GFP gene expression in transfected rainbow trout fibroblast cell line RTG-2 using a polycationic reagent (Superfect)

Abstract

In this study, GFP reporter gene was transfected into a fibroblast cell line RTG2 using a polycationic transfection reagent (Superfect) and showed a successful expression of GFP. The transfection efficiency by Superfect was compared to the commonly used transfection method, i.e. DNA-calcium phosphate coprecipitation. Transfection by Superfect was more effective than calcium phosphate coprecipitation method (frequency of cell expressing GFP was 11.3% and 3.5%, respectively). The optimal expression of GFP and β-galactosidase was observed when 5-6 ㎕ of Superfect per ㎍ DNA was used for transfection, 1:5-6 ratio between DNA (㎍) and Superfect (㎕).