적응진화를 활용한 cellobiose와 xylose 동시발효 Pichia stipitis의 개발
Development of Pichia stipitis Co-fermenting Cellobiose and Xylose Through Adaptive Evolution
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섬유소계 바이오매스로부터 바이오 연료 등과 같은 유용한 물질을 생산하기 위해서는 바이오매스로부터 유래하는혼합당을 효과적으로 대사할 수 있는 균주의 개발이 필수적이다. 본 연구에서는 xylose를 대사가 가능한 효모인 P. stipitis를 적응진화하여 cellobiose 대사효율이 향상되고cellobiose와 xylose를 동시에 대사할 수 있는 균주를 개발하고자 하였다. 총 10회의 계대배양을 통해 얻어진 진화된 P. stipitis 돌연변이 균주는 모균주에 비해 6배 이상 증가된cellobiose 대사속도를 나타내었으며 ethanol 생산수율을 0 에서 0.4 (g ethanol/g cellobiose)로 향상시켰다. 아울러 본실험에서 개발한 돌연변이 균주는 cellobiose와 xylose 혼합당 조건에서 모균주에 비해 2배 가까이 향상된 ethanol 생산 및 생산속도를 나타내었다.
Abstract
Production of biofuels and value-added materials from cellulosic biomass requires the development of a microbial strain capable of efficiently fermenting mixed sugars. In this study, the natural xylose fermenting yeast, Pichia stipitis, was evolved to simultaneously ferment cellobiose and xylose. Serial subcultures of wild-type P. stipitis in 20 g/l cellobiose were performed to increase the rate of cellobiose consumption. A total of ten rounds of the serial subculture led to the isolation of an evolved strain fermenting cellobiose significantly faster than the parental strain. The evolved strain displayed enhanced ethanol yield from 0 to 0.4 g ethanol/g cellobiose. The evolved P. stipitis simultaneously fermented cellobiose and xylose in batch fermentation. The genetic information of our evolved P. stipitis would be valuable in the development of a microbial host for the production of biofuels and biomaterials from cellulosic biomass.
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