紅蔘藥鍼液의 胃癌細胞柱 遺傳子 發顯에 미치는 影響
Effect of Ginseng Radix Rubra Herbal-acupuncture Solution(GRR-HAS) on Gene Expression in SNU484 carcinomar cells
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Abstract
Objective: It has long been known about the anticancer effect of GRR-HAS, however, it has not been systemically determined the differentially regulated genes by GRR-HAS in cancer cells. The purpose of this study is to screen the GRR-HAS mediated differentially expressed genes in cancer cells such as SNU484 gastric cancer cell lines. Oligonucleotide microarray approache was employed to screen the differential expression genes. Methods: GRR-HAS was prepared by boiling and stored at -70℃ until use. Cells were treated with various concentrations of GRR-HAS(0.1, 0.5, 1.5, 10, 20㎎/㎖) for 24 h. Cell toxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with 1.5㎎/㎖ of GRR-HAS. For oligonucleotide microarray assay, total RNA was used for gene expression analysis using oligonucleotide Genechip (Human genome U133 Plus 2.0., Affimatrix Co.). Results: It has no cytotoxic effects on both HepG2 and SNU484 cells in all concentrations(0.1, 0.5, 1.5, 10, 20㎎/㎖). In oligonucleotide microarray assay, in SNU484 cells, the number of more than twofold up-regulated genes was 346. The number of more than twofold down-regulated genes was 9. Discussion: This study showed the comprehensive gene expression analysis using oligonucleotide microarray for the screening of GRR-HAS mediated differentially regulated genes. These results will provide a better application of GRR-HAS in cancer field and drug target development.
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