과학수사 상 추적자로써 작물의 재배토양 및 영양성분의군집 분석결과; 시금치를 중심으로
Cluster Analytical Results of Nutritional Component and Crop Soil as a Tracer in Forensic Science: Focused on Spinach
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재배지역 별 시금치에 대한 아미노산, 지방산, glycine betaine 및 토양 중 원소에 대한 군집분석 결과로부터 과학수사학적인 개체식별자(marker)로써 활용 가능성을 검토하였다. 전체 10종의 시금치시료 중 16종의 아미노산과 11종의 지방산 분석결과에 근거하여 각 3종의 아미노산(asparatic acid, methionine 및 proline)과 지방산(linolenic acid (C18:3), myristoleic acid (C14:1) 및 myristic acid (C14:0)) 분자들이 군집성을 부여할 수 있는 성분이었다. 또한, 시금치 중 glycine betaine의 함량은 해안지역에서 재배된 시료에서 K+(spinach)/Na+(soil)의 조성 비율이 높았으며 재배토양의 무기이온 농도는 바닷바람으로 인한 염류스트레스(salt stress) 환경과 인과관계가 있었다. 그리고, glycine betaine, K+(sp)/Na+(s), 칼슘(Ca)과 실리콘(Si), 티타늄(Ti) 및 바나듐(V) 등의 함유량 분석결과도 군집성 식별인자가 될 수 있음을 암시하였다. 그러므로 재배환경 및 지역에 따른 식물류, 토양성분 및 무기질 함량에 따른 군집분석 결과는 과학수사 상 추적자로써 활용이 가능할 것으로 판단되었다.
Abstract
The purpose of study is to investigate the applicability of forensical maker in scientific investigations based on growth characteristics and components of amino acids, fatty acids, glycine betaine, and mineral contents of the soil properties by spinach cultivating area for various location of Korea. Based on the results of the analysis of the 16 amino acids and 11 fatty acids in all 10 strains of spinach, Cluster analysis revealed that aspartic acid, methionine, and proline, among amino acids, and linolenic acid (C18:3), myristoleic acid (C14:1), and myristic acid (C14:0), among the fatty acids, were potential components to distinguish between clusters. In addition, the content of glycine betaine was high in the samples cultivated in the coastal area, in relation to K+(spinach) / Na+(soil) forming ratio and in the growing soil. And, the content identification factors of glycine betaine, K+(sp)/Na+(s), calcium (Ca) and silicon (Si), titanium (ti) and vanadium (V) are collected. Therefore, the analysis results associated with the characteristics of the various plant's cultivation environment, areas and the mineral contents by the soil properties confirmed the possibility to utilize the forensic marker function.
참고문헌 (38)
- PA Bull, "The Forensic Analysis of Soils and Sediment Take from the Cast of a Footprint", Forensic Sci Int, 162, pp.6~12 (2006)
- R Schubbert, "Food Forensics: Analysis of Food, Raw and Processed Materials with Molecular Biological Methods", Forensic Sci Int:Genetics Supplement Series, 1, pp.616~619 (2008)
- S Prim-rose, "Food forensics:methods for determining the authenticity of foodstuffs", Trends in Food Sci & Technol, 21, pp.582~590 (2010)
- VF Melo, "Chemical, Physical and Mineralogical Characterization of Soils from the Curitiba Metropolitan Region for Forensic Purpose", Forensic Sci Int, 179, pp.123~134 (2008)
- S Akter, "Chemical and Nutritional Composition of Terminalia ferdinandiana (Kakadu Plum)", Foods, 7(60), pp.1~11 (2018)
- FAT Serafim, "Chemical data as markers of the geographical origins of sugarcane spirits", Food Chemistry, 196, pp.196~203 (2016)
- JL Horreoa, "Forensic assignment to geographic origin, a useful tool in seafood fraud control", Forensic Sci Int, 272, pp.37~40 (2017)
- AM Iqbal, "Forensic decomposition odour profiling : A review of experimental designs and analytical techniques", Trends in Anal Chem, 91, pp.112~124 (2017)
- X Zhou, "Classification the geographical origin of corn distillers dried grains with solubles by near infrared reflectance spectroscopy combined with chemometrics: A feasibility study", Food Chem, 189, pp.13~18 (2015)
- H Liu, "δ2H of wheat and soil water in different growth stages and their application potentialities as fingerprints of geographical origin", Food Chem, 226, pp.135~140 (2017)
- Z Jandric, "An investigative study on discrimination of honey of various floral and geographical origins using UPLC-QToF MS and multivariate data analysis", Food Control, 72, pp.189~197 (2017)
- K Sumberova, "Analysis of plant soil seed banks and seed dispersal vectors : Its potential and limits for forensic investigations", Forensic Sci Int, 270, pp.121~128 (2017)
- B Degena, "A nearest neighbour approach by genetic distance to the assignment of individual trees to geographic origin", Forensic Sci Int : Genetics, 27, pp.132~141 (2017)
- EK Shibuya, "Carbon and nitrogen stable isotopes as indicative of geographical origin of marijuana samples seized in the city of Sao Paulo(Brazil)", Forensic Sci Int, 167, pp.8~15 (2007)
