Multiomics reveals changes in lipid metabolism in the livers of Landes geese before and after overfeeding
Multiomics reveals changes in lipid metabolism in the livers of Landes geese before and after overfeeding
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective: The aim of this experiment was to integrate production indices with omics sequencing to elucidate the systemic perturbations between hepatic metabolism and the gut microbiota during overfeeding.Methods: A total of 120 seven-week-old male Landes geese were floor-reared in a pen environment. Overfeeding commenced at week 8 using a corn-based diet containing 5% soybean oil. The feeding regimen consisted of three daily meals (150–180 g/meal) initially, gradually increasing to five meals (300–500 g/meal) after two weeks, and was maintained for a total overfeeding period of four weeks.Results: The results demonstrated that overfeeding significantly increased liver weight and serum lipid levels, accompanied by intracellular lipid droplet accumulation. Concurrently, the downregulation of taurine-conjugated bile acids and the upregulation of free bile acids disrupted cholesterol homeostasis. Crucially, overfeeding triggered gut microbial dysbiosis characterized by Escherichia–Shigella enrichment and norank_o_Clostridia_UCG-014 depletion.Conclusion: Our work demonstrated that the identification of the "gut microbiota‒bile acid‒liver axis" could serve as a pivotal signaling pathway driving overfeeding-induced foie gras formation while providing a theoretical foundation for overfeeding strategies to mitigate metabolic pathologies in waterfowl production.
Abstract
Objective: The aim of this experiment was to integrate production indices with omics sequencing to elucidate the systemic perturbations between hepatic metabolism and the gut microbiota during overfeeding. Methods: A total of 120 seven-week-old male Landes geese were floor-reared in a pen environment. Overfeeding commenced at week 8 using a corn-based diet containing 5% soybean oil. The feeding regimen consisted of three daily meals (150–180 g/meal) initially, gradually increasing to five meals (300–500 g/meal) after two weeks, and was maintained for a total overfeeding period of four weeks. Results: The results demonstrated that overfeeding significantly increased liver weight and serum lipid levels, accompanied by intracellular lipid droplet accumulation. Concurrently, the downregulation of taurine-conjugated bile acids and the upregulation of free bile acids disrupted cholesterol homeostasis. Crucially, overfeeding triggered gut microbial dysbiosis characterized by Escherichia–Shigella enrichment and norank_o_Clostridia_UCG-014 depletion. Conclusion: Our work demonstrated that the identification of the "gut microbiota‒bile acid‒liver axis" could serve as a pivotal signaling pathway driving overfeeding-induced foie gras formation while providing a theoretical foundation for overfeeding strategies to mitigate metabolic pathologies in waterfowl production.