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Analysis of Enzymes of Stenotrophomonas maltophilia LK-24Associated with Phenol Degradation

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초록

Stenotrophomonas maltophilia LK-24의 페놀분해 관련 효소김정동1·강국희*1한양대학교 생명과학과, 성균관대학교 식품·생명공학과Stenotrophomonas maltophilia LK-24가 페놀화합물을 분해하는데 관련된 효소들과 페놀의 분해 산물을 분석한 결과 phenol hydrolase, catechol-2.3-dioxygenase, 2-hydroxymuconic semialdehyde dehydrogenase, 2-hydroxymuconic semialdehyde hydroxylase, 및 acetaldehyde dehydrogenase를 확인하였다. 이러한 효소들의 활성으로 보아 페놀은 meta-pathway ring cleavage를 거치면서 분해되는 것으로 사료된다. 이러한 결과는 페놀화합물의 metabolic pathways를 이해하는데 많은 도움이 되며, phenolic-contaminated waste streams에 S. maltophilia LK-24를 사용할 수 있으리라 여겨진다.

Abstract

The analysis of enzymes associated with metabolism of phenolics by Stenotrophomonas maltophilia LK-24 was conducted. To identify metabolites of phenol and phenolic compound, we investigated enzymes of S. maltophilia LK-24 associated with degradation of phenolics. We found that phenol hydrolase, catechol-2.3-dioxygenase, 2-hydroxymuconic semialdehyde dehydrogenase, 2-hydroxymuconic semialdehyde hydroxylase and acetaldehyde dehydrogenase were activated. The results showed that phenolics were gone through the meta-pathway ring cleavage. The results will contribute greatly to understand metabolic pathways of phenol and it is possible to make some assessment of the feasibility of using S. maltophilia LK-24 for the treatments of phenolic-contaminated waste streams.

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