본문 바로가기
KERD
논문KCI 등재

蟬蛻의 抗炎效果에 대한 實驗的 硏究

The anti-inflammatory effects of Cicadidae Periostracum

최창민|서윤정|김송백|조한백|김경완
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2011.02|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제24권 제1호|pp. 15-26

2

조회

0

다운로드

4

인용

0

참고문헌

키워드

CytokineInflammationCrytotympana pustulata(CP)lipopolysaccharide(LPS)
주제분류한의학

초록

Objectives: The purpose of this study was to investigate the anti-inflammatory effects of aqueous extract from Cicadidae Periostracum(CP) on the RAW 264.7 cells. Method: We examined the cytokine productions including nitric oxide(NO), interleukin(IL)-1b, IL-6 and tumor necrosis factor-a(TNF-a) in lipopolysaccharide (LPS)-induced RAW 264.7 cells and also inhibitory mechanisms such as mitogen -activated protein kinases(MAPKs) and nuclear factor kappa BNF-kB) using Western blot. Results: CP inhibited LPS-induced production of NO, IL-1b and TNF-a but not of IL-6 in RAW 264.7 cells. CP respectively inhibited the activation of MAPKs such as extracelluar signal-regulated kinase(ERK 1/2), c-Jun NH2-terminal kinase(JNK), p38 and NF-kB in the LPS-stimulated RAW 264.7 cells. Also oral administration of CP inhibited CLP - induced endotoxin shock. Conclusion: our results showed that CP down-regulated LPS-induced NO, IL-1b and TNF-a productions mainly through ERK, JNK, p38 MAPK and NF-kB pathway, which suggest the anti-inflammatory effects of CP.

Abstract

Objectives: The purpose of this study was to investigate the anti-inflammatory effects of aqueous extract from Cicadidae Periostracum(CP) on the RAW 264.7 cells. Method: We examined the cytokine productions including nitric oxide(NO), interleukin(IL)-1b, IL-6 and tumor necrosis factor-a(TNF-a) in lipopolysaccharide (LPS)-induced RAW 264.7 cells and also inhibitory mechanisms such as mitogen -activated protein kinases(MAPKs) and nuclear factor kappa BNF-kB) using Western blot. Results: CP inhibited LPS-induced production of NO, IL-1b and TNF-a but not of IL-6 in RAW 264.7 cells. CP respectively inhibited the activation of MAPKs such as extracelluar signal-regulated kinase(ERK 1/2), c-Jun NH2-terminal kinase(JNK), p38 and NF-kB in the LPS-stimulated RAW 264.7 cells. Also oral administration of CP inhibited CLP - induced endotoxin shock. Conclusion: our results showed that CP down-regulated LPS-induced NO, IL-1b and TNF-a productions mainly through ERK, JNK, p38 MAPK and NF-kB pathway, which suggest the anti-inflammatory effects of CP.

참고문헌 (33)

