A 24 kDa Excretory-Secretory Protein of Anisakis simplex Larvae Could Elicit Allergic Airway Inflammation in Mice
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We have reported that a 24 kDa protein (22U homologous; As22U) of Anisakis simplex larvae could elicit several Th2-related chemokine gene expressions in the intestinal epithelial cell line which means that As22U may play a role as an allergen. In order to determine the contribution of As22U to allergic reactions, we treated mice with 6 times intra-nasal application of recombinant As22U (rAs22U). In the group challenged with rAs22U and ovalbumin (OVA), the number of eosinophils in the bronchial alveolar lavage fluid (BALF) was significantly increased, as compared to the group receiving only OVA. In addition, mice treated with rAs22U and OVA showed significantly increased airway hyperresponsiveness. Thus, severe inflammation around the airway and immune cell recruitment was observed in mice treated with rAs22U plus OVA. The levels of IL-4, IL-5, and IL-13 cytokines in the BALF increased significantly after treatment with rAs22U and OVA. Similarly, the levels of anti-OVA specific IgE and IgG1 increased in mice treated with rAs22U and OVA, compared to those treated only with OVA. The Gro-α (CXCL1) gene expression in mouse lung epithelial cells increased instantly after treatment with rAs22U, and allergy-specific chemokines eotaxin (CCL11) and thymus-and-activation-regulated-chemokine (CCL17) gene expressions significantly increased at 6 hr after treatment. In conclusion, rAs22U may induce airway allergic inflammation, as the result of enhanced Th2 and Th17 responses.
Abstract
We have reported that a 24 kDa protein (22U homologous; As22U) of Anisakis simplex larvae could elicit several Th2-related chemokine gene expressions in the intestinal epithelial cell line which means that As22U may play a role as an allergen. In order to determine the contribution of As22U to allergic reactions, we treated mice with 6 times intra-nasal application of recombinant As22U (rAs22U). In the group challenged with rAs22U and ovalbumin (OVA), the number of eosinophils in the bronchial alveolar lavage fluid (BALF) was significantly increased, as compared to the group receiving only OVA. In addition, mice treated with rAs22U and OVA showed significantly increased airway hyperresponsiveness. Thus, severe inflammation around the airway and immune cell recruitment was observed in mice treated with rAs22U plus OVA. The levels of IL-4, IL-5, and IL-13 cytokines in the BALF increased significantly after treatment with rAs22U and OVA. Similarly, the levels of anti-OVA specific IgE and IgG1 increased in mice treated with rAs22U and OVA, compared to those treated only with OVA. The Gro-α (CXCL1) gene expression in mouse lung epithelial cells increased instantly after treatment with rAs22U, and allergy-specific chemokines eotaxin (CCL11) and thymus-and-activation-regulated-chemokine (CCL17) gene expressions significantly increased at 6 hr after treatment. In conclusion, rAs22U may induce airway allergic inflammation, as the result of enhanced Th2 and Th17 responses.
참고문헌 (45)
- Moreno-Ancillo A, "Allergic reactions to Anisakis simplex parasitizing seafood", Ann Allergy Asthma Immunol, 79, pp.246~250 (1997)
- Nieuwenhuizen N, "Exposure to the fish parasite Anisakis causes allergic airway hyperreactivity and dermatitis", J Allergy Clin Immunol, 117, pp.1098~1105 (2006)
- Armentia A, "Occupational asthma by Anisakis simplex", J Allergy Clin Immunol, 102, pp.831~834 (1998)
- Barbuzza O, "Pro tein contact dermatitis and allergic asthma caused by Anisakis simplex", Contact Dermatitis, 60, pp.239~240 (2009)
- Jeebhay MF, "Occupational seafood allergy: A review", Occup Environ Med, 58, pp.553~562 (2001)
- Nieuwenhuizen N, "Differential requirements for interleukin (IL)-4 and IL-13 in protein contact dermatitis induced by Anisakis", Allergy, 64, pp.1309~1318 (2009)
- Moneo I, "Isolation and characterization of a major allergen from the fish parasite Anisakis simplex", J Allergy Clin Immunol, 106, pp.177~182 (2000)
- Perez-Perez J, "Molecular cloning of paramyosin, a new allergen of Anisakis simplex", Int Arch Allergy Immunol, 123, pp.120~129 (2000)
- Asturias JA, "Cloning and high level expression in Escherichia coli of an Anisakis simplex tropomyosin isoform", Mol Biochem Parasitol, 108, pp.263~267 (2000)
- Rodriguez-Mahillo AI, "Cloning and characterisation of the Anisakis simplex allergen Ani s 4 as a cysteine-protease inhibitor", Int J Parasitol, 37, pp.907~917 (2007)
- Kobayashi Y, "Molecular cloning and expression of two new allergens from Anisakis simplex", Parasitol Res, 100, pp.1233~1241 (2007)
- Kobayashi Y, "Purification and cDNA cloning of a new heat-stable allergen from Anisakis simplex", Mol Biochem Parasitol, 155, pp.138~145 (2007)
- Rodriguez-Perez R, "Cloning and expression of Ani s 9, a new Anisakis simplex allergen", Mol Biochem Parasitol, 159, pp.92~97 (2008)
- Caballero ML, "Ani s 10, a new Anisakis simplex allergen: Cloning and heterologous expression", Parasitol Int, 60, pp.209~212 (2011)
- Kobayashi Y, "Identification of novel three allergens from Anisakis simplex by chemiluminescent immunoscreening of an expression cDNA library", Parasitol Int, 60, pp.144~150 (2011)
