Self-Incompatibility and Embryo Development in Astragali Radix
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This study was conducted to determine the characteristics of fertilization process and embryo development of Astragalus membranaceus Bunge (Astragali Radix) to provide basic data needed in its breeding. A. membranaceus showed poor seed setting when self-pollination was induced. When artificial pollination was induced, it showed less than 5% bearing in late August, but more than 13% bearing from the beginning of September 4th. The flower size was about 17.0㎜× 4.0 ㎜ and pistils and stamens had the same length of 15.0㎜ at flowering stage. When self-pollination or cross-pollination was induced, pollen tubes extended to an ovule. While pollen tube was extending to the ovule, reproductive cell split and formed two male generative nuclei and a vegetative nucleus. In the case of self-pollination, fertilized embryo was not observed, but was formed in the case of cross-pollination. A. membranaceus is noted to have zygote self-incompatibility. In the case of cross-pollination, fertilization was observed in 6 to 8 h after pollination, where apical cell derivatives split after fertilization. A spherical pro-embryo was then formed three days after fertilization. The seed attained full shape with a seed coat showing its distinctive contour 15 days after fertilization. Thus, A. membranaceus in Leguminosae family is found to have zygote selfincompatibility although its flower shape is shown to match the self-compatibility plant.
Abstract
This study was conducted to determine the characteristics of fertilization process and embryo development of Astragalus membranaceus Bunge (Astragali Radix) to provide basic data needed in its breeding. A. membranaceus showed poor seed setting when self-pollination was induced. When artificial pollination was induced, it showed less than 5% bearing in late August, but more than 13% bearing from the beginning of September 4th. The flower size was about 17.0㎜× 4.0 ㎜ and pistils and stamens had the same length of 15.0㎜ at flowering stage. When self-pollination or cross-pollination was induced, pollen tubes extended to an ovule. While pollen tube was extending to the ovule, reproductive cell split and formed two male generative nuclei and a vegetative nucleus. In the case of self-pollination, fertilized embryo was not observed, but was formed in the case of cross-pollination. A. membranaceus is noted to have zygote self-incompatibility. In the case of cross-pollination, fertilization was observed in 6 to 8 h after pollination, where apical cell derivatives split after fertilization. A spherical pro-embryo was then formed three days after fertilization. The seed attained full shape with a seed coat showing its distinctive contour 15 days after fertilization. Thus, A. membranaceus in Leguminosae family is found to have zygote selfincompatibility although its flower shape is shown to match the self-compatibility plant.
참고문헌 (26)
- Plant embryology, "Bang JW", Plant cytogenetics laboratory Chungnam National University, 75~78 (1999)
- de Nettancourt D, "Incompatibility in angiosperms", Sexual Plant Reproduction, 10, pp.185~199 (1997)
- Fehr WR, "Soybean. In Fehr, W. R. and H. H. Hadley(eds.) Hybridization of crop plant", pp.589~611 (1980), American society of agronomy. Madison Wis
- Franklin FCH, "Cell and molecular biology of self-incompatibility in flowering plants", International Review of Cytology, 158, pp.1~64 (1995)
- Gibbs PE, "Post-pollination events in species of Chorisia (Bombacaceae) and Tabebuia (Bignoniaceae) with lateacting self- incompatibility", Botanica Acta, 106, pp.64~71 (1993)
- Gibbs PE, "Does late-acting self-incompatibility (LSI) show family clustering? Two more species of Bignoniaceae with LSI: Dolichandra cynanchoides and Tabebuia nodosa", Annals of Botany, 84, pp.449~457 (1999)
- Gibbs PE, "Postzygotic control of selfing in Hymenaea stigonocarpa (Leguminosae-Caesalpinioideae), a bat-pollinated tree of the Brazilian cerrados", International Journal of Plant Science, 160, pp.72~78 (1999)
- Hwang JK, "Morphological and embryological studies on the American ginseng(Panax quinquefolium L.)", Jeonbuk University, 9, pp.177~194 (1967)
- Johns CW, "Floral development of a flower structure mutant in soybeans, Glycine max(L) Merr", Amer. J. Bot, 69(5), pp.829~842 (1982)
- Kato I, "Studies on the mechanism of occurrence of abortive grains and their prevention on soybeans, Glycine max", M. Bull. Div. Plant Breed. Cultiv. Tokai-Kinki Natl. Agric. Exp. Stn, 1, pp.115~132 (1954)
- Kaul V, "Early events in the embryo sac after intraspecific and interspecific pollinations in Rhododendron kawakamii and R. retusum", Canadian Journal of Botany, 64, pp.282~291 (1986)
- Kim CM, "Chinese medicinal dictionary", pp.6460~6471 (1998), Jungdam publishing company
- 방재욱, "황기류 식물 3종의 세포유전학적 분석", 한국약용작물학회지, 14(4), pp.250~254 (2006), 한국약용작물학회
- Lewis G, "Reproductive biology of Caesalpinia calycina and C. pluviosa (Leguminoae) of the caatinga of north-eastern Brazil", Plant Systematics and Evolution, 217, pp.43~53 (1999)
- 박희운, "SCAR 마커를 이용한 황기와 몽고황기 판별", 한국약용작물학회지, 15(1), pp.51~55 (2007), 한국약용작물학회
- Masaki A, "Chemical evaluation of Astragali radix", Natural Medicines, 48(4), pp.244~252 (1994)
- Matton DP, "Selfincompatibility: how plants avoid illegitimate offspring", the National Academy of Sciences of the USA91: 1992-1997 (1994)
- Pamplin RA, "The anatomical development of the ovule and seed in the soybean", 박사, Urbana. Diss. Abstr., pp.1963
- Sage TL, "Ovarian and other lateacting self-incompatibility systems. In: Williams EG, Knox RB, Clarke AE, eds. Genetic control of self-incompatibility and reproductive development in flowering plants", pp.116~140 (1994), Dordrecht: Kluwer
- Seavey SR, "Late-acting self-incompatibility in angiosperms", Botanical Review, 52, pp.195~218 (1986)
- Senri J, "Crop experiment methods", 187~225 (1958)
- Soueges R, "Embryogenie des Papilionacees. Developpement de l'embryon chez le Glycine soja Sieb. et Zucc. (Soya hispida Moench)", C. R. Acad. Sci, 229, pp.1183~1185 (1949)
- Tammy LS, "Evidence for Ovarian Selfincompatibility as a Cause of Self-sterility in the Relictual Woody Angiosperm, Pseudowintera axillaris (Winteraceae)", Annals of Botany, 91, pp.807~816 (2003)
- Wang TS, "Chinese Medicinal herb cultivation", pp.691~696 (1988), Agriculture Publishing
- Williams LF, "Structure and genetic characteristics of the soybean. p. 111-134. In K. S. Markery (ed.)" (1950), soybeans and soybean products. Interscience Publishers, New York.
- Williams EG, "Apparent selfincompatibility in Rhododendron ellipticum, R. championae and R. amamiense: a post-zygotic mechanism", Plant Cell Incompatibility Newsletter, 16, pp.10~11 (1984)