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Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

김지수|김선욱|장규태|이정웅|추영국|고기성|고기남|Kyung-A Hwang|임맑음|유재성|Ju-Taek Lee|Hyun-Ki Min|Yoon-Ju Na|Su-Bin Lee|Gislain Moussavou|Eun-Jeong Jeong
생화학분자생물학회 (Korean Society Of Biochemistry And Molecular Biology)|2012.12|BMB Reports|제45권 제12호|pp. 713-718

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ProliferationMAP kinaseCell cycleGanglioside GM1Mouse induced pluripotent stem cells (miPSCs)
주제분류화학

초록

Gangliosides play important roles in the control of several biological processes, including proliferation and transmembrane signaling. In this study, we demonstrate the effect of ganglioside GM1 on the proliferation of mouse induced pluripotent stem cells (miPSCs). The proliferation rate of miPSCs was lower than in mouse embryonic stem cells (mESCs). Fluorescence activated cell sorting analysis showed that the percentage of cells in the G2/M phase in miPSCs was lower than that in mESCs. GM1 was expressed in mESCs, but not miPSCs. To confirm the role of GM1 in miPSC proliferation,miPSCs were treated with GM1. GM1-treated miPSCs exhibited increased cell proliferation and a larger number of cells in the G2/M phase. Furthermore, phosphorylation of mitogen-activated protein kinases was increased in GM1-treated miPSCs. [BMB Reports 2012; 45(12): 713-718]

Abstract

Gangliosides play important roles in the control of several biological processes, including proliferation and transmembrane signaling. In this study, we demonstrate the effect of ganglioside GM1 on the proliferation of mouse induced pluripotent stem cells (miPSCs). The proliferation rate of miPSCs was lower than in mouse embryonic stem cells (mESCs). Fluorescence activated cell sorting analysis showed that the percentage of cells in the G2/M phase in miPSCs was lower than that in mESCs. GM1 was expressed in mESCs, but not miPSCs. To confirm the role of GM1 in miPSC proliferation,miPSCs were treated with GM1. GM1-treated miPSCs exhibited increased cell proliferation and a larger number of cells in the G2/M phase. Furthermore, phosphorylation of mitogen-activated protein kinases was increased in GM1-treated miPSCs. [BMB Reports 2012; 45(12): 713-718]

참고문헌 (39)

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