- G Ma, "Determining the geographical origin of Chinese green tea by linear discriminant analysis of trace metals and rare earth elements : Taking Dongting Biluochun as an example", Food Control, 59, pp.714~720 (2016)
- M Necemer, "Discrimination between Slovenian cow, goat and sheep milk and cheese according to geographical origin using a combination of elemental content and stable isotope data", J Food Composition and Anal, 52, pp.16~23 (2016)
- JD Daniel, "Serpent's source: Determining the source and geographic origin of traded python skins using isotopic and elemental markers", Biological Conservation, 209, pp.406~441 (2017)
- D Rashmi, "Stable isotope ratio analysis in determining the geographical traceability of Indian wheat", Food Control, 79, pp.169~176 (2017)
- T Chen, "Variation of the light stable isotopes in the superior and inferior grains of rice (Oryza sativa L.) with different geographical origins", Food Chem, 209, pp.95~98 (2016)
- R Ogden, "Wildlife forensic science : A review of genetic geographic origin assignment", Forensic Sci Int : Genetics, 18, pp.152~159 (2015)
- B Mehari, "Profiling of phenolic compounds using UPLC-MS for determining the geographical origin of green coffee beans from Ethiopia", J Food Composition and Anal, 45, pp.16~25 (2016)
- JA Vermeulen, "Origin identification of dried distillers grains with solubles using attenuated total reflection Fourier transform mid-infrared spectroscopy after in situ oil extraction", Food Chem, 189, pp.19~26 (2015)
- S Rummel, "The Combination of Stable Isotope Abundance Ratios of H, C, N and S with 87Sr", 86Sr for Geographical Origin Assignment of Arange Juices, 2010, pp.118, 890~900
- GT Swindles, "A Preliminary Investigation into the Use of Testate Amoebae for the Discrimination of Forensic Soil Samples", Sci and Justice, 49, pp.182~190 (2009)
- M Caredda, "Building of prediction models by using Mid-Infrared spectroscopy and fatty acid profile to discriminate the geographical origin of sheep milk", LWT-Food Sci and Technol, 75, pp.131~136 (2017)
- H Liu, "Combination of the 87Sr", 86Sr ratio and light stable isotopic values (δ13C, δ15N and δD) for identifying the geographical origin of winter wheat in China, 2016, pp.212, 367~373
- E Ehler, "Forensic genetic analyses in isolated populations with examples of central European Valachs and Roma", J Forensic and Legal Med, 48, pp.46~52 (2017)
- MT Osorio, "Identification of vegetable oil botanical speciation in refined vegetable oil blends using an innovative combination of chromatographic and spectroscopic techniques", Food Chem, 189, pp.67~73 (2015)
- JE Gaiad, "Tracing the geographical origin of Argentinean lemon juices based on trace element profiles using advanced chemometric techniques", Microchem J, 129, pp.243~248 (2016)
- F Montinaro, "Using forensic microsatellites to decipher the genetic structure of linguistic and geographic isolates : A survey in the eastern Italian Alps", Forensic Sci Int : Genetics, 6, pp.827~833 (2012)
- S Mahajan, "Cold, salinity and drought stresses: An overview", Arch Biochem Biophysics, 444, pp.139~158 (2005)
- DM Catello, "Free Amino Acids and Glycine Betaine in Leaf Osmoregulation of Spinach Responding to Increasing Salt Stress", New Phytologist, 158, pp.455~463 (2003)
- R Li, "Interactive effects of various salt and alkali stresses on growth, organic solutes, and cation accumulation in a halophyte Spartina alterniflora (Poaceae)", Environ Exper Botany, 68, pp.66~74 (2010)
- M Ashraf, "Roles of Glycine Betaine and Proline in Improving Plant Abiotic Stress Resistance", Environ Experi Botany, 59, pp.206~216 (2007)
- BC chwahn, "Pharmacokinetics of oral betaine in healthy subjects and patients with homocystinuria", Br J Clin Pharmacol, 55, pp.6~13 (2003)
- A Kumar, "Does plant-Microbe interaction confer stress tolerance in plants : A review", Microbiol Res, 207, pp.41~52 (2018)
- P Moradi, "Metabolomic approach reveals the biochemical mechanisms underlying drought stress tolerance in thyme", Anal Biochem, 527, pp.49~62 (2017)
- ED Rebecca, "Comparison of Phospholipid Fatty (PLFA)and Soil Fatty Acid Methyl Ester (TSFAME) for Characterrizing Soil Microbial Comm", Soil Biol Biochem, 36, pp.1793~1800 (2004)