Effects of Plant Growth Regulators on in vitro Propagation of Echinosophora koreensis Nakai
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To establish in vitro nodal culture conditions of Echinosophora koreensis Nakai, one of rare and endangered species famous for beautiful flowers in the Korean Peninsula, the influence of plant growth regulators (PGRs) on shooting and rooting from in vitro shoots was investigated. In shoot multiplication, addition of 6-benzylaminopurine (BA) to the half-strength Driver and Kuniyuki's media in the range of 2.22 to 8.88 µM induced 2.5 to 2.7 shoots per axillary bud; and addition of 2.27 µM thidiazuron (TDZ) produced 3.2 shoots, during 4 weeks of culture, while zeatin and isopentenyl adenine (2ip) were not effective on shoot multiplication as observed from several combination treatments of BA with other PGRs. Shoots established were smaller than 2 cm in length, in most of the treatments. while in BA 8.88 µM treatment more than 30% of shoots were longer than 2 cm and shorter than 4 cm. In rooting, naphthalene acetic acid (NAA) from 5.37 to 21.48 µM showed the rooting rate from 40.0 to 62.5%. Indole butyric acid (IBA) addition had little effect on rooting (＜10%), although some roots in IBA-containing media were longer than those in NAA. Micropropagation from axillary buds of nodular explants was applicable and promising to multiplication and conservation of Echinosophora koreensis Nakai.
Abstract
To establish in vitro nodal culture conditions of Echinosophora koreensis Nakai, one of rare and endangered species famous for beautiful flowers in the Korean Peninsula, the influence of plant growth regulators (PGRs) on shooting and rooting from in vitro shoots was investigated. In shoot multiplication, addition of 6-benzylaminopurine (BA) to the half-strength Driver and Kuniyuki's media in the range of 2.22 to 8.88 µM induced 2.5 to 2.7 shoots per axillary bud; and addition of 2.27 µM thidiazuron (TDZ) produced 3.2 shoots, during 4 weeks of culture, while zeatin and isopentenyl adenine (2ip) were not effective on shoot multiplication as observed from several combination treatments of BA with other PGRs. Shoots established were smaller than 2 cm in length, in most of the treatments. while in BA 8.88 µM treatment more than 30% of shoots were longer than 2 cm and shorter than 4 cm. In rooting, naphthalene acetic acid (NAA) from 5.37 to 21.48 µM showed the rooting rate from 40.0 to 62.5%. Indole butyric acid (IBA) addition had little effect on rooting (＜10%), although some roots in IBA-containing media were longer than those in NAA. Micropropagation from axillary buds of nodular explants was applicable and promising to multiplication and conservation of Echinosophora koreensis Nakai.
참고문헌 (26)
- Ahmed MR, "Role of TDZ in the quick regeneration of multiple shoots from nodal explant of Vitex trifolia L. --an important medicinal plant", Appl Biochem Biotechnol, 168, pp.957~966 (2012)
- Amutha S, "Thidiazuroninduced high-frequency axillary and adventitious shoot regeneration in Vigna radiata(L. )Wilczek", In Vitro Cell Dev Biol-Plant, 42, pp.26~30 (2006)
