GalUR 유전자를 이용한 비타민 C 증대 상추 (Lactuca sativa L.) 형질전환체 개발
Transgenic lettuce (Lactuca sativa L.) with increased vitamin C levels using GalUR gene
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초록
L-Ascorbic acid (vitamin C) in vegetables is an essential component of human nutrition. The objective is to transform lettuce (Lactuca sativa L.) with GalUR gene that is involved in the vitamin C biosynthesis. The cotyledons of Hwoahong (Nongwoo Bio Co.) were used to induce the callus and shoot under the selection media with MS + 30 g/L Sucrose + 0.5 mg/L BAP + 0.1 mg/L NAA + 100 mg/L kanamycin + 200 mg/L lilacillin, pH 5.2. The shoot was developed from the cut side of the explants after 3 weeks on the selection media. We successfully transformed the lettuce with GalUR gene and analyzed the levels of vitamin C. We found that some of the lettuce transgenic lines contained higher levels of vitamin C compared with the normal one (non-transformed). Especially, some of T1 lettuces inserted by GalUR showed about 3~4 times higher content of vitamin C compared to the non-transformed lettuce. This data support the previously work performed with GLOase transgenic T1 lettuces from which several times higher content of vitamin C were identified. The T2 lettuces with high content of vitamin C have been selected for further analysis.
Abstract
L-Ascorbic acid (vitamin C) in vegetables is an essential component of human nutrition. The objective is to transform lettuce (Lactuca sativa L.) with GalUR gene that is involved in the vitamin C biosynthesis. The cotyledons of Hwoahong (Nongwoo Bio Co.) were used to induce the callus and shoot under the selection media with MS + 30 g/L Sucrose + 0.5 mg/L BAP + 0.1 mg/L NAA + 100 mg/L kanamycin + 200 mg/L lilacillin, pH 5.2. The shoot was developed from the cut side of the explants after 3 weeks on the selection media. We successfully transformed the lettuce with GalUR gene and analyzed the levels of vitamin C. We found that some of the lettuce transgenic lines contained higher levels of vitamin C compared with the normal one (non-transformed). Especially, some of T1 lettuces inserted by GalUR showed about 3~4 times higher content of vitamin C compared to the non-transformed lettuce. This data support the previously work performed with GLOase transgenic T1 lettuces from which several times higher content of vitamin C were identified. The T2 lettuces with high content of vitamin C have been selected for further analysis.
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