Micro-CT 촬영기법에 의한 재배지 참당귀의 뿌리 특성
Study of Root Characteristics of Angelica gigas Nakai in Plantations using a Micro-CT Camera
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Background: We measured the weight characteristics, intercellular space volume as a proportion of root volume, and quantities of the functional component in Angelica gigas Nakai, collected from four cultivation areas in Pyeongchang, including Tapdong-ri and SangjinBu-ri, and tested the results for correlations. Methods and Results: Dry weight was the highest in the Tapdong-ri group (13.37 ± 0.13 g) and the lowest in the SangjinBu-ri group (11.90 ± 0.57 g). The percentage of intercellular spaces within the roots, determined by micro-CT camera, was the highest in the Tapdong-ri group (6.44 ± 0.76%) and the lowest in the SangjinBu-ri group (9.01 ± 2.15%). The quantity of functional components were the highest in the Tapdong-ri group (116.13 ± 9.53 ㎎/g) and the lowest in the SangjinBu-ri group (99.77 ± 23.23 ㎎/g). Conclusions: The dry weight of A. gigas significantly correlated with the volume and percentage of intercellular space. An increase in the dry weight resulted in a negative correlation between the volume and ratio of the intercellular spaces. The volume of intercellular space correlated negatively with levels of the functional components, this was significant in the case of the nodakenin, decursin and decursinol angelate content (p < 0.016).
Abstract
Background: We measured the weight characteristics, intercellular space volume as a proportion of root volume, and quantities of the functional component in Angelica gigas Nakai, collected from four cultivation areas in Pyeongchang, including Tapdong-ri and SangjinBu-ri, and tested the results for correlations. Methods and Results: Dry weight was the highest in the Tapdong-ri group (13.37 ± 0.13 g) and the lowest in the SangjinBu-ri group (11.90 ± 0.57 g). The percentage of intercellular spaces within the roots, determined by micro-CT camera, was the highest in the Tapdong-ri group (6.44 ± 0.76%) and the lowest in the SangjinBu-ri group (9.01 ± 2.15%). The quantity of functional components were the highest in the Tapdong-ri group (116.13 ± 9.53 ㎎/g) and the lowest in the SangjinBu-ri group (99.77 ± 23.23 ㎎/g). Conclusions: The dry weight of A. gigas significantly correlated with the volume and percentage of intercellular space. An increase in the dry weight resulted in a negative correlation between the volume and ratio of the intercellular spaces. The volume of intercellular space correlated negatively with levels of the functional components, this was significant in the case of the nodakenin, decursin and decursinol angelate content (p < 0.016).
참고문헌 (38)
- 박종희, "한국산 Angelica속 식물의 생약학적 연구", 생약학회지, 42(2), pp.103~106 (2011), 한국생약학회
- Buie HR, "Automatic segmentation of cortical and trabecular compartments based on a dual threshold technique for in vivo micro-CT bone analysis", Bone, 41, pp.505~515 (2007)
- Burr-Hersey JE, "Developmental morphology of cover crop species exhibit contrasting behaviour to changes in soil bulk density, revealed by X-ray computed tomography", PLoS ONE, 12, pp.e0181872~ (2017)
- Dejaco A, "Micro CT-based multiscale elasticity of double-porous(pre-cracked) hydroxyapatite granules for regenerative medicine", Journal of Biomechanics, 45, pp.1068~1075 (2012)
- Gainkam LOT, "Comparison of the biodistribution and tumor targeting of two 99mTc-Labeled anti-EGFR nanobodies in mice, using pinhole SPECT", Micro-CT, 2008, pp.49, 788~795
- Analysis of ginseng root growth using neutron radiography and visualization of water distribution in processed ginseng using neutron tomography, "Geumsan Agricultural Technology Service Center", Geumsan Agricultural Technology Service Center, 1~34 (2005)
