Effects of repeated tuberculin skin testings on immune responses in experimental monkeys
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Though many alternative methods to tuberculin skin testing (TST) have been established andevaluated in recent years, sensitivities and specificities of most methods could not meet therequirements of golden standards. In this study, we sought to identify whether repeated TSTscould affect the immune responses in experimental monkeys. Nine natural tuberculosis (TB)monkeys receiving repeated TSTs biweekly were used to demonstrate the effect on TSTresponsiveness. Two healthy monkeys were administrated with repeated TSTs to analyze theimmune response profiling. Intrapalpebral reactions in TB infections gradually weakened orpresented intermittent positive reactions. The leukocyte counts, cytokine responses, andantibody responses to all antigens except Old tuberculin (OT) and MPT64L showed nospecific changes for TB in healthy monkeys. Positive antibody responses to OT and MPT64Lemerged during the first half experimental period, which may cause by their cross-reactivitywith mycobacterial species. Results showed that repeated TSTs had no significant effects onimmune responses in healthy monkeys but a progressive reduction in TST responsiveness inTB infections.
Abstract
Though many alternative methods to tuberculin skin testing (TST) have been established andevaluated in recent years, sensitivities and specificities of most methods could not meet therequirements of golden standards. In this study, we sought to identify whether repeated TSTscould affect the immune responses in experimental monkeys. Nine natural tuberculosis (TB)monkeys receiving repeated TSTs biweekly were used to demonstrate the effect on TSTresponsiveness. Two healthy monkeys were administrated with repeated TSTs to analyze theimmune response profiling. Intrapalpebral reactions in TB infections gradually weakened orpresented intermittent positive reactions. The leukocyte counts, cytokine responses, andantibody responses to all antigens except Old tuberculin (OT) and MPT64L showed nospecific changes for TB in healthy monkeys. Positive antibody responses to OT and MPT64Lemerged during the first half experimental period, which may cause by their cross-reactivitywith mycobacterial species. Results showed that repeated TSTs had no significant effects onimmune responses in healthy monkeys but a progressive reduction in TST responsiveness inTB infections.
참고문헌 (11)
- Centers for Disease Control and Prevention, "Tuberculosis in imported nonhuman primates: United States, June 1990 May 1993", MMWR Morb Mortal Wkly Rep, 42, pp.572~576 (1993)
- McMurray DN, "A nonhuman primate model for preclinical testing of new tuberculosis vaccines", Clin Infect Dis, 30(3), pp.S210~S212 (2000)
- Lerche NW, "New approaches to tuberculosis surveillance in nonhuman primates", ILAR J, 49(2), pp.170~178 (2008)
- Ryan TJ, "An evaluation of the gamma interferon test for detecting Bovine tuberculosis in cattle 8 to 28 days after tuberculin skin testing", Res Vet Sei, 69(1), pp.57~61 (2000)
- National Research Council, "Guide for the Care and Use of Laboratory Animals" (2010), National Academy Press
- Capuano SV, "Experimental Mycobacterium tuberculosis infection of cynomolgus macaques closely resembles the various manifestations of human", M Tuberculosis Infection Infect Immun, 71(10), pp.5831~5844 (2003)
- Min FG, "Serum antibody responses to 10 Mycobacterium tuberculosis proteins, purified protein derivative, and old tuberculin in natural and experimental tuberculosis in rhesus monkeys", Clin Vaccine Immunol, 18(12), pp.2154~2160 (2011)
- Min FG, "Mycobacterium tuberculosis infection in rhesus monkeys (Macaca mulatta) and evaluation of ESAT-6 and CFP10 as immunodiagnostic antigens", Exp Anim, 62(4), pp.281~293 (2013)
- Smithburn KC, "Haematological studies in experimental tuberculosis: variations in the blood cells of rabbits inoculated with cultures differing in virulence", American Review of Tuberculosis, XXXVI, pp.673~691 (1937)
- Harboe M, "Antigen of PPD, old tuberculin and autoclaved mycobacterium bovis BCG studied by Immunoelectrophoresis", Am Rev Respir Dis, 124, pp.80~87 (1981)
- Weldingh K, "Assessing the serodiagnostic potential of 35 Mycobacterium tuberculosis proteins and identification of four novel serological antigens", J Clin Microbiol, 43(1), pp.57~65 (2005)