NaCl 처리가 수리취의 생장 및 생리적 특성에 미치는 영향
Effects of NaCl on Growth and Physiological Characteristics of Synurus deltoides(Aiton) Nakai
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본 연구는 NaCl 농도가 수리취의 무기이온 함량, 효소활성, 광합성 반응 및 생장특성에 미치는 영향을알아보기 위하여 수행되었으며, NaCl 농도는 4가지 처리로 조절하였다(0, 50mM, 100mM, 200mM). NaCl 처리농도가 증가할수록 최대광합성속도, 순양자수율, 탄소고정효율과 같은 광합성 활성이 감소하였으나 수분이용효율은 증가하였다. 또한 엽면적, 엽건중, 엽면적비, 줄기길이 및 T/R율이 감소하였는데 특히 100mM NaCl 농도조건에서 크게 감소하였다. 염분스트레스 처리시 이온유출량, H2O2 함량 및지질과산화(MDA)가 증가하여 세포막의 피해가 커졌으나 염분스트레스에 대응하기 위해 삼투조절물질인proline 함량도 유의적으로 증가하였고, 산화적 스트레스를 소거하기 위해 APX와 GR의 활성 역시 증가하였다. NaCl 농도가 증가할수록 엽과 뿌리의 Na 함량도 증가하였으나, Ca, K 및 Mg 함량은 이와반대되는 경향을 보였다. 결과적으로 수리취는 비교적 염분 스트레스에 민감한 식물로 특히 NaCl100mM에서 산화피해가 유의적으로 증가하는 것을 알 수 있었다.
Abstract
This study was carried out to investigate the the effects of NaCl concentration on theinorganic ion contents, antioxidative enzymes activity, photosynthetic responses and growthcharacteristics in Synurus deltoides. The treatments were regulated with the four different NaClconcentration(0 mM, 50 mM, 100 mM, 200 mM). The photosynthetic activity such as maximumphotosynthesis rate, net apparent quantum yield, carboxylation efficiency were clearly decreasedwith increasing NaCl concentration. In contrast, the water use efficiency was increased withincreasing NaCl concentration. Furthermore, the growth performances such as leaf area, leaf dryweight, specific leaf area(SLA), stem length, T/R rate were decreased with increasing NaClconcentration and especially, they significantly reduced above 100 mM NaCl concentration. Thedamage of cell membrane as a result of increased leakage of electrolytes, hydrogen peroxide,lipid peroxidation(MDA) was increased under NaCl stress. However, to protection againstdamage by NaCl stress, the proline content was significantly increased for osmotic adjustment,and also activities of the antioxidative enzymes(ascorbate peroxidase; APX), glutathione reductase; GR) for ROS(reactive oxygen species) scavenging. The Na content in both root andleaf increased with increasing NaCl concentration. However, Ca, K, and Mg content were shownthe opposite trend. These result indicate that S. deltoides was relatively higher sensitivity tooxidative stress in salinity, and oxidative damage exhibited significant increase in 100 mM ofNaCl concentration.
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