The Effect of Cold Ischemic Time on Immunohistochemical Evaluation of Invasive Ductal Carcinoma
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Purpose: Immunohistochemical (IHC) evaluation is important for the management of breast cancer. Cold ischemic time (CIT) may result in inaccurate IHC results. The aim of this study was to investigate the effect of CIT on IHC results of invasive ductal carcinoma (IDC). Methods: We retrospectively reviewed the records of 316 patients diagnosed with IDC from February 2013 to June 2013 at a single hospital in Korea. The clinicopathological characteristics and IHC positivity for the estrogen receptor (ER), progesterone receptor (PR), Ki-67, and human epidermal growth factor receptor 2 (HER2) were compared between specimens with a CIT of ≤3 hours (short CIT) and those with a CIT of >12 hours (long CIT). Results: Among the 316 patients, 227 had a CIT of ≤3 hours and 89 had a CIT of >12 hours. No difference in positivity for ER (p=0.734), PR (p=0.870), and HER2 (p=0.830) was observed between the two groups. In ER-positive specimens, the mean percentage value was higher in the long CIT group than in the short CIT group (81.3±20.8% vs. 76.0±26.3%, respectively; p=0.021). The mean Ki-67 value was higher in the long CIT group compared to the short CIT group (9.7±14.6% vs. 7.0±10.8%, respectively; p=0.047). Conclusion: CIT did not affect the positivity of ER, PR, HER2, and Ki-67 as determined by IHC when stored at 4°C overnight. However, specimens with a long CIT had a higher ER percentage value in ER-positive tumors and a higher Ki-67 value in all specimens compared to those with a short CIT. These differences should be considered in the interpretation of IHC results in specimens with a long CIT.
Abstract
Purpose: Immunohistochemical (IHC) evaluation is important for the management of breast cancer. Cold ischemic time (CIT) may result in inaccurate IHC results. The aim of this study was to investigate the effect of CIT on IHC results of invasive ductal carcinoma (IDC). Methods: We retrospectively reviewed the records of 316 patients diagnosed with IDC from February 2013 to June 2013 at a single hospital in Korea. The clinicopathological characteristics and IHC positivity for the estrogen receptor (ER), progesterone receptor (PR), Ki-67, and human epidermal growth factor receptor 2 (HER2) were compared between specimens with a CIT of ≤3 hours (short CIT) and those with a CIT of >12 hours (long CIT). Results: Among the 316 patients, 227 had a CIT of ≤3 hours and 89 had a CIT of >12 hours. No difference in positivity for ER (p=0.734), PR (p=0.870), and HER2 (p=0.830) was observed between the two groups. In ER-positive specimens, the mean percentage value was higher in the long CIT group than in the short CIT group (81.3±20.8% vs. 76.0±26.3%, respectively; p=0.021). The mean Ki-67 value was higher in the long CIT group compared to the short CIT group (9.7±14.6% vs. 7.0±10.8%, respectively; p=0.047). Conclusion: CIT did not affect the positivity of ER, PR, HER2, and Ki-67 as determined by IHC when stored at 4°C overnight. However, specimens with a long CIT had a higher ER percentage value in ER-positive tumors and a higher Ki-67 value in all specimens compared to those with a short CIT. These differences should be considered in the interpretation of IHC results in specimens with a long CIT.
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