Sinuolinea capsularis (Myxosporea: Sinuolineidae) Isolated from Urinary Bladder of Cultured Olive Flounder Paralichthys olivaceus
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Sinuolinea capsularis Davis, 1917 is myxosporean that infect the urinary system of the host fish. Insufficient mor- phological and molecular data of S. capsularis exits, and it is therefore difficult to make an accurate identification of the parasite. We tried a series of morphological and molecular analysis to identify an myxosporean isolated from urinary blad- der of cultured olive flounder, Paralichthys olivaceus, from Jeju island in the Republic of Korea. Some of them were ob- served under a light microscope and SEM, and remain samples were used molecular and phylogenetic analysis. Mature spores were subspherical, measuring 13.9±0.6 μm in length and 13.8±0.8 μm in width. Two spherical polar capsules on opposite sides in the middle of the spore had a diameter range of 4.3±0.4 μm. Scanning electron microscopy revealed that spores a severely twisted the suture line. By the morphological comparison and analysis, it was identified as S. capsu- laris. In addition, we obtained the partial 18S rDNA of S. capsularis and first registered it in NCBI. Phylogenetic analysis showed that S. capsularis clustered with Zschokkella subclade infecting the urinary system of marine fish, and it supported the infection site tropism effect on phylogeny of marine myxosporeans as well as the origin of Sinuolinea is not monophyly.
Abstract
Sinuolinea capsularis Davis, 1917 is myxosporean that infect the urinary system of the host fish. Insufficient mor- phological and molecular data of S. capsularis exits, and it is therefore difficult to make an accurate identification of the parasite. We tried a series of morphological and molecular analysis to identify an myxosporean isolated from urinary blad- der of cultured olive flounder, Paralichthys olivaceus, from Jeju island in the Republic of Korea. Some of them were ob- served under a light microscope and SEM, and remain samples were used molecular and phylogenetic analysis. Mature spores were subspherical, measuring 13.9±0.6 μm in length and 13.8±0.8 μm in width. Two spherical polar capsules on opposite sides in the middle of the spore had a diameter range of 4.3±0.4 μm. Scanning electron microscopy revealed that spores a severely twisted the suture line. By the morphological comparison and analysis, it was identified as S. capsu- laris. In addition, we obtained the partial 18S rDNA of S. capsularis and first registered it in NCBI. Phylogenetic analysis showed that S. capsularis clustered with Zschokkella subclade infecting the urinary system of marine fish, and it supported the infection site tropism effect on phylogeny of marine myxosporeans as well as the origin of Sinuolinea is not monophyly.
참고문헌 (39)
- Lom J, "Myxozoan genera : definition and notes on taxonomy, life-cycle terminology and pathogenic species", Folia Parasitol, 53, pp.1~36 (2006)
- Bartošová P, "Sphaerospora sensu stricto : taxonomy, diversity and evolution of a unique lineage of myxosporeans(Myxozoa)", Mol Phylogenet Evol, 68, pp.93~105 (2013)
- Davis HS, "The Myxosporidia of the Beaufort region : a systematic and biologic study", Bull Bur Fish, 35, pp.201~243 (1917)
- Vaz Rodrigues M, "Sinuolinea niloticus n. sp., a myxozoan parasite that causes disease in Nile tilapia (Oreochromis niloticus)", Parasitol Res, 115, pp.4307~4316 (2016)
- Garner MM, "Renal myxozoanosis in weedy sea dragons, Phyllopteryx taeniolatus (Lacepède), caused by Sinuolinea phyllopteryxa n. sp", J Fish Dis, 31, pp.27~35 (2018)
- Kawai T, "Identification of Kudoa septempunctata as the causative agent of novel food poisoning outbreaks in Japan by consumption of Paralichthys olivaceus in raw fish", Clin Infect Dis, 54, pp.1046~1052 (2012)
- 송준영, "2012년 제주의 양식 넙치 및 자연산 어류의 Kudoa septempunctata 감염 조사", 한국어병학회지, 26(3), pp.129~137 (2013), 한국어병학회[DOI]
- 김승민, "제주도 여윔증상 넙치(Paralichthys olivaceus)로부터 분리한 점액포자충의 특성 분석", 한국수산과학회지, 48(3), pp.337~345 (2015), 한국수산과학회
- Sekiya M, "Enteromyxum leei(Myxosporea : Bivalvulida)as the cause of myxosporean emaciation disease of farmed olive flounders(Paralichthys olivaceus)and a turbot(Scophthalmus maximus)on Jeju Island, Korea", Parasitol Res, 115, pp.4229~4237 (2016)
