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HR-MAS NMR Technique for Metabolic Profiling of Powdery Ginseng

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초록

Ginseng is used as a medicinal ingredient. The quality control of species, age, origin and manufacturing process is important. The metabolome of ginseng about quality was studied in many reports. Almost studies carried out the extract of ginseng, however, the reproducibility cannot be obtained using extracted sample. In this study, powdery ginseng samples were analyzed using high resolution-magic angle spinning nuclear magnetic resonance (HR-MAS NMR)-based metabolomics except extraction step. Sample was measured three times using 600 MHz NMR spectrometer equipped with nano probe. Reproducibility can be enhanced using this method and the metabolic profiles of ginseng were identified and quantified.

Abstract

Ginseng is used as a medicinal ingredient. The quality control of species, age, origin and manufacturing process is important. The metabolome of ginseng about quality was studied in many reports. Almost studies carried out the extract of ginseng, however, the reproducibility cannot be obtained using extracted sample. In this study, powdery ginseng samples were analyzed using high resolution-magic angle spinning nuclear magnetic resonance (HR-MAS NMR)-based metabolomics except extraction step. Sample was measured three times using 600 MHz NMR spectrometer equipped with nano probe. Reproducibility can be enhanced using this method and the metabolic profiles of ginseng were identified and quantified.

참고문헌 (10)

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  4. D. Yoon, , J. Korean Soc. Magn. Reson. Med, 20, pp.13~ (2016)
  5. Jinho Kang, "NMR-Based Metabolomics Approach for the Differentiation of Ginseng (Panax ginseng) Roots from Different Origins", Archives of Pharmacal Research, 31(3), pp.330~336 (2008), 대한약학회
  6. E. J. Lee, , J. Agric. Food. Chem, 57, pp.7513~ (2009)
  7. S. O. Yang, , J. Pharm. Biomed. Anal, 58, pp.19~ (2012)
  8. C. Corsaro, , Sci. Rep, 3, pp.2896~ (2013)
  9. O. Beckonert, , Nat. Protoc, 5, pp.1019~ (2010)
  10. M. R. Pears, , J. Biol. Chem, 280, pp.52~ (2005)

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