- Brassard N, "Elongation, rooting and acclimatization of micropropagated shoots from mature material of hybrid larch", PCTOC, 44, pp.37~44 (1996)
- 천경식, "개느삼의 분포와 자생지 환경특성", 식물분류학회지, 39(4), pp.254~263 (2009), 한국식물분류학회
- 최수완, "병풍쌈의 엽병 조직 절편으로부터 식물체 재분화", 한국자원식물학회지, 24(5), pp.483~488 (2011), 한국자원식물학회
- 정재동, "풍란(Neofinetia falcata) 경정 배양에 의한 유묘의 대량 증식", 화훼연구, 12(2), pp.101~106 (2004), 한국화훼학회
- Driver JA, "In vitro propagation of Paradox walnut Juglans hindsii×Juglans regia rootstock", Hort Science, 19, pp.507~509 (1984)
- 한무석, "멸종위기식물 피뿌리풀의 기내증식", Journal of Plant Biotechnology, 31(1), pp.31~35 (2004), 한국식물생명공학회
- Kim CM, "A study on chemical constituents and biological activity of Echinosophora koreensis Nakai", Kor J Pharmacogn, 21, pp.137~141 (1990)
- 김효진, "섬시호(Bupleurum latissimum Nakai)의 조직배양을 통한 대량생산", 한국자원식물학회지, 20(4), pp.367~374 (2007), 한국자원식물학회
- M Kavitha, "In vitro Plant Regeneration from Apical Bud and Nodal Segments of Anthocepahalus Cadamba – An important sacred and medicinal tree", Journal of forest and environmental science, 25(2), pp.111~118 (2009), 산림과학연구소
- Khalafalla MM, "A combination of thidiazuron and benzyladenine promotes nultiple shoot production from cotyledonary node explants of faba bean(Vicia faba L. )", Plant Growth Regulation, 27, pp.145~148 (1999)
- Khan MI, "Modulation of in vitro morphogenesis in nodal segments of Salix tetrasperma Roxb. through the use of TDZ, different media types and culture regimes", Agrofor Syst, 86, pp.95~103 (2012)
- 고갑천, "한국 특산 히어리의 기내 증식", 원예과학기술지, 24(2), pp.272~278 (2006), 한국원예학회
- Nitish kumar, "In vitro Plant Propagation: A Review", Journal of forest and environmental science, 27(2), pp.61~72 (2011), 산림과학연구소
- 이종석, "자생 산수국(Hydrangea serrata)의 삽목시기와 IBA, NAA, Rooton이 발근에 미치는 영향", 한국인간식물환경학회지, 10(3), pp.131~134 (2007), 한국인간식물환경학회
- 이준구, "발근촉진제 및 근권 온도조절이 하이부시 블루베리 삽수의 생육 및 발근에 미치는 영향", 원예과학기술지, 27(1), pp.7~11 (2009), 한국원예학회
- The study of growth characteristic of nation's major plants and best management practicestrees and shrubs, "Ministory of Environment", 274~275 (1997)
- 문흥규, "희귀 및 멸종위기 수종 개느삼의 액아배양을 통한 기내번식", Journal of Plant Biotechnology, 35(3), pp.229~234 (2008), 한국식물생명공학회
- 문흥규, "멸종위기 식물 덩굴용담의 기내번식에 미치는 생장조절제 효과", Journal of Plant Biotechnology, 36(1), pp.13~17 (2009), 한국식물생명공학회
- Mukhtar S, "Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types", Physiol Mol Biol Plants, 18, pp.381~386 (2012)
- 오병운, "강원도 양구군 특산식물 개느삼(콩과) 자생지 내의 식물상과 식생 -양구군 남면 죽곡리, 황강리를 중심으로-", 한국환경복원기술학회지, 12(2), pp.19~28 (2009), 한국환경복원기술학회
- 박상규, "고추냉이 정단배양에 있어서 BA 농도 및 배양방법에 따른 기내증식 효과", Journal of Plant Biotechnology, 34(1), pp.1~6 (2007), 한국식물생명공학회
- 심경구, "造景用 鄕土樹種 利用을 위한 素材開發에 관한 硏究-韓國 特産植物 개느삼(Echinosophora koreensis Nakai)의 自生地 調査 및 形態的 特性에 관하여-", 한국전통조경학회지, 24(1), pp.32~42 (2006), 한국전통조경학회
- Valero-Aracama C, "Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult-and easy-to-acclimatize sea oats(Uniola paniculata L. )genotypes", Plant Growth Regulation, 60, pp.43~49 (2010)
- "Discovered Echinosophora koreensis Nakai. which is one of the Korea endemic species in Jiri mountain by Sung, Hwan-Gil" (2010)[URL]