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Expression of vascular endothelial growth factor in oral squamous cell carcinoma

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Objectives: The goal of this study was to determine the correlation of clinicopathological factors and the up-regulation of vascular endothelial growth factor (VEGF) expression in oral squamous cell carcinoma.Materials and Methods: Immunohistochemical staining of VEGF and quantitative real-time polymerase chain reaction (RT-PCR) of VEGF mRNA were performed in 20 specimens from 20 patients with oral squamous cell carcinoma and another 20 specimens from 20 patients with carcinoma in situ as a controlled group.Results: The results were as follows: 1) In immunohistochemical study of poorly differentiated and invasive oral squamous cell carcinoma, high-level staining of VEGF was observed. Significantcorrelation was observed between immunohistochemical VEGF expression and histologic differentia-tion, tumor size of specimens (Pearson correlation analysis, significancer>0.6, P<0.05). 2) In VEGF quantitative RT-PCR analysis, progressive cancer showed more VEGF expression than carcinoma in situ. Paired-samples analysis determined the difference of VEGF mRNA expression level between cancer tissue and carcinoma in situ tissue, between T1 and T2-4 (Student’s t-test, P<0.05).Conclusion: These fndings suggest that up-regulation of VEGF may play a role in the angiogenesis and progression of oral squamous cell carcinoma.

Abstract

Objectives: The goal of this study was to determine the correlation of clinicopathological factors and the up-regulation of vascular endothelial growth factor (VEGF) expression in oral squamous cell carcinoma.Materials and Methods: Immunohistochemical staining of VEGF and quantitative real-time polymerase chain reaction (RT-PCR) of VEGF mRNA were performed in 20 specimens from 20 patients with oral squamous cell carcinoma and another 20 specimens from 20 patients with carcinoma in situ as a controlled group.Results: The results were as follows: 1) In immunohistochemical study of poorly differentiated and invasive oral squamous cell carcinoma, high-level staining of VEGF was observed. Significantcorrelation was observed between immunohistochemical VEGF expression and histologic differentia-tion, tumor size of specimens (Pearson correlation analysis, significancer>0.6, P<0.05). 2) In VEGF quantitative RT-PCR analysis, progressive cancer showed more VEGF expression than carcinoma in situ. Paired-samples analysis determined the difference of VEGF mRNA expression level between cancer tissue and carcinoma in situ tissue, between T1 and T2-4 (Student’s t-test, P<0.05).Conclusion: These fndings suggest that up-regulation of VEGF may play a role in the angiogenesis and progression of oral squamous cell carcinoma.

참고문헌 (35)

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관련 논문AI추천

Expression of vascular endothelial growth factor receptors in tumor and stromal cells of tongue squamous cell carcinoma김규천, 김덕룡, 김욱규, 김종렬, 박봉수, 박봉욱, 변준호, 정인교, 하영술대한구강악안면외과학회 · 대한구강악안면외과학회지2007 · 제33권 제1호Expression of endoglin and podoplanin in early and advanced oral squamous cell carcinoma박영욱, Sang-Woon Lee대한구강악안면외과학회 · 대한구강악안면외과학회지2012 · 제38권 제3호Expression of angiogenin, TGF-β, VEGF, APEX and TNF-α in oral squamous cell carcinoma김경원, 김원재, 이호선대한구강악안면외과학회 · 대한구강악안면외과학회지2006 · 제32권 제1호혈관내피세포성장인자 억제제에 의한 구강편평상피세포암종 세포주의 성장 억제 효과이재훈, 한세진대한구강악안면외과학회 · 대한구강악안면외과학회지2009 · 제35권 제2호구강편평세포암종의 미세혈관 밀도와 악성도간 상관관계에 관한 임상병리학적 연구명훈, 김명진, 이기철, 임성삼대한구강악안면외과학회 · 대한구강악안면외과학회지2002 · 제28권 제2호Expression of VEGF, HGF, IL-6, IL-8, MMP-9, Telomerase in Peripheral Blood of Patients with Head and Neck Squamous Cell Carcinoma노정훈, 왕수건, 이병주, 이진춘, 최진식, 홍대영대한이비인후과학회 · Clinical and Experimental Otorhinolaryngology2009 · 제2권 제4호

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