Recombinant DNA and Protein Vaccines for Foot-and-mouth Disease Induce Humoral and Cellular Immune Responses in Mice
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Foot-and-mouth disease virus (FMDV) is a small single- stranded RNA virus which belongs to the family Picornaviridae, genus Apthovirus. It is a principal cause of FMD which is highly contagious in livestock. In a wild type virus infection, infected animals usually elicit antibodies against structural and non-structural protein of FMDV. A structural protein, VP1, is involved in neutralization of virus particle, and has both B and T cell epitopes. A RNA-dependent RNA polymerase, 3D, is highly conserved among other serotypes and strongly immunogenic, therefore, we selected VP1 and 3D as vaccine targets. VP1 and 3D genes were codon-optimized to enhance protein expression level and cloned into mammalian expression vector. To produce recombinant protein, VP1 and 3D genes were also cloned into pET vector. The VP1 and 3D DNA or proteins were co-immunized into 5 weeks old BALB/C mice. Antigen-specific serum antibody (Ab) responses were detected by Ab ELISA. Cellular immune response against VP1 and 3D was confirmed by ELISpot assay. The results showed that all DNA- and protein-immunized groups induced cellular immune responses, suggesting that both DNA and recombinant protein vaccine administration efficiently induced Ag-specific humoral and cellular immune responses.
Abstract
Foot-and-mouth disease virus (FMDV) is a small single- stranded RNA virus which belongs to the family Picornaviridae, genus Apthovirus. It is a principal cause of FMD which is highly contagious in livestock. In a wild type virus infection, infected animals usually elicit antibodies against structural and non-structural protein of FMDV. A structural protein, VP1, is involved in neutralization of virus particle, and has both B and T cell epitopes. A RNA-dependent RNA polymerase, 3D, is highly conserved among other serotypes and strongly immunogenic, therefore, we selected VP1 and 3D as vaccine targets. VP1 and 3D genes were codon-optimized to enhance protein expression level and cloned into mammalian expression vector. To produce recombinant protein, VP1 and 3D genes were also cloned into pET vector. The VP1 and 3D DNA or proteins were co-immunized into 5 weeks old BALB/C mice. Antigen-specific serum antibody (Ab) responses were detected by Ab ELISA. Cellular immune response against VP1 and 3D was confirmed by ELISpot assay. The results showed that all DNA- and protein-immunized groups induced cellular immune responses, suggesting that both DNA and recombinant protein vaccine administration efficiently induced Ag-specific humoral and cellular immune responses.
참고문헌 (20)
- Balamurugan V, "Past and present vaccine development strategies for the control of foot-and-mouth disease", Acta Virol, 48, pp.201~214 (2004)
- Bautista EM, "Constitutive expression of alpha interferon by skin dendritic cells confers resistance to infection by foot-and-mouth disease virus", J Virol, 79, pp.4838~4847 (2005)
- Borrego B, "DNA vaccines expressing B and T cell epitopes can protect mice from FMDV infection in the absence of specific humoral responses", Vaccine, 24, pp.3889~3899 (2006)
- Cedillo-Barron L, "Induction of a protective response in swine vaccinated with DNA encoding foot-and-mouth disease virus empty capsid proteins and the 3D RNA polymerase", J Gen Virol, 82, pp.1713~1724 (2001)
- Cedillo-Barron L, "Immunogenicity of plasmids encoding T and B cell epitopes of foot-and-mouth disease virus (FMDV) in swine", Vaccine, 21, pp.4261~4269 (2003)
- Chinsangaram J, "Antibody response in mice inoculated with DNA expressing foot-and-mouth disease virus capsid proteins", J Virol, 72, pp.4454~4457 (1998)
- Collen T, "Heterotypic recognition of recombinant FMDV proteins by bovine T-cells: the polymerase (P3Dpol) as an immunodominant T-cell immunogen", Virus Res, 56, pp.125~133 (1998)
- Collen T, "A T cell epitope in VP1 of foot-and-mouth disease virus is immunodominant for vaccinated cattle", J Immunol, 146, pp.749~755 (1991)
- Eriksson E, "In vivo gene transfer to skin and wound by microseeding", J Surg Res, 78, pp.85~91 (1998)
- Kim SA, "DNA vaccination against foot-and-mouth disease via electroporation:study of molecular approaches for enhancing VP1 antigenicity", J Gene Med, 8, pp.1182~1191 (2006)
- Knowles NJ, "Pandemic strain of foot-and-mouth disease virus serotype O", Emerg Infect Dis, 11, pp.1887~1893 (2005)
- Jong Hyeon Park, "Enhanced immune response with foot and mouth disease virus VP1 and interleukin-1 fusion genes", Journal of Veterinary Science, 7(3), pp.257~262 (2006), 대한수의학회
- Patil PK, "Integrity of GH-loop of foot-and-mouth disease virus during virus inactivation: detection by epitope specific antibodies", Vaccine, 20, pp.1163~1168 (2002)
- Raz E, "Intradermal gene immunization: the possible role of DNA uptake in the induction of cellular immunity to viruses", Proc Natl Acad Sci U S A, 91, pp.9519~9523 (1994)
- Sanz-Parra A, "Infection with foot-andmouth disease virus results in a rapid reduction of MHC class I surface expression", J Gen Virol, 79, pp.433~436 (1998)
- van Lierop MJ, "Sequences derived from the highly antigenic VP1 region 140 to 160 of foot-and-mouth disease virus do not prime for a bovine T-cell response against intact virus", J Virol, 69, pp.4511~4514 (1995)
- Wang JH, "Induction of immunity in swine by purified recombinant VP1 of foot-and-mouth disease virus", Vaccine, 21, pp.3721~3729 (2003)
- Yang JS, "Induction of potent Th1-type immune responses from a novel DNA vaccine for West Nile virus New York isolate (WNV-NY1999)", J Infect Dis, 184, pp.809~816 (2001)
- Yang NS, "Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot-and-mouth disease virus", J Gene Med, 7, pp.708~717 (2005)
- Zhang Q, "A recombinant fusion protein and DNA vaccines against foot-and-mouth disease virus type Asia 1 infection in guinea pigs", Acta Virol, 47, pp.237~243 (2003)