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Effect of dietary mannanoligosaccharide supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs

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Background: Use of prebiotics in companion animal nutrition is often considered advantageous over probiotics because of the ease of handling, ability to withstand processing and storage etc. While most of the studies on prebiotic use in dogs have been done with processed food as basal diet, the response in relation to homemade diet feeding is not very well explored. Methods: The study was conducted to evaluate the effects of dietary mannanoligosaccharide (MOS) supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs. Ten Spitz pups were divided into two groups: control (CON) with no supplementation, and experimental (MOS) wherein the basal diet was supplemented with MOS at 15 g/kg diet. All dogs were fed on a home-prepared diet for a period of 150 days. The study protocol included a digestion trial, periodic blood collection and analysis for lipid profile and erythrocytic antioxidants. Immune response of the animals was assessed towards the end of the feeding period. Results: Results revealed no significant (P > 0.05) variations in palatability score, intake and apparent digestibility of nutrients between the groups. Faecal score, faeces voided, faecal pH, concentrations of ammonia, lactate and short-chain fatty acids were comparable (P > 0.05) between the two groups. Cell-mediated immune response, assessed as delayed-type of hypersensitivity response, was significantly higher (P < 0.05) in the MOS group. The percent of lymphocyte sub-populations CD4+ and ratio of CD4+:CD8+ were also significantly (P < 0.05) higher in MOS group. The serum IgG levels were similar (P > 0.05) in both the groups. Supplementation of MOS lowered (P < 0.05) serum total- and LDL- cholesterol levels, when compared with the control group. The erythrocytic antioxidant indices were similar (P > 0.05) between the two groups. Conclusions: The results indicated that supplementation of MOS at the rate of 15 g/kg in the diet of dog augmented the cell-mediated immune response and serum lipid profile without any influences on digestibility of nutrients, hindgut fermentation and antioxidants indices.

Abstract

Background: Use of prebiotics in companion animal nutrition is often considered advantageous over probiotics because of the ease of handling, ability to withstand processing and storage etc. While most of the studies on prebiotic use in dogs have been done with processed food as basal diet, the response in relation to homemade diet feeding is not very well explored. Methods: The study was conducted to evaluate the effects of dietary mannanoligosaccharide (MOS) supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs. Ten Spitz pups were divided into two groups: control (CON) with no supplementation, and experimental (MOS) wherein the basal diet was supplemented with MOS at 15 g/kg diet. All dogs were fed on a home-prepared diet for a period of 150 days. The study protocol included a digestion trial, periodic blood collection and analysis for lipid profile and erythrocytic antioxidants. Immune response of the animals was assessed towards the end of the feeding period. Results: Results revealed no significant (P > 0.05) variations in palatability score, intake and apparent digestibility of nutrients between the groups. Faecal score, faeces voided, faecal pH, concentrations of ammonia, lactate and short-chain fatty acids were comparable (P > 0.05) between the two groups. Cell-mediated immune response, assessed as delayed-type of hypersensitivity response, was significantly higher (P < 0.05) in the MOS group. The percent of lymphocyte sub-populations CD4+ and ratio of CD4+:CD8+ were also significantly (P < 0.05) higher in MOS group. The serum IgG levels were similar (P > 0.05) in both the groups. Supplementation of MOS lowered (P < 0.05) serum total- and LDL- cholesterol levels, when compared with the control group. The erythrocytic antioxidant indices were similar (P > 0.05) between the two groups. Conclusions: The results indicated that supplementation of MOS at the rate of 15 g/kg in the diet of dog augmented the cell-mediated immune response and serum lipid profile without any influences on digestibility of nutrients, hindgut fermentation and antioxidants indices.

참고문헌 (39)

