Overexpression of AtCAF1, CCR4-associated factor 1 homologue in Arabidopsis thaliana, negatively regulates wounding-mediated disease resistance
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The CCR4-CAF1-NOT complex-mediated degradation of mRNA is a fundamental aspect of gene regulation in eukaryotes. We herein examined the role of AtCAF1 in the innate immune and wound responses of plants. Our results showed that overexpression of AtCAF1 significantly downregulated the transcript level of EFR but not FLS2 and BRI1, as well as abolished up-regulated expression pattern of EFR in response to wounding. Consistently, Agrobacteriummediated transient expression of GUS was highly enhanced in the transgenic plants overexpressing AtCAF. Furthermore, JA responsive genes were down-regulated by overexpression of AtCAF, causing the transgenic plants overexpressing AtCAF more susceptible to necrotrophic fungal pathogen, Botrytis cinerea. These results suggest that The CCR4-CAF1-NOT complex-mediated degradation of mRNA negatively regulates wounding-mediated disease resistance in Arabidopsis thaliana.
Abstract
The CCR4-CAF1-NOT complex-mediated degradation of mRNA is a fundamental aspect of gene regulation in eukaryotes. We herein examined the role of AtCAF1 in the innate immune and wound responses of plants. Our results showed that overexpression of AtCAF1 significantly downregulated the transcript level of EFR but not FLS2 and BRI1, as well as abolished up-regulated expression pattern of EFR in response to wounding. Consistently, Agrobacteriummediated transient expression of GUS was highly enhanced in the transgenic plants overexpressing AtCAF. Furthermore, JA responsive genes were down-regulated by overexpression of AtCAF, causing the transgenic plants overexpressing AtCAF more susceptible to necrotrophic fungal pathogen, Botrytis cinerea. These results suggest that The CCR4-CAF1-NOT complex-mediated degradation of mRNA negatively regulates wounding-mediated disease resistance in Arabidopsis thaliana.
참고문헌 (16)
- Chinchilla D, "A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence", Nature, 26(448), pp.497~500 (2007)
- Clough SJ, "Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana", Plant J, 16, pp.735~743 (1998)
- Collart MA, "The eukaryotic CcR4-not complex: a regulatory platform integrating mRNA metabolism with cellular signaling pathways", Prog Nucleic Acid Res Mol Biol, 77, pp.289~322 (2004)
- 프레드릭, "Arabidopsis local resistance to Botrytis cinerea involves salicylic acid and camalexin and requires EDS4 and PAD2, but not SID2, EDS5 or PAD4", PLANT JOURNAL, 35(2), pp.193~205
- Gόmez-Gόmez L, "FLS2: an LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis", Mol Cell, 5, pp.1003~1011 (2000)
- Kim MG, "Two Pseudomonas syringae type III effectors inhibit RIN4-regulated basal defense in Arabidopsis", Cell, 121(5), pp.749~759 (2005), Cell Press
- Leόn J, "Wound signaling in plants", J Exp Bot, 52, pp.1~9 (2001)
- Liang W, "The Arabidopsis homologs of CCR4-associaed factor1 show mRNA deadenylaion activity and play a role in plant defence responses", Cell Res, 19, pp.307~316 (2009)
- Nam J, "Differences in susceptibility of Arabidopsis ecotypes to crown gall disease may result from a deficiency in T-DNA integration", Plant Cell, 9, pp.317~333 (1997)
- Ness TL, "CCR4 is a key modulator of innate immune responses", J Immunol, 177, pp.7531~7539 (2006)
- Ohn T, "CAF1 plays an important role in mRNA deadenylation separate from its contact to CCR4", Nucl Acids Res, 35, pp.3002~3015 (2007)
- Sarowar S, "Capsicum annuum CCR4-associated factor CaCAF1 is necessary for plant development and defence response", PLANT JOURNAL, 51, pp.792~802 (2007), BLACKWELL PUBLISHING
- Seo HM, "Increased expression of OsPT1, a high-affinity phosphate transporter, enhances phosphate acquisition in rice", Biotechnol Lett, 30, pp.1833~1838 (2008)
- Thore S, "X-ray structure and activity of the yeast Pop2 protein: a nuclease subunit of the mRNA deadenylase complex", EMBO reports, 4, pp.1150~1155 (2003)
- Tucker M, "The transcription factor associated Ccr4 and Caf1 proteins are components of the major cytoplasmic mRNA deadenylase in Saccharomyces cerevisiae", Cell, 104, pp.377~386 (2004)
- Zipfel C, "Perception of the bacterial PAMP EF-Tu by the receptor EFR restricts Agrobacterium-mediated transformation", Cell, 125, pp.749~760 (2006)