누룩으로부터 스트레스 내성이 우수한 Pichia farinosa 균주의 분리
Isolation of Stress-tolerant Pichia farinosa from Nuruk
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
A variety of nuruks collected in different areas in Korea were explored to isolate sixty yeast strains that was able to grow at 44℃. MBY/L1569 strain, which showed the highest growth rate, was selected and identified as Pichia farinosa (Millerozyma farinosa). The isolated strain exhibited superior resistance to heat, acid, and alkali compared with those of P. farinosa KCTC27412 as a control strain. The specific growth rate of P. farinosa MBY/L1569 at 46℃ was 0.37 ± 0.05 h-1, and the highest specific growth rate of 0.50 ± 0.02 h-1 was obtained when it was grown at pH 7.0 and 37℃ with 50 g/l (w/v) glucose as the carbon source. Under optimum growth conditions, strain MBY/L1569 produced ethanol 19.66 ± 0.68 g/l from glucose 50 g/l, with an approximate yield of 40%. P. farinosa MBY/L1569 was deposited at the Korean Collection for Type Cultures as pichia farinosa KCTC27753.
Abstract
A variety of nuruks collected in different areas in Korea were explored to isolate sixty yeast strains that was able to grow at 44℃. MBY/L1569 strain, which showed the highest growth rate, was selected and identified as Pichia farinosa (Millerozyma farinosa). The isolated strain exhibited superior resistance to heat, acid, and alkali compared with those of P. farinosa KCTC27412 as a control strain. The specific growth rate of P. farinosa MBY/L1569 at 46℃ was 0.37 ± 0.05 h-1, and the highest specific growth rate of 0.50 ± 0.02 h-1 was obtained when it was grown at pH 7.0 and 37℃ with 50 g/l (w/v) glucose as the carbon source. Under optimum growth conditions, strain MBY/L1569 produced ethanol 19.66 ± 0.68 g/l from glucose 50 g/l, with an approximate yield of 40%. P. farinosa MBY/L1569 was deposited at the Korean Collection for Type Cultures as pichia farinosa KCTC27753.
참고문헌 (25)
- 최성화, "신재생 대안 에너지로서의 셀룰로스 에탄올", Journal of Plant Biotechnology, 34(2), pp.111~118 (2007), 한국식물생명공학회
- 최다혜, "Isolation and Characterization of Saccharomyces cerevisiae from nuruk for Production of Ethanol from Maltose", 한국미생물·생명공학회지, 44(1), pp.34~39 (2016), 한국미생물·생명공학회[DOI]
- 최기욱, "고효율 바이오 에탄올 생산을 위한 당화효소 개발 및 동시당화발효 공정 연구", KSBB Journal, 23(6), pp.499~503 (2008), 한국생물공학회
- 최수정, "산 및 효소 가수분해를 이용한 홍조류로부터 바이오 에탄올 생산", 공업화학, 23(3), pp.279~283 (2012), 한국공업화학회[DOI]
- 이민웅, "Effect of Different Pretreatment Methods on the Bioconversion of Rice Bran into Ethanol", Mycobiology, 32(4), pp.170~172 (2004), 한국균학회
- Goshima T, "Bioethanol production from lignocellulosic biomass by a novel Kluyveromyces marxianus strain", Biosci, Biotechnol. Biochem, 77, pp.1505~1510 (2013)
- Guillamón JM, "Rapid identification of wine yeast species based on RFLP analysis of the ribosomal internal transcribed spacer(ITS) region", Arch. Microbiol, 169, pp.387~392 (1998)
- 정태수, "목질계 바이오매스의 묽은 산 가수분해 공정에서 포도당 분해물 거동", KSBB Journal, 24(3), pp.267~272 (2009), 한국생물공학회
- 강현우, "섬유질계 동시당화발효를 위한 내열성 융합 효모, Kluyveromyces marxianus CHY1612의 개발", KSBB Journal, 25(6), pp.565~571 (2010), 한국생물공학회
- 김학곤, "전처리된 억새로부터 효소당화 최적화를 통한 바이오에탄올 생산", 농업생명과학연구, 49(4), pp.135~145 (2015), 농업생명과학연구원[DOI]
- Kim HJ, "The condition affecting ethanol tolerance of yeast strains in alcohol fermentaion-study on the fermentation temperature and substrate type", Korean J. Biotechnol. Bioeng, 4, pp.167~171 (1989)
- 김지연, "Isolation of Protease-producing Yeast, Pichia farinosa CO-2 and Characterization of Its Extracellular Enzyme", Applied Biological Chemistry, 53(2), pp.133~141 (2010), 한국응용생명화학회
- Kim MS, "Protoplast fusion of Saccharomyces and Kluyveromyces to develop thermotolerant ethanol-producing yeast strains", Korean J. Appl. Microbiol. Biotechnol, 28, pp.80~86 (2000)
- 고재중, "목질계 바이오에탄올 제조공정에서 열화학적 전처리에 관한 고찰", 유기물자원화, 16(1), pp.79~88 (2008), 유기성자원학회
- 이종섭, "효모를 이용한 에탄올 생산을 위한 감껍질 전처리조건의 최적화", 한국미생물·생명공학회지, 42(2), pp.202~206 (2014), 한국미생물·생명공학회[DOI]
- 이경은, "작물의 성분 함량이 바이오에탄올 생산에 미치는 영향", 한국작물학회지, 53(3), pp.339~346 (2008), 한국작물학회
- Mallet S, "Insights into the life cycle of yeasts from the CTG clade revealed by the analysis of the Millerozyma(Pichia) farinosa species complex", Plos One, 7, pp.e35842~ (2012)
- Mclaren JS, "Crop biotechnology provides an opportunity to develop a sustainable future", Trends Biotechnol, 23, pp.339~342 (2005)
- Novak M, "Alcoholic fermentation: on the inhibitory effect of ethanol", Biotechnol. Bioeng, 23, pp.201~211 (1981)
- Oh KK, "Optimization of ammonia recycled percolation process for lignocellulosic biomass pretreatment", Korean J. Chem. Eng, 36, pp.784~791 (1998)
- 박아황, "Genome shuffling을 이용한 에탄올 생산 및 내성 효모 균주의 육종", 생명과학회지, 23(10), pp.1192~1198 (2013), 한국생명과학회[DOI]
- Sassner P, "Techno-economic evaluation of bioethanol production from three different lignocellulosic materials", Biomass Bioenerg, 32, pp.422~430 (2008)
- Shi DJ, "Genome shuffling to improve thermotolerance, ethanol tolerance and ethanol productivity of Saccharomyces cerevisiae", J. Microbiol. Biotechnol, 36, pp.139~147 (2009)
- 심현수, "김치에서 분리된 Lactobacillus buchneri의 젖산 생산 특성", 한국미생물·생명공학회지, 43(3), pp.286~290 (2015), 한국미생물·생명공학회[DOI]
- Wei Q, "Profiling of dynamic changes in the microbial community during the soy sauce fermentation process", Appl. Microbiol. Biotechnol, 97, pp.9111~9119 (2013)