식중독 유발 세균의 증식에 미치는 패장과 연교 추출물의 상승 효과
Synergistic Antimicrobial Effect of Patrinia scabiosaefolia and Forsythiae fructus Extracts on FoodbornePathogens.
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초록
본 연구에서는 식중독 유발세균에 대한 항균활성이 우수한 천연 항균성 물질을 검색하기 위해 예로부터 민간과 한방에서 널리 이용되어 온 패장을 각종 유기용매로 추출하여식중독 유발세균에 대한 항균활성을 조사해 보았다. 패장을petroleum ether, chloroform, ethly acetate, methanol을이용하여 실온에서 각각 용매별로 계통 분획하고, 열수추출물을 얻은 후, 9종의 식중독 유발세균(S. aureus, S.epidermidis, Salmonella paratyphimurium, S. flexneri, E.coli, Salmonella typhimurium, S. dysenteriae, P. aeruginosa,S. sonnei)에 대하여 항균효과를 조사하였다. 패장 추출물의농도별 항균 활성 검색에서는 패장의 ethyl acetate 추출물이가장 큰 항균 효과를 보였으며 E. coli와 S. sonnei가 가장민감하게 반응하는 균주였다. 패장의 ethyl acetate 추출물과연교의 물 추출물을 혼합하여 항균력을 측정해 본 결과 두추출물을 섞어 첨가했을 경우가 단독으로 사용했을 시 보다약 20% 정도의 더 큰 상승효과를 나타내었다. 또한 패장의ethyl acetate 추출물이 식중독 유발세균의 성장에 미치는 효과를 검정하기 위해 S. epidermidis 및 S. sonnei의 배양액에패장의 ethyl acetate 추출물을 각각 4,000 ppm 농도로 첨가했을 시, S. epidermidis의 생육이 72시간 이상까지 억제됨을 관찰할 수 있었고, S. sonnei의 생육도 72시간까지 지연시킬 수 있었다. 이상의 결과 패장의 ethyl acetate 추출물은S. epidermidis와 S. sonnei의 성장을 효과적으로 억제시킴을알 수 있었다.
Abstract
To investigate the antimicrobial effect of the Patrinia scabiosaefolia extracts against food-borne pathogens, we extracted the P. scabiosaefolia with methanol at room temperature and the fractionation of the methanol extracts was carried out by using petroleum ether, chloroform, ethyl acetate, and methanol, respectively. The antimicrobial activity of the P. scabiosaefolia extracts was determined by using a paper disc method against food-borne pathogens and food spoilage bacteria. The ethyl acetate extracts of P. scabiosaefolia showed the highest antimicrobial activity against Escherichia coli and Shigella sonnei. Synergistic effect in inhibition was observed when P. scabiosaefolia extract was mixed Forsythiae fructus extract as compared to each extracts alone. Finally, the growth inhibition curves were determined by using ethyl acetate extracts of P. scabiosaefolia against Staphylococcus epidermidis and Shigella sonnei. The ethyl acetate extract of P. scabiosaefolia had strong antimicrobial activity against S. sonnei at the concentration of 4,000 ppm. At this concentration, the growth of S. Sonnei was retarded more than 72 hours and up to 48 hours for S. epidermidis. These results sugges that the ethyl acetate extracts of P. scabiosaefolia can be used for the efficient material against the growth of S. epidermidis and S. sonnei.
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