled water. 24 h after LPS intratracheal instillation, lung histiology was performed in inflated-fixed lungs in 3 mice of each groups. The other mice of each groups, bronchoalveolar lavege fluids(BALF) was obtained to measure proinflammatory cytokines(TNF-α, IL-1β, IL-6) and blood sample was obtained to measure white blood cell(WBC). In vitro, the effect of SGT(100 ug/㎖, 500 ug/㎖, 1000 ug/㎖) on the release of RANTES, TARC induced by TNF-α and IL-4 in human aveolar epithelial cell(A549) was examined. Histopathologically, SGT prevented LPS-induced lung injury. SGT decreased protein, TNF-α, IL-1β and IL-6 according to concentrations. In vitro, 500 ug/㎖, 1000 ug/㎖ concentrate SGT suppressed the expression of RANTES and TARC on A549 cells. On the basis of these results, SGT had a markedly anti-inflammatory effect in a clinically relevant model of ALI. Nevertheless, further investigations are required to determine the potential clinical usefulness of SGT in the adjunctive therapy of ALI.

Abstract

This study was performed to investigate the effects of Shigyungbanha-tang(SGT) on the lipopolysaccharide(LPS) induced acute lung injury(ALI) in mice. 1 and 24 h before LPS intratracheal instillation , control group was taken distilled water orally. Treated groups was taken each concentrate SGT(2.5 g/㎏, 6.7 g/㎏) by orally as same times. Normal group was not instilled with LPS and was taken distilled water. 24 h after LPS intratracheal instillation, lung histiology was performed in inflated-fixed lungs in 3 mice of each groups. The other mice of each groups, bronchoalveolar lavege fluids(BALF) was obtained to measure proinflammatory cytokines(TNF-α, IL-1β, IL-6) and blood sample was obtained to measure white blood cell(WBC). In vitro, the effect of SGT(100 ug/㎖, 500 ug/㎖, 1000 ug/㎖) on the release of RANTES, TARC induced by TNF-α and IL-4 in human aveolar epithelial cell(A549) was examined. Histopathologically, SGT prevented LPS-induced lung injury. SGT decreased protein, TNF-α, IL-1β and IL-6 according to concentrations. In vitro, 500 ug/㎖, 1000 ug/㎖ concentrate SGT suppressed the expression of RANTES and TARC on A549 cells. On the basis of these results, SGT had a markedly anti-inflammatory effect in a clinically relevant model of ALI. Nevertheless, further investigations are required to determine the potential clinical usefulness of SGT in the adjunctive therapy of ALI.

참고문헌 (34)

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