  1. 대한병리학회, "병리학", pp.65~66 (1998), 고문사
  2. Guha M, "LPS induction of gene expression in human monocytes", Cellular Signal, 13, pp.85~94 (2001)
  3. Kubes P, "Nitric oxide and intestinal inflammation", American J Medicine, 109, pp.150~158 (2000)
  4. Lundberg IE, "The role of cytokines, chemokines and adhesion molecules in the pathogenesis of idiopathic inflammatory myopathies", Current Rheumatology Report, 2, pp.216~224 (2000)
  5. 辛民敎, "臨床本草學", pp.351~352 (1997), 永林社
  6. Joong-Keun Lee, "Analgesic and Anti-Inflammatory Effect of Scutellaria baicalensis", 대한한의학회지, 28(4), pp.124~135 (2007), 대한한의학회
  7. 이태희, "LPS에 의해 유발된 炎症 스트레스에 대한 黃連과 附子의 효과", 대한본초학회지, 21(2), pp.77~85 (2006), 대한본초학회
  8. 이동언, "金銀花 및 金銀花全草가 Raw 264.7 cell에서 LPS로 유도된 NO의 생성, iNOS, COX-2 및 cytokine에 미치는 영향", 동의생리병리학회지, 19(2), pp.481~489 (2005), 대한동의병리학회
  9. 하현희, "LPS로 자극된 대식세포에서 포공영의 NO 생성 억제 효과", 한방안이비인후피부과학회지, 20(3), pp.98~106 (2007), 대한한방안이비인후피부과학회
  10. 박병모, "蟬蛻가 알레르기의 豫防에 미치는 영향", 동의병리학회지, 8(1), pp.225~234 (1993)
  11. 한범수, "곤충 생약으로부터 항응고 및 항혈전 물질의 탐색", 생약학회지, 30(4), pp.409~412 (1999)
  12. 許浚, "東醫寶鑑", 법인문화사, 489, pp.1420~1640 (1999)
  13. 송병기, "漢方婦人科學", pp.315~573 (1984), 행림출판사
  14. Peter P, "The immune system", pp.1~273 (2006), Lifescience Publishing Co
  15. Janeway CA, "Immunology. 6th ed", pp.37~489 (2005), Lifescience Publishing Co
  16. 임병우, "기초면역학", pp.201~211 (2006), 효일
  17. Horwood, "Bruton's tyrosine kinase is required for TLR2 and TLR4-induced TNF, but not IL-6 production", J Immunol, 176(6), pp.3635~3641 (2006)
  18. Aderem A, "Toll-like receptors in the induction of the innate immune response", Nature, 406(6797), pp.782~787 (2000)
  19. Nathan C, "Regulation of biosynthesis of nitric oxide", J Biol Chem, 269, pp.13725~13728 (1994)
  20. Ajizian SJ, "Specific inhibitors of p38 and extracellular signal regulated kinase mitogen-activated protein kinase pathways block inducible nitric oxide synthase and tumor necrosis factor accumulation in murine macrophages stimulated with lipopolysaccharide and interferon- gamma", J Infect Dis, 179, pp.939~944 (1999)
  21. Bryan NS, "Nitrite in nitric oxide biology: cause or consequence? A systems based review", Free Radic Biol Med, 41, pp.691~701 (2006)
  22. Janeway, Charles A, "Immunology. 6th ed", pp.37~489 (2005), Lifescience Publishing Co
  23. 김광혁, "세포·분자 면역학", pp.297~330 (1998), 정문각
  24. Tracey KJ, "Tumor necrosis factor : a pleiotropic cytokine and therapetic target", Annual Reviews of Medicine, 45, pp.491~503 (1994)
  25. Tracey KJ, "Shock and tissue injury induced by recombinant human cachectin", Science, 232, pp.977~980 (1986)
  26. dddZhao Q, "The role of mitogen activated protein kinase phosphatase-1 in the response of alveolar macrophages to lipopolysaccharide: attenuation of proinflammatory cytokine biosynthesis via feedback control of p38", J Biol Chem, 280, pp.8101~8108 (2005)
  27. Ajizian SJ, "Specific inhibitors of p38 and extracellular signal regulated kinase mitogen-activated protein kinase pathways block inducible nitric oxide synthase and tumor necrosis factor accumulation in murine macrophages stimulated with lipopolysaccharide and interferon -gamma", J Infect Dis, 179, pp.939~944 (1999)
  28. Dong Z, "Activation of tumoricidal properties in macrophages by lipopolysaccharide requires protein- tyrosine kinase activity", J Leukoc Biol, 53, pp.53~60 (1993)
  29. Cobb MH, "Dimerization in MAP-kinase signaling", Trends Biochem Sci, 25, pp.7~9 (2000)
  30. Lawrence T, "Possible new role for NF-kappaB in the resolution of inflammation", Nat Med, 7(12), pp.1291~1297 (2001)
  31. Riehemann K, "Plant extracts from stinging nettle(Urtica dioica), an antirheumatic remedy, inhibit the proinflammatory transcription factor NF-kappaB", FEBS Lett, 442(1), pp.89~94 (1999)
  32. Baeuerle PA, "Function and activation of NF-kappa B in the immune system", Annu Rev Immunol, 12, pp.141~179 (1994)
  33. Zandi E, "The IkappaB kinase complex(IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation", Cell, 91(2), pp.243~252 (1997)

관련 논문AI추천

苦木의 염증 반응 억제 효과김송백, 류익한, 서윤정, 조한백, 최창민대한한방부인과학회 · 대한한방부인과학회지2011 · 제24권 제1호槐花 물 추출물의 항염증 효과구본순, 권강범, 김민선, 김성규, 김재효, 박경철, 박성주, 배기상, 서상완, 송호준, 윤명자, 윤승원, 전호성, 정원석, 조범연, 함경완한의병리학회 · 동의생리병리학회지2009 · 제23권 제6호LPS로 유도된 염증모델에 대한 청포축어탕의 억제 효과김송백, 이수형, 정현태, 홍가경대한한방부인과학회 · 대한한방부인과학회지2021 · 제34권 제4호敗醬 물 추출물의 LPS로 유도된 RAW 264.7 세포와 mouse 염증모델에서 cytokine 및 NF-κB의 활성에 미치는 효과류익한, 송미화, 조해중, 최창민대한한방부인과학회 · 대한한방부인과학회지2017 · 제30권 제2호단삼 물 추출물의 LPS로 유도된 RAW 264.7 세포와 생쥐 염증모델에서의 항염증 효과김건희, 김송백, 조한백, 최창민, 홍가경대한한방부인과학회 · 대한한방부인과학회지2019 · 제32권 제2호Raw 264.7 세포에서 化毒丹의 항염증 효능에 대한 연구신기석, 지선영, 황보민대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2022 · 제35권 제3호

이 간행물의 다른 논문 (10건)

苦木의 염증 반응 억제 효과최창민, 서윤정, 김송백, 조한백, 류익한pp. 1-14五積散이 Estradiol Valerate로 유발된 다낭성 난소 모델에서 난포의 성숙 및 NGF발현에 미치는 영향김형준, 김종대, 서일복, 이동녕pp. 27-41갱년기 여성의 少府穴(HT8) 자침이 fMRI상의 뇌 활성 변화에 미치는 영향전규일, 김동일pp. 42-62東醫寶鑑에서 葱白의 부인과적 활용에 대한 고찰서윤정pp. 63-73배뇨장애를 호소하는 여성의 자율신경계 특성과 요검사와의 상관관계 분석이창훈, 장준복, 이진무, 박경선, 이경섭, 조정훈pp. 74-86
원문 보기