A Study on the Single-dose Oral Toxicity of Super Key in Sprague-Dawley Rats
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Objectives: This study was performed to analyze the single-dose oral toxicity of the super key (processed sulfur). Methods: All experiments were conducted at Medvill, an institution authorized to perform non-clinical studies, under the Good Laboratory Practice (GLP) regulations. In order to investigate the oral toxicity of super key. We administered it orally to Sprague-Dawley (SD) rats. The SD rats were divided into four groups of five male and five female animals per group: group 1 being the control group and groups 2, 3, and 4 being the experimental groups. Doses of super key 500 mg/kg, 1,000 mg/kg and 2,000 mg/kg were administered to the experimental groups, and a dose of normal saline solution, 10 mL/kg, was administered to the control group. We examined the survival rates, weights, clinical signs, gross findings and necropsy findings. This study was conducted under the approval of the Institutional Animal Ethics Committee. (Approval number: A01-14018). Results: No deaths or abnormalities occurred in any of the four groups. Although slight decreases in the weights of some female rats were noted, no significant changes in weights or differences in the gross findings between the control group and the experimental groups were observed. To check for abnormalities in organs, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs. Conclusion: The results of this research showed that administration of 500 ─ 2,000 mg/kg of super key did not cause any changes in the weights or in the results of necropsy examinations. Neither did it result in any mortalities. The above findings suggest that treatment with super key is relatively safe. Further studies on this subject are needed to yield more concrete evidence.
Abstract
Objectives: This study was performed to analyze the single-dose oral toxicity of the super key (processed sulfur). Methods: All experiments were conducted at Medvill, an institution authorized to perform non-clinical studies, under the Good Laboratory Practice (GLP) regulations. In order to investigate the oral toxicity of super key. We administered it orally to Sprague-Dawley (SD) rats. The SD rats were divided into four groups of five male and five female animals per group: group 1 being the control group and groups 2, 3, and 4 being the experimental groups. Doses of super key 500 mg/kg, 1,000 mg/kg and 2,000 mg/kg were administered to the experimental groups, and a dose of normal saline solution, 10 mL/kg, was administered to the control group. We examined the survival rates, weights, clinical signs, gross findings and necropsy findings. This study was conducted under the approval of the Institutional Animal Ethics Committee. (Approval number: A01-14018). Results: No deaths or abnormalities occurred in any of the four groups. Although slight decreases in the weights of some female rats were noted, no significant changes in weights or differences in the gross findings between the control group and the experimental groups were observed. To check for abnormalities in organs, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs. Conclusion: The results of this research showed that administration of 500 ─ 2,000 mg/kg of super key did not cause any changes in the weights or in the results of necropsy examinations. Neither did it result in any mortalities. The above findings suggest that treatment with super key is relatively safe. Further studies on this subject are needed to yield more concrete evidence.
참고문헌 (17)
- 신흥묵, "HMC05의 Sprague-Dawley 휜쥐를 이용한 4주 반복 경구투여 DRF 독성시험", 대한한의학회지, 30(5), pp.102~114 (2009), 대한한의학회
- Lee MY, "Genotoxicity evaluation of guibi-tang extract using an in vitro bacterial reverse mutation assay, chromosome aberration assay, and in vivo micronucleus test", BMC Complement Altern Med, 14(1), pp.215~222 (2014)
- Yuan JB, "Acute and subacute toxicity of the extract of Aristolochiae fructus and honey-fried Aristolochiae fructus in rodents", Biol Pharm Bull, 37(3), pp.387~393 (2014)
- Ingenbleek Y, "The nutritional relationship linking sulfur to nitrogen in living organisms", J Nutr, 136(6), pp.1641~1651 (2006)
- Heo J, "Dongeui science institute, treasured mirror of eastern medicine (東醫寶鑑)", pp.2035~ (2007), Bubin publishers
- Pruksachatkunakorn C, "Sulfur for scabies outbreaks in orphanages", Pediatr Dermatol, 19(5), pp.448~453 (2002)
- Kenawi MZ, "Treatment of human scabies by sulfur and permethrin", J Egypt Soc Parasitol, 23(3), pp.691~696 (1993)
- Gupta AK, "The use of sulfur in dermatology", J Drugs Dermatol, 3(4), pp.427~431 (2003)
- 박건영, "유황처리 열무로 제조한 열무김치의 특성과 인체 위암세포의 성장억제효과", 한국식품영양과학회지, 35(2), pp.158~163 (2006), 한국식품영양과학회
- Kun-Young Park, "Effect of Sulfur Enriched Young Radish Kimchi on the Induction of Apoptosis in HT-29 Human Colon Cancer Cells", Preventive Nutrition and Food Science, 11(3), pp.184~190 (2006), 한국식품영양과학회
- 최귀헌, "유황오리 추출물의 각종 암세포에 대한 생육억제 효과", 한국축산식품학회지, 22(4), pp.348~351 (2002), 한국축산식품학회
- 하애화, "Inorganic sulfur reduces cell proliferation by inhibiting of ErbB2 and ErbB3 protein and mRNA expression in MDA-MB-231 human breast cancer cells", Nutrition Research and Practice, 7(2), pp.89~95 (2013), 한국영양학회[DOI]
- Duan F, "Sulfur inhibits the growth of androgen-independent prostate cancer in vivo", Oncol Lett, 9(1), pp.437~441 (2015)
- Parcell S, "Sulfur in human nutrition and applications in medicine", Altern Med Rev, 7(1), pp.22~44 (2002)
- Kim YK, "The principle and test method. toxicology", pp.15~18 (1994), Donghwagisul
- Kim YG, "Toxicology", pp.15~18 (1984), Donghwagisul
- "Korea Food & Drug Administration notification" (2005)[URL]