  1. Roberfroid MB, "Prebiotics: the concept revisited", J Nutr, 137, pp.830S~837S (2007)
  2. Parks CW, "The effect of mannanoligosaccharides, bambermycins and virginiamycin on performance of large white male market turkeys", Poult Sci, 80, pp.718~723 (2001)
  3. Attia YA, "Performance, blood profile, carcass and meat traits and tissue morphology in growing rabbits fed mannanoligosaccharides and zinc-bacitracin continuously or intermittently", Anim Sci Pap Rep, 33, pp.85~101 (2015)
  4. Grieshop C, "Gastrointestinal and immunological responses of senior dogs to chicory and mannanoligosaccharides", Arch Anim Nutr, 58, pp.483~494 (2004)
  5. Cotter PF, "Nutritional biotechnology in the feed and food industries", pp.21~28 (2002), Nottingham University Press
  6. O’Carra R, "An assessment of the potential of mannanoligosaccharides as immunostimulants", MS, pp.1997
  7. Swanson KS, "Supplemental fructooligosaccharides and mannanoligosacchrides influence immune function, ileal and total tract nutrient digestibilities, microbial population and concentrations of protein catabolites in the large bowel of dogs", J Nutr, 132, pp.980~989 (2002)
  8. Swanson KS, "Effects of supplemental fructooligosaccharides plus mannanoligosaccharides on immune function and ileal and fecal microbial populations in adult dogs", Arch Anim Nutr, 56, pp.309~318 (2002)
  9. Pereira DIA, "Effects of consumption of probiotics and prebiotics on serum lipid levels in human", Crit Rev Biochem Mol Biol, 37, pp.259~281 (2002)
  10. Diez M, "Influence of a blend of fructooligosaccharides on nutrient digestibility and plasma metabolite concentrations in healthy beagles", Am J Vet Res, 58, pp.1238~1242 (1997)
  11. Sies H, "Antioxidant activity in cells and organs", Am Rev Respir Dis, 136, pp.478~480 (1987)
  12. NRC, "Nutrient requirements of dogs and cats" (2006), National Academy Press
  13. Samal L, "Jerusalem artichoke as a potential prebiotic : influence on nutrient utilization, hindgut fermentation and immune response of Labrador dogs", Anim Nutr Feed Techn, 12, pp.343~352 (2012)
  14. Kore KB, "Evaluation of alternative cereal sources in dog diets effect on nutrient utilisation and hindgut fermentation characteristics", J Sci Food Agr, 89, pp.2174~2180 (2009)
  15. AOAC, "Official methods of analysis" (1995), AOAC international
  16. Chaney AL, "Modified reagents for determination of urea and ammonia", Clin Chem, 8, pp.130~132 (1962)
  17. Baker SB, "The colorimetric determination of lactic acid in biological material", J Biol Chem, 138, pp.535~554 (1941)
  18. Agarwal N, "Effect of peppermint(Mentha piperita)oil on in vitro methanogenesis and fermentation of feed with buffalo rumen liquor", Anim Feed Sci Technol, 148, pp.321~327 (2009)
  19. Kim HW, "Dietary lutein stimulates immune response in the canine", Vet Immunol Immunopathol, 74, pp.315~327 (2000)
  20. Samal L, "Prebiotic potential of Jerusalem artichoke(Helianthus tuberosus)in Wistar rats : effects of levels of supplementation on hindgut fermentation, intestinal morphology, blood metabolites and immune response", J Sci Food Agr, 95, pp.1689~1696 (2015)
  21. Singh VK, "Effect of dietary energy intake on erythrocytic antioxidant defence in growing lambs fed a wheat straw based diet", Anim Prod Sci, 51, pp.642~649 (2011)
  22. Snedecor GW, "Statistical methods" (1994), Iowa State University Press
  23. Flickinger EA, "Nutrient digestibilities, microbial populations, and protein catabolites as affected by fructan supplementation of dog diets", J Anim Sci, 81, pp.2008~2018 (2003)
  24. Propst EL, "A dose–response experiment evaluating the effects of oligofructose and inulin on nutrient digestibility, stool quality, and fecal protein catabolites in healthy adult dogs", J Anim Sci, 81, pp.3057~3066 (2003)
  25. Felssner KS, "Dietetic combination of mannan-oligosaccharides and fructooligosaccharides modifies nitrogen metabolism in dogs", Semin Cienc Agrar, 37, pp.3335~3348 (2016)
  26. Diez M, "The influence of sugar-beet fibre, guar gum and inulin on nutrient digestibility, water consumption and plasma metabolites in healthy beagle dogs", Res Vet Sci, 64, pp.91~96 (1998)
  27. Flickinger EA, "Glucose-based oligosaccharides exhibit different in vitro fermentation patterns and affect in vivo apparent nutrient digestibility and microbial populations in dogs", J Nutr, 130, pp.1267~1273 (2000)
  28. Yang Y, "Effects of dietary mannanoligosaccharide on growth performance, nutrient digestibility and gut development of broilers given different cereal-based diets", J Anim Physiol Anim Nutr, 92, pp.650~659 (2008)
  29. Juskiewicz J, "Effect of adding mannan-oligosaccharide to the diet on the performance, weight of digestive tract segments, and caecal digesta parameters in young turkeys", J Anim Feed Sci, 12, pp.133~142 (2003)
  30. von Englehardt W, "Absorption of short-chain fatty acids and their role in the hindgut of monogastric animals", Anim Feed Sci Technol, 23, pp.43~53 (1989)
  31. Young M, "Characterization of oligosaccharides from an antigenic mannan of Saccharomyces cerevisiae", Glycoconj J, 15, pp.815~822 (1998)
  32. Shashidhara RG, "Effect of dietary mannanoligosaccharide on broiler breeder production traits and immunity", Poult Sci, 82, pp.1319~1325 (2003)
  33. Alarca LG, "Dietary lutein supplementation on diet digestibility and blood parameters of dog", Cienc Rural, 46, pp.2195~2201 (2016)
  34. Odiere MR, "Maternal protein deficiency during a gastrointestinal nematode infection alters developmental profile of lymphocyte populations and selected cytokines in neonatal mice", J Nutr, 143, pp.100~107 (2013)
  35. Bloom BR, "Stigma variations : observations on suppressor T cells and leprosy", Annu Rev Immunol, 10, pp.453~488 (1992)
  36. Field CJ, "The fermentable fibre content of the diet alters the function and composition of canine gut associated lymphoid tissue", Vet Immunol Immunopathol, 72, pp.325~341 (1999)
  37. Krizkova L, "Antioxidative and antimutagenic activity of yeast cell wall mannansin vitro", Mutat Res, 497, pp.213~222 (2001)
  38. Ognik K, "Dietary supplementation of mannanoligosaccharides to turkey hens on their growth performance and antioxidant status in blood", S Afr J Anim Sci, 42, pp.379~388 (2012)
  39. Pereira DI, "Effects of consumption of probiotics and prebiotics on serum lipid levels in humans", Crit Rev Biochem Mol, 7, pp.259~281 (2002)

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