경기지역 육계의 콕시듐 감염실태 조사
Prevalence of Eimeria infection in commercial broilers in Gyeonggi province, Korea
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Protozoan parasite, the genus Eimeria, causes an intestinal disease, coccidiosis, in young broilers. Coccidiosis induces significant economic loss in poultry production. This study was designed to identify the prevalence of Eimeria spp. in broilers in Gyeonggi province, Korea. Fecal samples from broilers at 94 farms were collected in two chicken slaughterhouses between March and June 2015. Eimeria in-fection was determined by microscopic examination and PCR using ITS-1 region. The prevalence of Eimeria was 58.5% (55 of 94). E. acervulina was identified in 96.4% of samples, E. tenella in 81.8%, E. maxima in 12.7%, E. praecox in 12.7%, E. brunetti in 5.5%, E. necatrix in 1.8%, and E. mitis in 1.8%. Body weight (BW) of broilers infected with both E. tenella and E. acervulina (mean=1.53±0.19B kg) was significantly lower than broilers with E. acervulina positive/E. tenalla negative (mean=1.74±0.07A kg) or Eimeria negative (mean=1.65±0.15A kg) (Duncan’s multiple range test, P＜0.01). High preva-lence of Eimeria tenella and the impact on the broiler body weight shows the importance of this proto-zoa in broiler industry. Development of the periodic monitoring strategy and systemic management for the purpose of the prevention/eradication of Eimeria infection among broilers is required.
Abstract
Protozoan parasite, the genus Eimeria, causes an intestinal disease, coccidiosis, in young broilers. Coccidiosis induces significant economic loss in poultry production. This study was designed to identify the prevalence of Eimeria spp. in broilers in Gyeonggi province, Korea. Fecal samples from broilers at 94 farms were collected in two chicken slaughterhouses between March and June 2015. Eimeria in-fection was determined by microscopic examination and PCR using ITS-1 region. The prevalence of Eimeria was 58.5% (55 of 94). E. acervulina was identified in 96.4% of samples, E. tenella in 81.8%, E. maxima in 12.7%, E. praecox in 12.7%, E. brunetti in 5.5%, E. necatrix in 1.8%, and E. mitis in 1.8%. Body weight (BW) of broilers infected with both E. tenella and E. acervulina (mean=1.53±0.19B kg) was significantly lower than broilers with E. acervulina positive/E. tenalla negative (mean=1.74±0.07A kg) or Eimeria negative (mean=1.65±0.15A kg) (Duncan’s multiple range test, P＜0.01). High preva-lence of Eimeria tenella and the impact on the broiler body weight shows the importance of this proto-zoa in broiler industry. Development of the periodic monitoring strategy and systemic management for the purpose of the prevention/eradication of Eimeria infection among broilers is required.
참고문헌 (27)
- Ayaz M, "Prevalence of coccidiosis in broiler chickens in Faisalabad", Pakistan Pak Vet J, 23, pp.51~52 (2003)
- Bachaya HA, "Existence of coccidiosis and associated risk factors in broiler chickens in Southern Punjab", Pakistan Pak Vet J, 35, pp.81~84 (2015)
- Bachaya HA, "Predominance and detection of different Eimeria species causing coccidiosis in layer chickens", J Anim Plant Sci, 22, pp.597~600 (2012)
- Daszak P, "Zoite Migration during Eimeria tenella Infection:Parasite Adaptation to Host", Defences Parasitology Today, 15, pp.67~72 (1999)
- Del Cacho E, "Expression of anti-apoptotic factors in cells parasitized by second-generation schizonts of Eimeria tenella and Eimeria necatrix", Vet Parasitol, 125, pp.287~300 (2004)
- Dunn A, "Factors affecting the reliability of the McMaster technique", J Helminthol, 60, pp.260~262 (1986)
- FitzCoy SH, "Pathogenicity and control of Eimeria mitis infections in broiler chickens", Avain Dis, 36, pp.44~48 (1992)
- Gordon HM, "A new technique for counting nematode eggs in sheep faeces", J Coun Sci Ind Res, 12, pp.50~52 (1939)
- Hamidinejat H, "Characterization of Eimeria Species in Commercial Broilers by PCR Based on ITS1 Regions of rDNA Iran", J Parasitol, 5, pp.48~54 (2010)
- Haug A, "Coccidial infections in commercial broilers: epidemiological aspects and comparison of Eimeria species identification by morphometric and polymerase chain reaction techniques", Avian Pathol, 37, pp.161~170 (2008)
- Haug A, "A simplified protocol for molecular identification of Eimeria species in field samples", Vet Parasitol, 146, pp.35~45 (2007)
- Haug A, "Counting coccidial oocysts in chicken faeces: a comparative study of a standard McMaster technique and a new rapid method", Vet Parasitol, 136, pp.233~242 (2006)
- Khan MQ, "Eimeriosis in poultry of Rawalpindi", Islamabad area, 2006, pp.26, 85~87
- Lee BH, "Prevalence and cross-immunity of Eimeria species on Korean chicken farms", J Vet Med Sci, 72, pp.985~989 (2010)
- Lew AE, "Inter- and intra-strain variation and PCR detection of the internal transcribed spacer 1 (ITS-1) sequences of Australian isolates of Eimeria species from chickens", Vet Parasitol, 112, pp.33~50 (2003)
- Mattiello R, "Eimeria brunetti and E. necatrix in chickens of Argentina and confirmation of seven species of Eimeria", Avian Dis, 44, pp.711~714 (2000)
- Mouafo AN, "Observation of sutures in the oocyst wall of Eimeria tenella (Apicomplexa)", Parasitol Res, 86, pp.1015~1017 (2000)
- MyungJo Y, "The comparative analysis of infection pattern and oocyst output in Eimeria tenella, E. maxima and E. acervulina in young broiler chicken", Veterinary World (2014)
- Reid WM, "Coccidia and Coccidiosis" (1970), Akaemine Kiado
- Saif YM, "Diseases of Poultry", pp.1070~1075 (2008), Blackwell Publishing
- Schnitzler BE, "Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic Eimeria species of the chicken", Avian Pathol, 27, pp.490~497 (1998)
- Schnitzler BE, "PCR identication of chicken Eimeria: a simplied read-out", Avian Pathol, 28, pp.89~93 (1999)
- Shirley MW, "The biology of avian Eimeria with an emphasis on their control by vaccination", Adv Parasitol, 60, pp.285~330 (2005)
- Su YC, "Differential diagnosis of five avian Eimeria species by polymerase chain reaction using primers derived from the internal transcribed spacer 1 (ITS-1) sequence", Vet Parasitol, 117, pp.221~227 (2003)
- Thebo P, "Identification of seven Eimeria species in Swedish domestic fowl", Avian Pathology, 27, pp.613~617 (1998)
- Waldenstedt L, "Sporulation of Eimeria maxima oocysts in litter with different moisture contents", Poult Sci, 80, pp.1412~1415 (2001)
- Williams RB, "Epidemiological aspects of the use of live anticoccidial vaccines for chickens", Int J Parasitol, 28, pp.1089~1098 (1998)