Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
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초록
There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections.
Abstract
There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections.
참고문헌 (11)
- O’Donoghue PJ, "Cryptosporidium and cryptosporidiosis in man and animals", Int J Parasitol, 25, pp.139~195 (1995)
- Xiao L, "Cryptosporidium taxonomy: recent advances and implications for public health", Clin Microbiol Rev, 17, pp.72~97 (2004)
- Caccio SM, "Unravelling Cryptosporidium and Giardia epidemiology", Trends Parasitol, 21, pp.430~437 (2005)
- Nichols RA, "Molecular fingerprinting of Cryptosporidium oocysts isolated during water monitoring", Appl Environ Microbiol, 72, pp.5428~5435 (2006)
- Feltus DC, "Evidence supporting zoonotic transmission of Cryptosporidium spp. in Wisconsin", J Clin Microbiol, 44, pp.4303~4308 (2006)
- Lee SU, "CP2 gene as a useful viability marker for Cryptosporidium parvum", Parasitol Res, 102, pp.381~387 (2008)
- Morgan-Ryan UM, "Cryptosporidium hominis n. sp. (Apicomplexa: Cryptosporidiidae) from Homo sapiens", J Eukaryot Microbiol, 49, pp.433~440 (2002)
- Jothikumar N, "Detection and differentiation of Cryptosporidium hominis and Cryptosporidium parvum by dual TaqMan assays", J Med Microbiol, 57, pp.1099~1105 (2008)
- 국상미, "Genotype and animal infectivity of a human isolate of Cryptosporidium parvum in the Republic of Korea (Brief Communication)", The Korean Journal of Parasitology, 42(2), pp.85~89 (2004), 대한기생충학회
- 박재환, "Genotype analysis of Cryptosporidium spp. Prevalent in a rural village in Hwasun-gun, Republic of Korea", The Korean Journal of Parasitology, 44(1), pp.27~33 (2006), 대한기생충학회
- Cheun HI, "Genotypic characterization of Cryptosporidium oocysts isolated from healthy people in three different counties of Korea", J Vet Med Sci, 69, pp.1099~1101 (2007)