본문 바로가기
KERD
논문KCI 등재

Application of the apoptogenic pretreatment to enhance the anti-tumor immunity of the Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF)-secreting CT26 tumor cells

김영호|전도연|Elizabeth M Jaffee
대한면역학회 (The Korean Association of Immunologists)|2005.06|Immune Network|제5권 제2호|pp. 110-116

0

조회

0

다운로드

0

인용

0

참고문헌

초록

Abstract

Background: As an attempt to develop a strategy to improve the protective immune response to GM-CSF-secreting CT26 (GM-CSF/CT26) tumor vaccine, we have investigated whether the apoptogenic treatment of GM-CSF/CT26 prior to vaccination enhances the induction of anti-tumor immune response in mouse model. Methods: A carcinogen- induced mouse colorectal tumor, CT26 was transfected with GM-CSF gene using a retroviral vector to generate GM-CSF-secreting CT26 (CT26/GM-CSF). The CT26/GM-CSF was treated with γ-irradiation or mitomycin C to induce apoptosis and vaccinated into BALB/c mice. After 7 days, the mice were injected with a lethal dose of challenge live CT26 cells to examine the protective effect of tumor vaccination in vivo. Results: Although both apoptotic and necrotic CT26/GM-CSF vaccines were able to enhance anti-tumor immune response, apoptotic CT26/GM-CSF induced by pretreatment with γ-irradiation (50,000 rads) was the most potent in generating the anti-tumor immunity, and thus 100% of mice vaccinated with the apoptotic cells remained tumor free for more than 60 days after tumor challenge. Conclusion: Apoptogenic pretreatment of GM-CSF-secreting CT26 tumor vaccine by γ-irradiation (50,000 rads) resulted in a significant enhancement in inducing the protective anti-tumor immunity. A rapid induction of apoptosis of CT26/GM-CSF tumor vaccine at the vaccine site might be critical for the enhancement in anti-tumor immune response to tumor vaccine.

참고문헌 (26)

  1. "Interleukin-2 gene transfer into tumor cells abrogates tumorigenicity and induces protective immunity", 172, pp.1217~1224 (1990)
  2. "Vaccination with irradiated tumor cells engineered to secrete murine granulocyte-macrophage colony-stimulating factor stimulates potent", dra(ff g), pp.3539~3543 (1993)
  3. "Bioactivity of autologous irradiated renal cell carcinoma vaccines generated by ex vivo granulocyte-macrophage colony- stimulating factor gene transfer", 57, pp.1537~1546 (1997)
  4. "Vaccination with irradiated autologous melanoma cells engineered to secrete human granulocyte-macrophage colony- stimulating factor generates potent antitumor immunity in patient with meastatic melanoma", 95, pp.13141~13146 (1998)
  5. "Induction of immunity to prostate cancer antigens results of a clinical trial of vaccination with irradiated autologous prostate tumor cells engineered to secrete granulocyte-macrophage colony-stimulating factor using ex vivo gene transfer", 59, pp.5160~5168 (1999)
  6. "Novel allogenic granulocyte- macrophage colony-stimulating factor-secreting tumor vaccine for pancreatic cancer a phase 1 trial of safety and immune activation", 19, pp.145~156 (2001)
  7. "A phase I vaccine safety and chemotherapy dose-finding trial of an allogeneic GM-CSF-secreting breast cancer vaccine given in a specifically timed sequence with immunomodulatory doses of cyclophosphamide and doxorubicin", pp.313~337 (2004)
  8. "Antibodies to CD3", T-cell receptor complex induce death by apoptosis in immature T cells in thymic cultures, 1989, pp.337, 181~184
  9. "Cell death induction by receptors of the TNF family:towards a molecular understanding", 410, pp.96~106 (1997)
  10. "Autocrine T-cell suicide mediated by APO-1", (Fas, pp.1995, 373[DOI]
  11. "Oxidative stress as a mediator of apoptosis", 15, pp.7~10 (1994)
  12. "Apoptosis induced by withdrawal of trophic factors is mediated by p38 mitogen- activated protein kinase", 272, pp.20490~20494 (1997)
  13. "Heat shock induces apoptosis in mouse thymocytes and protects them from glucocorticoid-induced cell death", 143, pp.348~356 (1992)
  14. "UV radiation induces DNA fragmentation and cell death in B16 melanoma sensitized by bromodeoxyuridine:impaired c-jun induction and defective tyrosine phosphorylation signalling", 203, pp.1629~1637 (1994)
  15. "Apoptosis and dilemma of cancer chemotherapy", 89, pp.1845~1853 (1997)
  16. "The induction of tolerance by dendritic cells that have captured apoptotic cells", 191, pp.411~416 (2000)
  17. "Bystander apoptosis triggers dentritic cell maturation and antigen-presenting function", 161, pp.4467~4471 (1998)
  18. "tumor cells vaccines induce a potent immune response in vivo", 103, pp.205~211 (2003)
  19. "Enhanced expression of Fas ligand is associated with aburatubolactam C-induced apoptosis in human Jurkat T cells", 246, pp.276~281 (1998)
  20. "Cancer vaccine", 17, pp.1047~1060 (1999)
  21. "Consequences of cell death Exposure to necrotic tumor cells but not primary tissue cells or apoptotic cells induces the maturation of immunostimulatory dendritic cells", 191, pp.423~434 (2000)
  22. "Tumor antigens recognized by T cells", 18, pp.267~268 (1997)
  23. "identification of human tumor antigen by SEREX", 6, pp.208~217 (2000)
  24. "Antigen-presenting cells that phagocytose apoptotic tumor-derived cells are potent tumor vaccines", 59, pp.3329~3332 (1999)
  25. "Therapy of established tumor with a hybrid cellular vaccine generated by using granulocyte-macrophage colony- stimulating factor genetically modified dendritic cells", 97, pp.616~625 (1999)
  26. "Induction and chemotherapeutic response of two transplantable ductal adenocarcinomas of the pancreas in C57BL", 6 mice, 1984, pp.35, 2434~2439

