Induced Disease Resistance of Satsuma Mandarins against Penicillium digitatum by Gamma Irradiation
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Gamma irradiation, which is a type of ionizing radiation, can be used as a fruit inducible factor. In the present study, the effects of gamma irradiation on the resistance of mandarin fruits against Penicillium digitatum, the causal agent of postharvest green mold disease, were investigated. Pretreatment of a low dose of gamma irradiation effectively reduced the disease incidence and lesion diameter of mandarin fruits inoculated with P. digatatum during storage for 14 d. Interestingly, exposed to 400 Gy of gamma irradiation significantly maintained firmness and stimulated the synthesis of defense-related enzymes, (e.g., β-1,3-glucanase, phenylalanine, peroxidase, and polyphenol oxidase) and pathogenesis-related (PR) genes (e.g., PR-1 and PR-2). Therefore, the gamma irradiation-induced resistance against P. digatatum involves both changes of phenolic compounds and the induction of expression of defense-related genes. In addition, scanning electron microscopy analysis revealed that induced disease resistance by gamma irradiation significantly inhibits the growth of P. digatatum in mandarin fruits. These results suggest that the exposure of gamma irradiation is a potential methods for inducing the disease resistance of fruit to postharvest fungal pathogens and for extending the postharvest life of mandarin fruit.
Abstract
Gamma irradiation, which is a type of ionizing radiation, can be used as a fruit inducible factor. In the present study, the effects of gamma irradiation on the resistance of mandarin fruits against Penicillium digitatum, the causal agent of postharvest green mold disease, were investigated. Pretreatment of a low dose of gamma irradiation effectively reduced the disease incidence and lesion diameter of mandarin fruits inoculated with P. digatatum during storage for 14 d. Interestingly, exposed to 400 Gy of gamma irradiation significantly maintained firmness and stimulated the synthesis of defense-related enzymes, (e.g., β-1,3-glucanase, phenylalanine, peroxidase, and polyphenol oxidase) and pathogenesis-related (PR) genes (e.g., PR-1 and PR-2). Therefore, the gamma irradiation-induced resistance against P. digatatum involves both changes of phenolic compounds and the induction of expression of defense-related genes. In addition, scanning electron microscopy analysis revealed that induced disease resistance by gamma irradiation significantly inhibits the growth of P. digatatum in mandarin fruits. These results suggest that the exposure of gamma irradiation is a potential methods for inducing the disease resistance of fruit to postharvest fungal pathogens and for extending the postharvest life of mandarin fruit.
참고문헌 (21)
- Charles MT, "Effect of maturity and storage on Rishitin accumulation disease resistance to Botrytis cinerea in UV-C treated tomato fruit", Acta Hortic, 599, pp.573~576 (2003)
- Daayf F, "Phenolic compounds in plant defense and pathogen counter-defense mechanisms", Recent Adv. Polyphenol Res, 3, pp.191~ (2012)
- Droby S, "Improving quality and safety of fresh and vegetables after harvest by the use of biocontrol agents and natural materials", Acta Hortic, 709, pp.45~51 (2006)
- Faize M, "Acibenzolar-S-Methyl-induced resistance to Japanese pear scab is associated with potentiation of multiple defense responses", Phytopathology, 94, pp.604~612 (2004)
- Feliziani E, "Preand postharvest treatment with alternatives to synthetic fungicides to control postharvest decay of sweet cherry", Postharvest Biol. Technol, 78, pp.133~138 (2013)
- Geweely NSI, "Sensitivity to gamma irradiation of post-harvest pathogens of pear", Int J Agric Biol, 8, pp.710~716 (2006)
- Hallman GJ, "Phytosanitary applications of irradiation", Compr Rev Food Sci F, 10, pp.143~151 (2011)
- Jeong RD, "Inhibitory effect of gamma irradiation and its application for control of postharvest green mold decay of Satsuma mandarins", Int J Food Microbiol, 234, pp.1~8 (2016)
- Jeong JY, "Effect of the combined treatment with gamma irradiation and sodium dichloroisocyanurate on postharvest Rhizopus soft of sweet potato", J Plant Dis Protect, 121, pp.243~249 (2014)
- Mares D, "Antifungal activity of Tagetes patula extracts on some phytopathogenic fungi: ultrastructural evidence on Pythium ultimum", Microbiol Res, 159, pp.295~304 (2004)
- Mohammadi M, "Changes in peroxidase and polyphenol oxidase activities in susceptible and resistant wheat heads inoculated with Fusarium graminearum and induced resistance", Plant Sci, 162, pp.491~498 (2002)
- Nigro F, "Use of UV-C to reduce storage rot of tablegrape", Postharvest Biol Technol, 13, pp.171~181 (1998)
- Ouhibi C, "Effect of UV-C radiation on resistance of Romaine lettuce (Lactuca sativa L.) against Botrytis cinerea and Sclerotinia minor", J Phytopathol, 163, pp.578~582 (2015)
- Patil BS, "Irradiation and storage influence on bioactive components and quality of early and late season ‘Rio Red’ grapefruit (Citrus paradise Macf.)", Postharvest Biol Technol, 34, pp.53~64 (2004)
- Piri I, "The use of gamma irradiation in agriculture", Afr J of Microbiol Res, 5, pp.5806~5011 (2011)
- Romanazzi G, "Recent advances on the use of natural and safe alternatives to conventional methods to control postharvest gray mold of table grapes", Postharvest Biol Technol, 63, pp.141~147 (2012)
- Salem E, "Extending the shelf-life of pear fruits by using gamma irradiation", Arab J Nucl Sci Appl, 47, pp.231~238 (2014)
- Shi J, "Control of Phytophthora nicotiana disease, induction of defense responses and genes expression of papaya fruits treated with Pseudomonas putida MGP1", J Sci Food Agric, 93, pp.568~574 (2013)
- Sumira J, "Enhancement in furanocoumarin content and phenylalanine ammonia lyase activity in developing seedlings of Psoralea corylifolia L. in response to gamma irradiation of seeds", Radiat Environ Biophys, 51, pp.341~347 (2012)
- Yao HJ, "Effect of pre- and post- harvest application of salicylic acid or methyl jasmonate on inducing disease resistance of sweet cherry fruit in storage", Postharvest Biol Technol, 35, pp.253~262 (2005)
- Zhu X, "Benzothiadiazole-mediated induced resistance to Colletotrochum musae and delayed ripening of harvested banana fruit", J Agric Food Chem, 64, pp.1494~1502 (2016)