- Rodriguez-Mahillo AI, "Identification and allergenic characterisation of a new isoform of the A .simplex allergen Ani s 4", Mol Biochem Parasitol, 160, pp.152~156 (2008)
- Park JS, "Identification of 24 kDa excretory secretory protein of Anisakis simplex", Experimental Parasitology, 130, pp.69~72
- Kouzaki H, "Proteases induce production of thymic stromal lymphopoietin by airway epithelial cells through protease-activated receptor-2", J Immunol, 183, pp.1427~1434 (2009)
- Chou H, "Vacuolar serine protease is a major allergen of Cladosporium cladosporioides", Int Arch Allergy Immunol, 146, pp.277~286 (2008)
- Kiss A, "A new mechanism regulating the initiation of allergic airway inflammation", J Allergy Clin Immunol, 120, pp.334~342 (2007)
- Angkasekwinai P, "Interleukin 25 promotes the initiation of proallergic type 2 responses", J Exp Med, 204, pp.1509~1517 (2007)
- Wang YH, "IL-25 augments type 2 immune responses by enhancing the expansion and functions of TSLP-DC-activated Th2 memory cells", J Exp Med, 204, pp.1837~1847 (2007)
- Trujillo-Vargas CM, "Vaccinations with T-helper type 1 directing adjuvants have different suppressive effects on the development of allergen-induced T-helper type 2 responses", Clin Exp Allergy, 35, pp.1003~1013 (2005)
- Falcone FH, "The innate allergenicity of helminth parasites", Clin Rev Allergy Immunol, 26, pp.61~72 (2004)
- Rook GA, "Review series on helminths, immune modulation and the hygiene hypothesis: the broader implications of the hygiene hypothesis", Immunology, 126, pp.3~11 (2009)
- Osada Y, "Kanazawa T. Schistosoma mansoni infection reduces severity of collagen-induced arthritis via down-regulation of pro-inflammatory mediators", Int J Parasitol, 39, pp.457~464 (2009)
- Pacifico LG, "Schistosoma mansoni antigens modulate experimental allergic asthma in a murine model: A major role for CD4+ CD25+ Foxp3+ T cells independent of interleukin-10", Infect Immun, 77, pp.98~107 (2009)
- Pinelli E, "Infection with the roundworm Toxocara canis leads to exacerbation of experimental allergic airway inflammation", Clin Exp Allergy, 38, pp.649~658 (2008)
- Buijs J, "Toxocara-induced eosinophilic inflammation. Airway function and effect of anti-IL-5", Am J Respir Crit Care Med, 151, pp.873~878 (1995)
- Ramsey W, "Ascaris pneumonitis: case presentation", Iowa Med, 79, pp.484~486 (1989)
- Takeuchi H, "High titre of anti-Ascaris immunoglobulin E associated with bronchial asthma symptoms in 5-year-old rural Bangladeshi children", Clin Exp Allergy, 38, pp.276~282 (2008)
- Sakakibara A, "Visceral larva migrans due to Ascaris suum which presented with eosinophilic pneumonia and multiple intra-hepatic lesions with severe eosinophil infiltration--outbreak in a Japanese area other than Kyushu", Intern Med, 41, pp.574~579 (2002)
- Cho MK, "Parasite excretory-secretory proteins elicit TRIF dependent CXCL1 and IL-6 mediated allergic inflammation", PARASITE IMMUNOLOGY, 32, pp.354~360 (2010), BLACKWELL PUBLISHING
- Marsland BJ, "Secretory products from infective forms of Nippostrongylus brasiliensis induce a rapid allergic airway inflammatory response", Immunol Cell Biol, 83, pp.40~47 (2005)
- Donnelly S, "Proteases in helminth- and allergen- induced inflammatory responses", Chem Immunol Allergy, 90, pp.45~64 (2006)
- Webb DC, "Integrated signals between IL-13, IL-4, and IL-5 regulate airways hyperreactivity", J Immunol, 165, pp.108~113 (2000)
- Sher A, "Ablation of eosinophil and IgE responses with anti-IL-5 or anti-IL-4 antibodies fails to affect immunity against Schistosoma mansoni in the mouse", J Immunol, 145, pp.3911~3916 (1990)
- Ishizaka T, "Immunoglobulin E synthesis in parasite infection", J Allergy Clin Immunol, 58, pp.523~538 (1976)
- Ehigiator HN, "Nippostrongylus brasiliensis stimulates polyclonal type-2 immunoglobulin response by inducing De novo class switch", Infect Immun, 68, pp.4913~4922 (2000)
- Prause O, "Pharmacological modulation of interleukin-17-induced GCP-2-, GRO-alpha- and interleukin-8 release in human bronchial epithelial cells", Eur J Pharmacol, 462, pp.193~198 (2003)
- Kawaguchi M, "Induction of C-X-C chemokines, growth-related oncogene alpha expression, and epithelial cell-derived neutrophil-activating protein-78 by ML-1 (interleukin-17F) involves activation of Raf1-mitogen-activated protein kinase kinase-extracellular signal-regulated kinase 1", 2 pathway, 2003, pp.307, 1213~1220
- Cheung PF, "Molecular mechanisms of cytokine and chemokine release from eosinophils activated by IL-17A, IL-17F, and IL-23: implication for Th17 lymphocytes-mediated allergic inflammation", J Immunol, 180, pp.5625~5635 (2008)
- Shainheit MG, "Dendritic cell IL-23 and IL-1 production in response to schistosome eggs induces Th17 cells in a mouse strain prone to severe immunopathology", J Immunol, 181, pp.8559~8567 (2008)
- Mathew A, "Signal transducer and activator of transcription 6 controls chemokine production and T helper cell type 2 cell trafficking in allergic pulmonary inflammation", Exp Med, 193, pp.1087~1096 (2001)
- Sallusto F, "The role of chemokines and chemokine receptors in T cell priming and Th1", Th2-mediated responses, 1999, pp.84(4), 28~31