관련 논문AI추천

Combined Treatment of an Intratumoral Injection of Dendritic Cells and Systemic Chemotherapy (Paclitaxel) for Murine Fibrosarcoma길혜윤, 김순기, 김우철, 김철수, 성도환, 송순욱, 이문희, 이민걸, 이종록, 이홍식, 임문환, 최광성연세대학교의과대학 · Yonsei Medical Journal2005 · 제46권 제6호Secretion of Biologically Active Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor by Transduced Gastric Cancer Cells박선, 김경민, 김형일, 김희영, 박길순, 신호준연세대학교의과대학 · Yonsei Medical Journal2008 · 제49권 제2호Tumor Cell Clone Expressing the Membrane-bound Form of IL-12p35 Subunit Stimulates Antitumor Immune Responses Dominated by CD8＋ T Cells김영상, 도선아, 박상민, 임호영대한면역학회 · Immune Network2013 · 제13권 제2호Irradiation of Donor Mononuclear Cells for Treatment of Chemorefractory Metastatic Solid Cancers: A Community-Based Immune Transplant Pilot StudyJohn M. Watkins, John T. Reynolds, Shrikant S. Kubsad, Tarek A. Dufan대한암학회 · Cancer Research and Treatment2012 · 제44권 제2호Cellular Mechanism of Newly Synthesized Indoledione Derivative-induced Immunological Death of Tumor Cell류정규, 백소영, 오수진, 이현아대한면역학회 · Immune Network2011 · 제11권 제6호Membrane-bound p35 Subunit of IL-12 on Tumor Cells is Functionally Equivalent to Membrane-bound Heterodimeric Single Chain IL-12 for Induction of Anti-tumor Immunity김영상, 김현진, 박상민, 이혜영대한면역학회 · Immune Network2016 · 제16권 제5호

이 간행물의 다른 논문 (8건)

암백신인상환, 손은화, 표석능pp. 55-67Opposite Roles of B7.1 and CD28 Costimulatory Molecules for Protective Immunity against HSV-2 Challenge in a gD DNA Vaccine ModelDavid B. Weiner, 신정임pp. 68-77콜라겐 유도 관절염에서 콜라겐 항원 특이 V？3+CD4+ T 세포의 선택적 증식민준기, 조미라, 민소연, 김완욱, 이정은, 박성환, 김호연, 윤종현, 이재선pp. 78-88Epitope발현 DNA vaccine과 recombinant vaccinia virus를 이용한 heterologous prime-boost vaccination에 의하여 유도되는 CD8+ T 세포 매개성 면역채준석, Abi George Aleyas, 박성옥, 어성국, 이존화, 윤현아pp. 89-98A combination of PG490 and lipopolysaccharide induce apoptosis through activation of casapase-3 and down-regulation of cIAP1 and XIAP in human astroglioma cell우경진, 권택규, 이태진, 박종욱pp. 99-104
원문 보기