- Shin SP, "Molecular diagnostics for verifying an etiological agent of emaciation disease in cultured olive flounder Paralichthys olivaceus in Korea", Aquaculture, 493, pp.18~25 (2018)
- Lom J, "A guideline for the preparation of species descriptions in Myxosporea", J Fish Dis, 12, pp.151~156 (1989)
- Barta JR, "Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences", J Parasitol, 83, pp.262~271 (1997)
- Fonseca VG, "Second-generation environmental sequencing unmasks marine metazoan biodiversity", Nat Commun, 1, pp.98~ (2010)
- Larkin MA, "Clustal W and Clustal X version 2.0", Bioinformatics, 23, pp.2947~2948 (2007)
- Kumar S, "MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets", Mol Biol Evol, 33, pp.1870~1874 (2016)
- Sievers F, "Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega", Mol Syst Biol, 7, pp.539~ (2011)
- Guindon S, "A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood", Syst Biol, 52, pp.696~704 (2003)
- Darriba D, "jModelTest 2 : more models, new heuristics and parallel computing", Nat Methods, 9, pp.772~ (2012)
- Ronquist F, "MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space", Syst Biol, 61, pp.539~542 (2012)
- Basikalowa A, "Wissenschaftliches Zentralinstitut für Fischereiwirtschaft", 136~ (1932)
- Tripathi YR, "Some new Myxosporidia from Plymouth with a proposed new classification of the order", Parasitology, 39, pp.110~118 (1948)
- Zhao Y, "Studies on the morphology and taxonomy of three new myxosporeans of the genus Sinuolinea Davis, 1917(Myxosporea : Sinuolineidae)infecting the urinary bladder of some marine fishes from the Shandong coast, China", Syst Parasitol, 55, pp.53~59 (2003)
- Moran JDW, "A review of the myxosporean genus Kudoa Meglitsch, 1947, and its impact on the international aquaculture industry and commercial fisheries", Aquaculture, 172, pp.163~196 (1999)
- Sitjà-Bobadilla A, "Fish Parasites: Pathobiology and Protection", pp.163~176 (2012), CABI
- Whipps CM, "Phylogeography of the cosmopolitan marine parasite Kudoa thyrsites(Myxozoa : Myxosporea)", J Eukaryot Microbiol, 53, pp.364~373 (2006)
- Burger MA, "Low host specificity in the Kudoidae(Myxosporea : Multivalvulida)including seventeen new host records for Kudoa thalassomi", Folia Parasitol, 58, pp.1~16 (2011)
- Yanagida T, "Myxosporean emaciation disease", Fish Pathol, 52, pp.63~67 (2017)
- Shin SP, "Parvicapsula curvatura n. sp. in cultured olive flounder Paralichthys olivaceus and phylogenetic characteristics of Parvicapsula genus", Dis Aquat Organ, 130, pp.199~207 (2018)
- Hillis DM, "Ribosomal DNA: molecular evolution and phylogenetic inference", Q Rev Biol, 66, pp.411~453 (1991)
- Bartosová P, "Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans(Myxozoa : Myxosporea)and provide an effective tool for their molecular phylogenetics", Mol Phylogenet Evol, 53, pp.81~93 (2009)
- Dyková I, "Sinuolinea infections in the urinary system of Cynoscion species(Sciaenidae)and phylogenetic position of the type species of Sinuolinea Davis, 1917(Myxozoa : Myxosporea)", Int J Parasitol Parasites Wildl, 2, pp.10~17 (2013)
- Blaxter ML, "A molecular evolutionary framework for the phylum Nematoda", Nature, 392, pp.71~75 (1998)
- Fiala I, "Evolutionary origin of Ceratonova shasta and phylogeny of the marine myxosporean lineage", Mol Phylogenet Evol, 86, pp.75~89 (2015)
- Bartošová P, "Phylogenetic position of Sphaerospora testicularis and Latyspora scomberomori n. gen. n. sp. (Myxozoa) within the marine urinary clade", Parasitology, 138, pp.381~393 (2011)
- Holzer AS, "Molecular relationships and phylogeny in a community of myxosporeans and actinosporeans based on their 18S rDNA sequences", Int J Parasitol, 34, pp.1099~1111 (2004)
- Fiala I, "The phylogeny of Myxosporea(Myxozoa)based on small subunit ribosomal RNA gene analysis", Int J Parasitol, 36, pp.1521~1534 (2006)
- Shin SP, "The phylogenetic study on Thelohanellus species(Myxosporea)in relation to host specificity and infection site tropism", Mol Phylogenet Evol, 72, pp.31~34 (2014)
- Shin SP, "Phylogenetic study of the genus Kudoa (Myxozoa: Multivalvulida) with a description of Kudoa rayformis sp. nov. from the trunk muscle of Pacific sierra Scomberomorus sierra", Mol Phylogenet Evol, 98, pp.337~345 (2016)
- Kent ML, "Recent advances in our knowledge of the Myxozoa", J Eukaryot Microbiol, 48, pp.395~413 (2001)