Baicalin, baicalein and wogonin inhibits high glucose-induced vascular inflammation in vitro and in vivo
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Vascular inflammatory process has been suggested to play a key role in initiation and progression of atherosclerosis, a major complication of diabetes mellitus. Thus, in this study, we attempted to determine whether three structurally related polyphenols found in the Chinese herb Huang Qui, namely baicalin, baicalein, and wogonin, can suppress vascular inflammatory processes induced by high glucose (HG) in human umbilical vein endothelial cells (HUVECs) and mice. Data showed that HG induced markedly increased vascular permeability, monocyte adhesion, expressions of cell adhesion molecules (CAMs), formation of reactive oxygen species (ROS) and activation of nuclear factor (NF)-B. Remarkably, all of the above mentioned vascular inflammatory effects of HG were attenuated by pretreatment with baicalin, baicalein, and wogonin. Vascular inflammatory responses induced by HG are critical events underlying development of various diabetic complications, therefore, our results suggest that baicalin, baicalein, and wogonin may have significant therapeutic benefits against diabetic complications and atherosclerosis.
Abstract
Vascular inflammatory process has been suggested to play a key role in initiation and progression of atherosclerosis, a major complication of diabetes mellitus. Thus, in this study, we attempted to determine whether three structurally related polyphenols found in the Chinese herb Huang Qui, namely baicalin, baicalein, and wogonin, can suppress vascular inflammatory processes induced by high glucose (HG) in human umbilical vein endothelial cells (HUVECs) and mice. Data showed that HG induced markedly increased vascular permeability, monocyte adhesion, expressions of cell adhesion molecules (CAMs), formation of reactive oxygen species (ROS) and activation of nuclear factor (NF)-B. Remarkably, all of the above mentioned vascular inflammatory effects of HG were attenuated by pretreatment with baicalin, baicalein, and wogonin. Vascular inflammatory responses induced by HG are critical events underlying development of various diabetic complications, therefore, our results suggest that baicalin, baicalein, and wogonin may have significant therapeutic benefits against diabetic complications and atherosclerosis.
참고문헌 (30)
- Whiting DR, "IDF diabetes atlas: global estimates of the prevalence of diabetes for 2011 and 2030", Diabetes Res Clin Pract, 94, pp.311~321 (2011)
- Grundy SM, "Diabetes and cardiovascular disease: a statement for healthcare professionals from the American Heart Association", Circulation, 100, pp.1134~1146 (1999)
- Thomas JE, "The pathophysiology of cardiovascular disease in diabetes mellitus and the future of therapy", J Cardiometab Syndr, 2, pp.108~113 (2007)
- Roglic G, "The burden of mortality attributable to diabetes: realistic estimates for the year 2000", Diabetes Care, 28, pp.2130~2135 (2005)
- Rubino F, "Potential of surgery for curing type 2 diabetes mellitus", Ann Surg, 236, pp.554~559 (2002)
- Li GQ, "Herbal medicines for the management of diabetes", Adv Exp Med Biol, 771, pp.396~413 (2012)
- Day C, "Traditional plant treatments for diabetes mellitus: pharmaceutical foods", Br J Nutr, 80, pp.5~6 (1998)
- Kubo M, "Studies on rehmanniae radix. I. Effect of 50% ethanolic extract from steamed and dried rehmanniae radix on hemorheology in arthritic and thrombosic rats", Biol Pharm Bull, 17, pp.1282~1286 (1994)
- Kubo M, "Studies on Scutellariae radix. VII. Anti-arthritic and anti-inflammatory actions of methanolic extract and flavonoid components from Scutellariae radix", Chem Pharm Bull (Tokyo), 32, pp.2724~2729 (1984)
- Chen YC, "Wogonin, baicalin, and baicalein inhibition of inducible nitric oxide synthase and cyclooxygenase-2 gene expressions induced by nitric oxide synthase inhibitors and lipopolysaccharide", Biochem Pharmacol, 61, pp.1417~1427 (2001)
- Chiu JH, "Tumor necrosis factor-producing activity of wogonin in RAW 264. 7 murine macrophage cell line", Planta Med, 68, pp.1036~1039 (2002)
- Laakso M, "Hyperglycemia and cardiovascular disease in type 2 diabetes", Diabetes, 48, pp.937~942 (1999)
- Kannel WB, "Diabetes and cardiovascular disease. The Framingham study", JAMA, 241, pp.2035~2038 (1979)
- Nannipieri M, "Increased transcapillary escape rate of albumin in microalbuminuric type II diabetic patients", Diabetes Care, 18, pp.1~9 (1995)
- Wardle EN, "Vascular permeability in diabetics and implications for therapy", Diabetes Res Clin Pract, 23, pp.135~139 (1994)
- Tooke JE, "Microvascular function in human diabetes. A physiological perspective", Diabetes, 44, pp.721~726 (1995)
- Gerrity RG, "The role of the monocyte in atherogenesis:I. Transition of blood-borne monocytes into foam cells in fatty lesions", Am J Pathol, 103, pp.181~190 (1981)
- Esposito C, "Long-term exposure to high glucose up-regulates VCAM-induced endothelial cell adhesiveness to PBMC", Kidney Int, 59, pp.1842~1849 (2001)
- Hamuro M, "High glucose induced nuclear factor kappa B mediated inhibition of endothelial cell migration", Atherosclerosis, 162, pp.277~287 (2002)
- Morigi M, "Leukocyteendothelial interaction is augmented by high glucose concentrations and hyperglycemia in a NF-kB-dependent fashion", J Clin Invest, 101, pp.1905~1915 (1998)
- Lopes-Virella MF, "Immune mechanisms of atherosclerosis in diabetes mellitus", Diabetes, 41(2), pp.86~91 (1992)
- 배종섭, "Role of High Mobility Group Box 1 in Inflammatory Disease: Focus on Sepsis", Archives of Pharmacal Research, 35(9), pp.1511~1523 (2012), 대한약학회
- Kado S, "Expression of intercellular adhesion molecule-1 induced by high glucose concentrations in human aortic endothelial cells", Life Sci, 68, pp.727~737 (2001)
- Hansson GK, "The immune response in atherosclerosis: a double-edged sword", Nat Rev Immunol, 6, pp.508~519 (2006)
- Boisvert WA, "Modulation of atherogenesis by chemokines", Trends Cardiovasc Med, 14, pp.161~165 (2004)
- Inoguchi T, "High glucose level and free fatty acid stimulate reactive oxygen species production through protein kinase C--dependent activation of NAD(P)H oxidase in cultured vascular cells", Diabetes, 49, pp.1939~1945 (2000)
- Dunlop M, "Aldose reductase and the role of the polyol pathway in diabetic nephropathy", Kidney Int Suppl, 77, pp.3~12 (2000)
- Han HJ, "High glucose-induced oxidative stress inhibits Na+", glucose cotransporter activity in renal proximal tubule cells, 2005, pp.288, 988~996
- Rimbach G, "Macrophages stimulated with IFN-gamma activate NFkappa B and induce MCP-1 gene expression in primary human endothelial cells", Mol Cell Biol Res Commun, 3, pp.238~242 (2000)
- Uemura S, "Diabetes mellitus enhances vascular matrix metalloproteinase activity:role of oxidative stress", Circ Res, 88, pp.1291~1298 (2001)