감마선 조사 처리에 의한 결명자 줄기의 전처리와 효소가수분해 효과
Effect of Gamma Ray Irradiation on the Pretreatment and Enzymatic Hydrolysis of Senna tora Stalk
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: The demand of recycling renewable agricultural by-products is increasing. Radiation breeding is a method used to improve saccharification efficiency. Thus, we investigated the effect of gamma ray irradiation on the pretreatment and enzymatic hydrolysis of the stalks of Senna tora, an important medicinal plants. Methods and Results: S. tora seeds were irradiated with gamma ray at doses of 100, 200, 300, and 400 Gy. In the pretreated biomass, glucan and lignin content were higher in the M1 (1st generations of irradiation) S. tora stalks than in the M2 (2nd generations of irradiation) stalks, this can be explained by the higher degradation rate in M1. After oxalic acid pretreatment, the concentration of total phenolic compounds (TPCs) in the hydrolysate increased in the gamma ray treated seeds. The highest relative increase rate in crystallinity in the pretreated biomass was observed in M1-400 Gy and M2-100 Gy. The cellulose conversion rate was higher in M1 than in M2, except for 200 Gy. Conclusions: Gamma ray irradiation at an appropriate dose can be used to improve the efficiency of pretreatment and enzymatic hydrolysis, thereby increasing biomass availability.
Abstract
Background: The demand of recycling renewable agricultural by-products is increasing. Radiation breeding is a method used to improve saccharification efficiency. Thus, we investigated the effect of gamma ray irradiation on the pretreatment and enzymatic hydrolysis of the stalks of Senna tora, an important medicinal plants. Methods and Results: S. tora seeds were irradiated with gamma ray at doses of 100, 200, 300, and 400 Gy. In the pretreated biomass, glucan and lignin content were higher in the M1 (1st generations of irradiation) S. tora stalks than in the M2 (2nd generations of irradiation) stalks, this can be explained by the higher degradation rate in M1. After oxalic acid pretreatment, the concentration of total phenolic compounds (TPCs) in the hydrolysate increased in the gamma ray treated seeds. The highest relative increase rate in crystallinity in the pretreated biomass was observed in M1-400 Gy and M2-100 Gy. The cellulose conversion rate was higher in M1 than in M2, except for 200 Gy. Conclusions: Gamma ray irradiation at an appropriate dose can be used to improve the efficiency of pretreatment and enzymatic hydrolysis, thereby increasing biomass availability.
참고문헌 (27)
- Berlin A, "Inhibition of cellulase, xylanase and <TEX>${\beta}$<", TEX>-glucosidase activities by softwood lignin preparations, 2006, pp.125, 198~209
- Binod P, "Bioethanol production from rice straw: An overview", Bioresource Technology, 101, pp.4767~4774 (2010)[DOI]
- Castro E, "Dilute acid pretreatment of rapeseed straw for fermentable sugar generation", Bioresource Technology, 102, pp.1270~1276 (2011)[DOI]
- Gupta R, "Separate hydrolysis and fermentation(SHF) of Prosopis juliflora, a woody substrate, for the production of cellulosic ethanol by Saccharomyces cerevisiae and Pichia stipitis-NCIM 3498", Bioresource Technology, 100, pp.1214~1220 (2009)[DOI]
- 장수경, "약산 전처리 조건에 따른 소경 신갈나무 유래 푸르푸랄 및 레불린산의 함량 변화", 목재공학, 43(6), pp.838~850 (2015), 한국목재공학회
- Jeong SY, "Improvement of the fermentability of oxalic acid hydrolysates by detoxification using electrodialysis and adsorption", Bioresource Technology, 152, pp.444~449 (2014)[DOI]
- 정재영, "목질계 바이오매스 전처리 공정에서 발생하는 리그닌 부산물 활용 기술 개발 동향", 공업화학, 27(2), pp.135~144 (2016), 한국공업화학회
- 김동휘, "황기 발아 및 생장에 미치는 감마선 조사 효과", 한국약용작물학회지, 16(4), pp.238~241 (2008), 한국약용작물학회
- 김혜연, "바이오에탄올 생산을 위한 백합나무(Liriodendron tulipifera) 칩의 동시당화발효 및 Response Surface Method를 이용한 옥살산 전처리 조건 탐색", 목재공학, 39(1), pp.75~85 (2011), 한국목재공학회
- 김혜연, "바이오에탄올 생산에 적합한 백합나무 (Liriodendron tulipifera)의 oxalic acid 전처리 효과 탐색", 목재공학, 37(4), pp.397~405 (2009), 한국목재공학회
- 김수미, "NaOH를 이용한 우드칩의 당화 전처리에 대한 감마선 조사 영향 연구", Korean Chemical Engineering Research(HWAHAK KONGHAK), 54(3), pp.431~435 (2016), 한국화학공학회
- Kootstra AMJ, "Comparison of dilute mineral and organic acid pretreatment for enzymatic hydrolysis of wheat straw", Biochemical Engineering Journal, 46, pp.126~131 (2009)[DOI]
- Korean Statistical Information Service(KOSIS), "Special crop production status" (2017)
- 이충건, "한국의 에너지용 바이오매스활용을 위한 농업부산물 잠재에너지량 추정 및 한국형 수거모델에 관한 연구", 신재생에너지, 13(1), pp.27~35 (2017), 한국신·재생에너지학회
- Lee JW, "Simultaneous saccharification and ethanol fermentation of oxalic acid pretreated corncob assessed with response surface methodology", Bioresource Technology, 100, pp.6307~6311 (2009)[DOI]
- 임정대, "한국산 약용식물로부터의 SOD 활성 및 Phenolic Compounds 함량 비교", 한국약용작물학회지, 12(3), pp.191~202 (2004), 한국약용작물학회
- Mansouri A, "Production of bioethanol from a mixture of agricultural feedstocks: Biofuels characterization", Fuel, 185, pp.612~621 (2016)[DOI]
- Roncero MB, "The effect of xylanase on lignocellulosic components during the bleaching of wood pulps", Bioresource Technology, 96, pp.21~30 (2005)[DOI]
- 류정석, "열병합 발전소용 목질계 바이오매스의 연소 특성에 관한 연구", 공업화학, 22(3), pp.296~300 (2011), 한국공업화학회
- Scordia D, "Effectiveness of dilute oxalic acid pretreatment of Miscanthus<TEX>$\times$<", TEX>giganteus biomass for ethanol production, 2013, pp.59, 540~548
- Singleton VL, "Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent", Methods in Enzymology, 299, pp.152~178 (1999)
- Sluiter A, "Determination of structural carbohydrates and lignin in biomass: Laboratory analytical procedure" (2008)
- Sun SN, "Characteristics and enzymatic hydrolysis of cellulose-rich fractions from steam exploded and sequentially alkali delignified bamboo(Phyllostachys pubescens)", Bioresource Technology, 163, pp.377~380 (2014)[DOI]
- Xin LZ, "Effect of radiation pretreatment on enzymatic hydrolysis of rice straw with low concentrations of alkali solution", Bioresource Technology, 43, pp.13~17 (1993)[DOI]
- Yang S, "Lignocellulosic structural changes of Spartina alterniflora after anaerobic monoand co-digestion", International Biodeterioration and Biodegradation, 63, pp.569~575 (2009)[DOI]
- Zhang YHP, "Toward and aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems", Biotechnology and Bioengineering, 88, pp.797~824 (2004)[DOI]
- Zhao L, "Influence of various glucose", xylose mixtures on ethanol production by Pachysolen tannophilus, 2008, pp.32, 1156~1161