젖소 유방염에서 분리한 Pseudomonas spp.의 분포 및 항생제 내성 비교
Comparison on prevalence and antimicrobial resistance of Pseudomonas spp. isolated from bovine mastitis milk in South Korea
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study was aimed to investigate the prevalence and antimicrobial resistance of Pseudomonas spp. isolated from bovine mastitis milk samples. A total of 50 (4.9%) Pseudomonas spp. was isolated from 1,023 samples, those collected between 2018 and 2021, derived from 110 dairy farms. The prevalence of the identified species of Pseudomonas isolates was as follows; P. aeruginosa (70.0%), P. fluorescens (14.0%), P. putida (10.0%), P. fragi (4.0%), and P. chlororaphis (2.0%). Most of somatic cell counts in the quarter milk carrying Pseudomonas spp. were less than 3,000,000 cell/ml (90.0%). The iso-lates of Pseudomonas spp. showed high susceptibility to cefepime (98.0%), ciprofloxacin (98.0%), ceftazidime (96.0%), and colistin (96.0%). The rate of antibiotic resistance in the isolates was highest to ceftiofur (92.0%), followed by the resistance rate to chloramphenicol (86.0%) and trimethoprim/sulphamethoxazole (80.0%). In addition, there is a remarkable difference in antimicrobial resistance pattern among Pseudomonas species. P. aeruginosa and P. putida showed a similar resistance pattern, whereas P. fluorescens showed exceptionally lower resistance to trimethoprim/sulphamethoxazole and chloramphenicol than that of the other species. This study showed that prevalence of Pseudo-monas spp. other than P. aeruginosa were 30.0% in bovine mastitis milk, and the occurrence rate of antibiotic resistance were similar or higher level, compared with the previous reports on the mastitis-derived Pseudomonas spp. isolated in Korea.
Abstract
This study was aimed to investigate the prevalence and antimicrobial resistance of Pseudomonas spp. isolated from bovine mastitis milk samples. A total of 50 (4.9%) Pseudomonas spp. was isolated from 1,023 samples, those collected between 2018 and 2021, derived from 110 dairy farms. The prevalence of the identified species of Pseudomonas isolates was as follows; P. aeruginosa (70.0%), P. fluorescens (14.0%), P. putida (10.0%), P. fragi (4.0%), and P. chlororaphis (2.0%). Most of somatic cell counts in the quarter milk carrying Pseudomonas spp. were less than 3,000,000 cell/ml (90.0%). The iso-lates of Pseudomonas spp. showed high susceptibility to cefepime (98.0%), ciprofloxacin (98.0%), ceftazidime (96.0%), and colistin (96.0%). The rate of antibiotic resistance in the isolates was highest to ceftiofur (92.0%), followed by the resistance rate to chloramphenicol (86.0%) and trimethoprim/sulphamethoxazole (80.0%). In addition, there is a remarkable difference in antimicrobial resistance pattern among Pseudomonas species. P. aeruginosa and P. putida showed a similar resistance pattern, whereas P. fluorescens showed exceptionally lower resistance to trimethoprim/sulphamethoxazole and chloramphenicol than that of the other species. This study showed that prevalence of Pseudo-monas spp. other than P. aeruginosa were 30.0% in bovine mastitis milk, and the occurrence rate of antibiotic resistance were similar or higher level, compared with the previous reports on the mastitis-derived Pseudomonas spp. isolated in Korea.
참고문헌 (22)
- Banerjee S, "Detection and characterization of pathogenic Pseudomonas aeruginosa from bovine subclinical mastitis in West Bengal, India", Vet World, 10, pp.738~ (2017)
- Bogni C, "War against mastitis: Current concepts on controlling bovine mastitis pathogens, Science against microbial pathogens: Communicating current research and technological advances", 483~494 (2011)
- Cheng Wei Nee, "Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments", Animal Bioscience, 33(11), pp.1699~1713 (2020), 아세아·태평양축산학회[DOI]
- Performance standards for antimicrobial susceptibility testing, "CLSI", CLSI, ~ (2019)
- Erskine R, "Trends in antibacterial susceptibility of mastitis pathogens during a seven-year period", J Dairy sci, 85, pp.1111~1118 (2002)
- Gomes F, "Control of bovine mastitis : old and recent therapeutic approaches", Curr Microbiol, 72, pp.377~382 (2016)
- Halasa T, "Economic effects of bovine mastitis and mastitis management : a review", Vet Q, 29, pp.18~31 (2007)
- Kang HJ, "Identification and antimicrobial susceptibility of microorganisms isolated from bovine mastitic milk", Korean J Vet Res, 41, pp.511~521 (2001)
- Kawai K, "Control of Pseudomonas mastitis on a large dairy farm by using slightly acidic electrolyzed water", Anim Sci J, 88, pp.1601~1605 (2017)
- 김성은, "경남지역의 체세포수 문제 목장에서의 젖소 유방염 관리실태 및 발생양상 조사", 한국동물위생학회지, 34(4), pp.379~388 (2011), 한국동물위생학회
- 문진산, "젖소 유방염으로부터 분리한 그람음성균의 분포 및 항생제 감수성", 대한수의학회지, 47(1), pp.67~75 (2007), 대한수의학회
- 문진산, "젖소 유방염 유즙에서 분리한 Streptococcus uberis의 항생제 감수성 및 유전학적 특성", 대한수의학회지, 47(1), pp.33~41 (2007), 대한수의학회
- Nam HM, "Prevalence and antimicrobial susceptibility of gram-negative bacteria isolated from bovine mastitis between 2003 and 2008 in Korea", J Dairy Sci, 92, pp.2020~2026 (2009)
- 남향미, "Culture results from quarter milk samples submitted to veterinary diagnostic laboratories during January~November 2012 in Korea", 예방수의학회지, 37(3), pp.111~119 (2013), 한국예방수의학회
- Nam HM, "Antimicrobial resistance of enterococci isolated from mastitic bovine milk samples in Korea", Zoonoses Public Health, 57, pp.e59~e64 (2010)
- NMC, "Laboratory Handbook on Bovine Mastitis" (2017), NMC
- Ohnishi M, "Antimicrobial susceptibilities and bacteriological characteristics of bovine Pseudomonas aeruginosa and Serratia marcescens isolates from mastitis", Vet Microbiol, 154, pp.202~207 (2011)
- Park HR, "Characterisation of Pseudomonas aeruginosa related to bovine mastitis", Acta Vet Hung, 62, pp.1~12 (2014)
- Saidi R, "Subclinical mastitis in cattle in Algeria: frequency of occurrence and bacteriological isolates", J S Afr Vet Assoc, 84, pp.E1~E5 (2013)
- Schauer B, "Tracing Mastitis Pathogens—Epidemiological Investigations of a Pseudomonas aeruginosa Mastitis Outbreak in an Austrian Dairy Herd", Animals, 11, pp.279~ (2021)
- Smith KL, "Environmental mastitis", Vet Clin North Am Food Anim Pract, 9, pp.489~498 (1993)
- Suleiman T, "Prevalence of bovine subclinical mastitis and antibiotic susceptibility patterns of major mastitis pathogens isolated in Unguja island of Zanzibar, Tanzania", Trop Anim Health Prod, 50, pp.259~266 (2018)