파골세포 분화에 복령 추출물이 미치는 영향
Effect of Hoelen in RANKL-induced Osteoclast Differentiation
1
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
Osteoporosis is an important public health issue in postmenopausal women. It is a major public health concern and is widely believed that osteoporosis results from imbalance between bone resorption and bone formation. Recently natural products from plants have been extensively studied as therapeutic drugs to treat and prevent various diseases. Hoelen (scientific name, Poria cocos) is a mushroom that is used in traditional Chinese medicine. Hoelen exhibits anti-inflammatory activity and has a protective effect on tumor progression. However, the effect of hoelen in osteoclast differentiation remains unknown. Thus, we examined the effect of hoelen in receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation. Hoelen significantly inhibited RANKL-induced osteoclast differentiation in bone marrow-derived macrophages (BMMs) in dose dependent manner without toxicity. Also, we showed that hoelen significantly inhibited the mRNA expression of tartrate-resistant acid phophatase (TRAP) and nuclear factor of activated T cells 1 (NFATc1) in BMMs treated with RANKL. In Particular, hoelen greatly inhibited the protein expression of NFATc1. Ectopic expression of NFATc1 partially reverses hoelen-mediated inhibition of osteoclast differentiation. Taken together, our results demonstrated that hoelen may be useful treatment option of bone-related disease such as osteoporosis, reumatoid arthritis, and periodontitis.
Abstract
Osteoporosis is an important public health issue in postmenopausal women. It is a major public health concern and is widely believed that osteoporosis results from imbalance between bone resorption and bone formation. Recently natural products from plants have been extensively studied as therapeutic drugs to treat and prevent various diseases. Hoelen (scientific name, Poria cocos) is a mushroom that is used in traditional Chinese medicine. Hoelen exhibits anti-inflammatory activity and has a protective effect on tumor progression. However, the effect of hoelen in osteoclast differentiation remains unknown. Thus, we examined the effect of hoelen in receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation. Hoelen significantly inhibited RANKL-induced osteoclast differentiation in bone marrow-derived macrophages (BMMs) in dose dependent manner without toxicity. Also, we showed that hoelen significantly inhibited the mRNA expression of tartrate-resistant acid phophatase (TRAP) and nuclear factor of activated T cells 1 (NFATc1) in BMMs treated with RANKL. In Particular, hoelen greatly inhibited the protein expression of NFATc1. Ectopic expression of NFATc1 partially reverses hoelen-mediated inhibition of osteoclast differentiation. Taken together, our results demonstrated that hoelen may be useful treatment option of bone-related disease such as osteoporosis, reumatoid arthritis, and periodontitis.
참고문헌 (19)
- Goltzman, D, "Discoveries, drugs and skeletal disorders", Nat Rev Drug Discov, 10, pp.784~796 (2002)
- Ballabriga, A., "Morphological and physiological changes during growth: an update", Eur. J. Clin. Nutr, 54(suppl1), pp.S1~S6 (2000)
- Seeman, E, "Is a change in bone mineral density a sensitive and specific surrogate of anti-fracture efficacy", Bone, 41, pp.308~317 (2007)
- Becker, D. J., "The societal burden of osteoporosis", Curr. Rheumatol. Rep, 12, pp.186~191 (2010)
- Rodan, G. A., "Therapeutic approaches to bone diseases", Science, 289, pp.1508~1514 (2000)
- Ilich, J. Z., "Nutrition in bone health revisited: a story beyond calcium", J Am Coll Nutr, 19, pp.715~737 (2000)
- Teitelbaum, S. L, "Bone resorption by osteoclasts", Science, 289, pp.1504~1508 (2000)
- Takayanagi, H, "Mechanistic insight into osteoclast differentiation in psteoimmunology", J. Mol. Med, 83, pp.170~179 (2005)
- Rao, A., "Transcription factors of the NFAT family: regulation and function", Annu. Rev. Immunol, 15, pp.707~747 (1997)
- Takayanagi, H., "Osteoimmunology: shared mechanisms and cross talk between the immune and bone systems", Nat. Rev. Immunol, 7, pp.292~304 (2007)
- Abrahamsen, B, "Bisphosphonate adverse effects, lessons from large databases", Curr Opin Rheumatol, 22(4), pp.404~409 (2010)
- 소학관, "중약대사전", pp.2312~ (1975), 상해과학기술출판사
- Zhu, Y.P, "Chinese Materia Medica", pp.311~ (1998), Harwood Academic Publisher
- Korczowska, I., "Does low-dose and short-term glucocorticoids treatment increase the risk of osteoporosis in rheumatoid arthritis female patients", Clin Rheumatol, 27(5), pp.565~572 (2008)
- 권미선, "Antimicrobial and Antitumor Activity of Triterpenoids Fraction from Poria cocos Wolf", Journal of Food Science and Nutrition, 28(5), pp.1029~1033 (1999)
- Dougall, W. C., "RANK is essential for osteoclast and lymph node development", Genes & Development, 13, pp.2412~2424 (1999)
- H. Takayanagi, "Taniguchi Developmental Cell", Taniguchi Developmental Cell, 3, pp.889~ (2002)
- M. Matsumoto, "The Journal of Biological Chemistry", The Journal of Biological Chemistry, 279, pp.45969~ (2004)
- Galibert, L., "The involvement of multiple tumor neocrosis factor receptor (TNFR)-associated factors in the signaling mechani는 of receptor activator of NF-κB, a member of the TNFR superfamily", J Biol Chem, 273, pp.34120~34127 (1998)