GC-ECD를 이용한 농산물 중 제초제 aclonifen의 공정분석법 확립
Development of an Official Analytical Method for Determination of Aclonifen in Agricultural Products Using GC-ECD
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BACKGROUND: Aclonifen is used as a systemic andselective herbicide to control a wide spectrum broad-leafweeds by inhibition carotenoid biosynthesis, and then itsMRLs(Maximum Residue Limits) will be determined inonion and garlic. In this study, a new official method wasdeveloped for aclonifen determination in agriculturalproducts to routinely inspect the violation of MRL as wellas to evaluate the terminal residue level. METHODS AND RESULTS: Aclonifen was extractedfrom crop samples with acetone and the extract waspartitioned with dichloromethane and then purified bysilica solid phase extraction(SPE) cartridge. The purifiedsamples were detected GC using an ECD detector. Limits ofdetection(LOD) was 0.001 mg/kg and quantification(LOQ)was 0.005 mg/kg, respectively. For validation purposes,recovery studies were carried out at three differentconcentration levels (LOQ, 10×LOQ, 50×LOQ, n=5). Therecoveries were ranged from 74.3 to 95.0% with relativestandard deviations(RSDs) of less than 8%. All values wereconsistent with the criteria ranges requested in the Codexguidelines(CAC/GL 40). CONCLUSION: The proposed analytical method wasaccurate, effective and sensitive for aclonifen determinationand it will be used to as an official method in Korea.
Abstract
BACKGROUND: Aclonifen is used as a systemic andselective herbicide to control a wide spectrum broad-leafweeds by inhibition carotenoid biosynthesis, and then itsMRLs(Maximum Residue Limits) will be determined inonion and garlic. In this study, a new official method wasdeveloped for aclonifen determination in agriculturalproducts to routinely inspect the violation of MRL as wellas to evaluate the terminal residue level. METHODS AND RESULTS: Aclonifen was extractedfrom crop samples with acetone and the extract waspartitioned with dichloromethane and then purified bysilica solid phase extraction(SPE) cartridge. The purifiedsamples were detected GC using an ECD detector. Limits ofdetection(LOD) was 0.001 mg/kg and quantification(LOQ)was 0.005 mg/kg, respectively. For validation purposes,recovery studies were carried out at three differentconcentration levels (LOQ, 10×LOQ, 50×LOQ, n=5). Therecoveries were ranged from 74.3 to 95.0% with relativestandard deviations(RSDs) of less than 8%. All values wereconsistent with the criteria ranges requested in the Codexguidelines(CAC/GL 40). CONCLUSION: The proposed analytical method wasaccurate, effective and sensitive for aclonifen determinationand it will be used to as an official method in Korea.
참고문헌 (7)
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