친환경 오징어(Todarodes pacificus) 간유 및 포도씨유가 고콜레스테롤을 섭취시킨 흰쥐의 지질대사에 미치는 영향
Effects of Eco-friendly Squid (Todarodes pacificus) Liver and Grape Seed Oils on the Lipid Metabolism of Rats Fed High Cholesterol
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본 연구는 친환경적으로 추출된 오징어(Todarodes pacificus) 간유와 시판 포도씨유가 고콜레스테롤을 섭취시킨 Wistar계 흰쥐의 혈청 지질 개선작용에 미치는 영향을 평가하고자 수행되었다. 실험군은식이지질의 종류에 따라 4 군으로 나누었다. 기본(BA) 식이군의 지질급원은 대두유(SO) 7%, 대조(CO)식이군은 SO 7% + 콜레스테롤 1%, 친환경 오징어 간유(EFSO) 식이군은 EFSO 7% + 콜레스테롤 1%,그리고 포도씨유(GO) 식이군은 GO 7% + 콜레스테롤 1%로 각각 조제하였다. 이들 지질을 4주 동안 섭취 시킨 흰쥐의 혈청 지질을 분석한 결과, triglyceride (TG), total-cholesterol (Total-C), LDL-C,VLDL-C 등의 함량이 CO 식이군에 비하여 EFSO 식이군에서 유의하게 낮았다 (P<0.05). 한편 GO 식이군에서 혈청 TG, VLDL-C, HDL-C 등의 함량은 EFSO 식이군의 경우와 유사하였으나 Total-C 및LDL-C의 함량은 CO 식이군과 유의적인 차이가 없었다. 또한 EFSO 식이군의 동맥경화지수(AI)는 CO식이군의 약 1/2 수준이었으며, GO 식이군에서도 이들 지수가 EFSO 식이군의 경우와 유사하였으나 외형상 약간 높은 값을 나타내었다. 그리고 모든 식이군에서 백색 및 갈색 지방조직 함량에는 유의한 차이가 없었다. 이상의 결과에서 n-3 고도불포화지방산이 풍부한 EFSO가 실험쥐의 혈청 지질, 특히LDL-C, TG 등의 함량을 저하시키는데 가장 효과적인 지질급원으로 생각된다.
Abstract
The present study aimed to evaluate the effects of the squid (Todarodes pacificus) liver andgrape seed oils on the serum lipids of rats (male Wistar, 4 weeks age) fed high cholesterol. Diet of the basal (BA) diet group was prepared according to the recommendation of theAmerican Institute of Nutrition (AIN-93G), using 7% soybean oil as lipid source. Experimentalgroups were divided into three groups according to dietary lipid sources; the control (CO)group (soybean oil 7% + cholesterol 1%), eco-friendly squid liver oil (EFSO) group (EFSO7% + cholesterol 1%) and grape seed oil (GO) group (GO 7% + cholesterol 1%). Afterfeeding for 4 weeks, the serum levels of triglyceride (TG), total cholesterol (Total-C),LDL-cholesterol (LDL-C) and VLDL-cholesterol (VLDL-C) in the EFSO group were significantly lower than those of the CO group (P<0.05). On the other hand, Total-C and LDL-C levels showed no significant differences between the GO and CO groups, while TG, VLDL-C and HDL-C levels were similar to those of the EFSO group. Atherogenic index ofthe EFSO and GO groups were lowered by a half levels of CO group. By the way, whiteand brown adipose tissue contents had no significantly difference among all the dietarygroups. These results suggest that EFSO rich in n-3 polyunsaturated fatty acids was veryeffective dietary lipid source on the lowering LDL-C or TG in serum of rats.
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