꼬시래기의 사분포자체와 배우체의 초기 생장에 대한 온도와 광도의 영향
Effects of Temperature and Light Intensity on the Early Growth of Tetrasporophytes and Gametophytes of Agarophyton vermiculophyllum
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The aim of this study is to examine the physiological characteristics of an agarophyte Agarophyton vermiculophyllum (Ohmi) Gurgel, J.N. Norris et Fredericq in the early life stage of tetrasporophytes (2n) and gametophytes (n) to select appropriate seedlings for mariculture. Growth experiments were carried out at the combinations of four temperatures (20, 25, 30, and 35°C) and three light intensity levels (20, 60, and 100 μmol photons m-2 s-1) in the two ontogenetic stages: discoid holdfasts and erect sporelings. Holdfast areas and sporeling lengths of tetrasporophytes and gametophytes were estimated after 14 days in culture. Relative growth rates (RGRs) for holdfast areas were 7.08–28.38% day-1 for tetrasporophytes and 11.58–23.67% day-1 for gametophytes. At 35°C, holdfasts of tetrasporophytes survived with RGRs of 7.08–23.28% day-1 but those of gametophytes died. Maximal holdfast growth of tetrasporophytes occurred at 30°C and 100 μmol photons m-2 s-1, which were different from gametophytes (25°C and 100 μmol photons m-2 s-1). RGRs of tetrasporophytic sporelings were 2.93–11.11% day-1 and were between 0.78–10.82% day-1 for gametophytes. Maximal growth of A. vermiculophyllum sporelings occurred at 25°C and 60 μmol photons m-2 s-1 for tetrasporophytes, and at 20°C and 100 μmol photons m-2 s-1 for gametophytes. In conclusion, the present results indicate that carpospores could be used as resources of spore-seedling methods having genetic diversity for mass field cultivation because tetrasporophytes showed higher-temperature tolerance and faster-growing ability than gametophytes of A. vermiculophyllum in the discoid holdfast and sporeling stages.
Abstract
The aim of this study is to examine the physiological characteristics of an agarophyte Agarophyton vermiculophyllum (Ohmi) Gurgel, J.N. Norris et Fredericq in the early life stage of tetrasporophytes (2n) and gametophytes (n) to select appropriate seedlings for mariculture. Growth experiments were carried out at the combinations of four temperatures (20, 25, 30, and 35°C) and three light intensity levels (20, 60, and 100 μmol photons m-2 s-1) in the two ontogenetic stages: discoid holdfasts and erect sporelings. Holdfast areas and sporeling lengths of tetrasporophytes and gametophytes were estimated after 14 days in culture. Relative growth rates (RGRs) for holdfast areas were 7.08–28.38% day-1 for tetrasporophytes and 11.58–23.67% day-1 for gametophytes. At 35°C, holdfasts of tetrasporophytes survived with RGRs of 7.08–23.28% day-1 but those of gametophytes died. Maximal holdfast growth of tetrasporophytes occurred at 30°C and 100 μmol photons m-2 s-1, which were different from gametophytes (25°C and 100 μmol photons m-2 s-1). RGRs of tetrasporophytic sporelings were 2.93–11.11% day-1 and were between 0.78–10.82% day-1 for gametophytes. Maximal growth of A. vermiculophyllum sporelings occurred at 25°C and 60 μmol photons m-2 s-1 for tetrasporophytes, and at 20°C and 100 μmol photons m-2 s-1 for gametophytes. In conclusion, the present results indicate that carpospores could be used as resources of spore-seedling methods having genetic diversity for mass field cultivation because tetrasporophytes showed higher-temperature tolerance and faster-growing ability than gametophytes of A. vermiculophyllum in the discoid holdfast and sporeling stages.
참고문헌 (19)
- Alveal KHCEC, "Mass cultivation of the agar-producing alga Gracilaria chilensis(Rhodophyta)from spores", Aquaculture, 148, pp.77~83 (1997)
- Buschmann AH, "Red algal farming in Chile : A review", Aquaculture, 194, pp.203~220 (2001)
- Glenn EP, "Spore culture of the edible red seaweed, Gracilaria parvispora(Rhodophyta)", Aquaculture, 142, pp.59~74 (1996)
- Guillemin ML, "Differential ecological responses to environmental stress in the life history phases of the isomorphic red alga Gracilaria chilensis(Rhodophyta)", J Appl Phycol, 25, pp.215~224 (2013)
- Guzmán-Urióstegui A, "Factors affecting sporulation of Gracilaria cornea(Gracilariales, Rhodophyta)carposporophytes from Yucatan, Mexico", Hydrobiologia, 398, pp.285~290 (1999)
- Jayasankar R, "Cultivation of marine red alga Gracilaria edulis(Gigartinales, Rhodophyta)from spores", Indian J Mar Sci, 31, pp.75~77 (2002)
- Kain JM, "A review of the life history, reproduction and phenology of Gracilaria", J Appl Phycol, 7, pp.269~281 (1995)
- Kim YS, "Reproductive phenology of Gracilaria verrucosa(Rhodophyta)in Cheongsapo near Pusan, Korea", J Fish Sci Tech, 1, pp.147~151 (1998)
- Kim YS, "Effects of light, desiccation and salinity for the spore discharge of Gracilaria verrucosa(Rhodophyta) in Korea", J Fish Sci Tech, 4, pp.257~260 (2001)
- Mantri VA, "The carpospore culture of industrially important red alga Gracilaria dura(Gracilariales, Rhodophyta)", Aquaculture, 297, pp.85~90 (2009)
- Karina M. Michetti, "Carpospore release and sporeling development in Gracilaria gracilis (Gracilariales, Rhodophyta) from the southwestern Atlantic coast (Chubut, Argentina)", Journal of Applied Phycology, 25(6), pp.1917~1924 (2013), Springer Science and Business Media LLC[DOI]
- J. Orduña-Rojas, "Effects of Irradiance and Temperature on the Release and Growth of Carpospores from Gracilaria cornea J. Agardh (Gracilariales, Rhodophyta)", Botanica Marina, 42(4) (1999), Walter de Gruyter GmbH[DOI]
- Provasoli L, "Media and prospects for the cultivation of marine algae", Proceeding of the US-Japan conference, pp.63~75 (1968), Japanese Society for Plant Physiology
- Polifrone M, "Alternative pathways in the life history of Gracilaria gracilis(Gracilariales, Rhodophyta)from north-eastern Sicily(Italy)", Aquaculture, 261, pp.1003~1013 (2006)
- Yukihiko Serisawa, "Growth of Ecklonia cava (Laminariales, Phaeophyta) sporophytes transplanted to a locality with different temperature conditions", Phycological Research, 50(3), pp.201~207 (2002), Wiley[DOI]
- Shang YC, "Economic aspects of Gracilaria culture in Taiwan", Aquaculture, 8, pp.1~7 (1976)
- Sokal RR, "Biometry", pp.880~ (1995), Freeman
- Yokoya NS, "Effects of environmental factors and plant growth regulators on growth of the red alga Gracilaria vermiculophylla from Shikoku Island, Japan", Hydrobiologia, 398, pp.339~347 (1999)
- Zemke-White WL, "World seaweed utilization : An end of century summary", J Appl Phycol, 11, pp.369~376 (1999)