- 3-dimentional visualization of ginseng root growth at main growth stage in soil using neutron tomography, "Geumsan Ginseng and Medicinal Crop Experiment Station", Geumsan Ginseng and Medicinal Crop Experiment Station, 1~35 (2005)
- 장창석, "한국산 골풀속의 해부학적 형질에 의한 분류학적 연구", 식물분류학회지, 46(4), pp.392~404 (2016), 한국식물분류학회[DOI]
- Jeong HJ, "Diabetic effect of Angelica gigas Nakai leaf extracts in diabetes mellitus type Ⅱ model mice by high fat-fed and streptozotocin-treated rats", Journal of Agricultural, Life and Environmental Sciences, 27, pp.6~14 (2015)
- Jung YM., "The function of nutrient. In the prevention and minerals for Korea agriculture in the future", pp.101~117 (2015), Gomundang printing Inc
- Karahara I, "Three-dimensional imaging of plant tissues using X-ray microcomputed tomography", Plant Morphology, 27, pp.21~26 (2015)
- Kim JW, "Introduction of soil fertilizer", pp.207~213 (2014), Seonjinmunhwasa
- Kim JY, "Angelan isolated from Angelica gigas Nakai induces dendritic cell maturation through toll-like receptor 4", International Immunopharmacology, 7, pp.78~87 (2007)
- 김선애, "참당귀, 중국당귀, 일당귀 및 그 구성 생화합물의 약리작용에 대한 고찰", 대한한의학회지, 32(4), pp.1~24 (2011), 대한한의학회
- 김유아, "참당귀로부터 분리한 Demethylsuberosin의 멜라닌 생성 억제 효과", 생약학회지, 45(3), pp.209~213 (2014), 한국생약학회
- 김영국, "친환경 유기질 비료 시용이 참당귀의 생육과 수량에 미치는 영향", 한국약용작물학회지, 22(2), pp.127~133 (2014), 한국약용작물학회[DOI]
- 권진환, "참당귀 추출분말이 Monosodium Iodoacetate로 유발된 흰쥐의 골관절염에 대한 효과", 분석과학, 28(1), pp.72~77 (2015), 한국분석과학회[DOI]
- Lee MY, "Discrimination of three Angelica species using the RAPDs and internal root structure", Korean Journal of Medical Crop Science, 8, pp.243~249 (2000)
- 이상현, "Antibacterial Coumarins from Angelica gigas Roots", Archives of Pharmacal Research, 26(6), pp.449~452 (2003), 대한약학회
- Lee TB., "Coloured flora of Korea. Vol. 1", pp.839~844 (2003), Hayangmunsa
- Lee YN., "New flora of Korea. Vol. 1", pp.810~832 (2006), Kyohak Publising Co
- Mathers AW, "Investigating the microstructure of plant leaves in 3D with lab-based X-ray computed tomography", Plant Methods, 14, pp.99~ (2018)
- 문영자, "참당귀 추출물을 첨가한 쿠키의 품질 특성", 한국식품영양학회지, 24(2), pp.173~179 (2011), 한국식품영양학회
- 남해인, "당귀 추출물의 궤양성 대장염 억제 효과에 대한 연구", 동의생리병리학회지, 30(6), pp.439~446 (2016), 한의병리학회[DOI]
- Paquita VC, "Monitoring plant growth using high resolution micro-CT images", Proceedings of SPIE-IS&T Electronic Imaging, 7877, pp.78770W~ (2011)
- 박만중, "Streptozotocin 유도 흰쥐에서 참당귀(Angelica gigas Naki) 추출물의 당뇨 개선 효과", 한국식품영양학회지, 22(2), pp.246~251 (2009), 한국식품영양학회
- 박숙경, "참당귀(Angelica gigas Nakai) 뿌리 추출물의 화장품소재 특성", Korean Chemical Engineering Research(HWAHAK KONGHAK), 47(5), pp.553~557 (2009), 한국화학공학회
- 박윤미, "점봉산 참당귀 자생지의 생장 및 유용성분 특성", 한국자원식물학회지, 32(1), pp.9~18 (2019), 한국자원식물학회[DOI]
- Ruzin ES., "Plant microtechnique and morphology", pp.127~128 (1999), Oxford University Press
- Seo BI, "Chinese medicine herbalogy", pp.851~857 (2008), Yeongnimsa
- Sim CM, "Continuous cropping of endangered therapeutic plants via electron beam soil-treatment and neutron tomography", Scientific Report, 8, pp.2136~ (2018)
- Son SH, "Decursin and decursinol inhibit VEGF-induced angiogenesis by blocking the activation of extracellular signal-regulated kinase and c-Jun Nterminal kinase", Cancer Letters, 280, pp.86~92 (2009)
- 송준호, "개나리족, 향선나무족, Myxopyrum속(물푸레나무과) 엽병의 해부학적 형질 및 분류학적 유용성", 식물분류학회지, 42(1), pp.50~63 (2012), 한국식물분류학회
- 성정숙, "당귀의 해부형태학적 특징에 따른 기원판별", 한국약용작물학회지, 12(1), pp.67~72 (2004), 한국약용작물학회
- Torres-Ruiz JM, "Direct X-ray microtomography observation confirms the induction of embolism upon xylem cutting under tension", Journal of Plant Physiology, 167, pp.40~43 (2015)
- Tuan HS, "Application of micro CT and computation modeling in bone tissue engineering", Computer Aided Design, 37, pp.1151~1161 (2005)
- 윤미영, "당귀로부터 분리한 decursinol angelate의 밀 붉은녹병에 대한 항균활성", 식물병 연구, 17(1), pp.25~31 (2011), 한국식물병리학회
- 유홍섭, "참당귀의 정식시기에 따른 생육 및 추대", 한국약용작물학회지, 11(5), pp.392~396 (2003), 한국약용작물학회