의이인 추출물이 알레르기 비염 동물의 cytokine에 미치는 영향
The Effects of Coisis semen extract on blood serum cytokine of Allergic Rhinitis Mice model
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Objective : Allergic rhinitis(AR) is an inflammation or irritaion of nasal mucosa. Usually, serum cytokine is regarded as one of the most important factors in AR. Coisis semen have been used for the diseases in digestive organs in traditional chinese medicine. Nowaday, there were some studies reported about the effects of coisis semens on serum cytokine. However the results of theses studies were varies, and moreover there was no study which have used AR animal model. Therefore this study is aimed to determine therapeutic effects of coisis semen extract by observing changes of serum cytokine(IgE, TNF-α, IL-1β, IFN-γ and IL-13) with AR animal model. Methods : Thirty BALB/c mice were divided into six groups : Normal group, Control group, sample Ⅰ, sample Ⅱ, sample Ⅲ, sample Ⅳ. Except for Normal group, all the mice in other 5 groups were sensitized intraperionealy by 0.1% ovalbumin solution three times at intervals of one week. Then intranasal sensitization was performed by diffusing 0.1% ovalbumin solution three times at intervals of two days. Normal group were used Normal Saline instead of ovalbumin solution. After the first day of study, sample Ⅰ, sample Ⅱ, sample Ⅲ, and sample Ⅳ were orally administrated coisis semen extract by 100㎎/㎏, 200㎎/㎏, 500㎎/㎏, and 1g/㎏ respectively for 28days. Then, the changes of serum IgE,TNF-α, IL-1β, IFN-γ and IL-13 were observed in 6 groups. We used the statistical methods of ANOVA , post hoc by duncun, and Kruskal-Wallis test(p<0.05)Results : There were statistical changes in IgE and TNF-α. But, there were no statistical changes in IL-1β, IL-13and IFN-γ. Conclusions : According to above results, it is supposed that coisis semen extract has not some significant effects on cytokine of AR animal model. There was no evidence for using coisis semen in relieving symptoms of allergic rhinitis.
Abstract
Objective : Allergic rhinitis(AR) is an inflammation or irritaion of nasal mucosa. Usually, serum cytokine is regarded as one of the most important factors in AR. Coisis semen have been used for the diseases in digestive organs in traditional chinese medicine. Nowaday, there were some studies reported about the effects of coisis semens on serum cytokine. However the results of theses studies were varies, and moreover there was no study which have used AR animal model. Therefore this study is aimed to determine therapeutic effects of coisis semen extract by observing changes of serum cytokine(IgE, TNF-α, IL-1β, IFN-γ and IL-13) with AR animal model. Methods : Thirty BALB/c mice were divided into six groups : Normal group, Control group, sample Ⅰ, sample Ⅱ, sample Ⅲ, sample Ⅳ. Except for Normal group, all the mice in other 5 groups were sensitized intraperionealy by 0.1% ovalbumin solution three times at intervals of one week. Then intranasal sensitization was performed by diffusing 0.1% ovalbumin solution three times at intervals of two days. Normal group were used Normal Saline instead of ovalbumin solution. After the first day of study, sample Ⅰ, sample Ⅱ, sample Ⅲ, and sample Ⅳ were orally administrated coisis semen extract by 100㎎/㎏, 200㎎/㎏, 500㎎/㎏, and 1g/㎏ respectively for 28days. Then, the changes of serum IgE,TNF-α, IL-1β, IFN-γ and IL-13 were observed in 6 groups. We used the statistical methods of ANOVA , post hoc by duncun, and Kruskal-Wallis test(p<0.05)Results : There were statistical changes in IgE and TNF-α. But, there were no statistical changes in IL-1β, IL-13and IFN-γ. Conclusions : According to above results, it is supposed that coisis semen extract has not some significant effects on cytokine of AR animal model. There was no evidence for using coisis semen in relieving symptoms of allergic rhinitis.
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