섬유아세포의 생존에 대한 베바시주맙과 마이토마이신 C의 효과 비교
Comparison of the Effects Between Bevacizumab and Mitomycin C on the Survival of Fibroblasts
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목적: 섬유아세포의 생존에 대한 베바시주맙의 효과를 마이토마이신 C와 비교해보고, 일산화질소의 생성과의 관련성을 알아보고자 하 였다. 대상과 방법: 일차배양한 섬유아세포에 0, 25, 50, 100, 200 μg/mL의 베바시주맙과 마이토마이신 C에 각각 노출시킨 후 5일간 정치배 양한 다음 MTT assay와 Griess assay를 이용하여 세포의 생존과 일산화질소의 생성을 각각 측정하였다. 결과: 베바시주맙은 200 μg/mL의 고농도에서만 섬유아세포의 생존을 유의하게 저하시켰으며, 마이토마이신 C에 비해 세포증식억제효 과가 적게 나타났다. 또한 베바시주맙은 일산화질소의 생성에도 유의한 영향을 미치지 않았다. 결론: 섬유아세포에 대한 베바시주맙의 항증식효과는 마이토마이신 C에 비해 유의하게 낮았다.
Abstract
Purpose: To compare the antiproliferative effects between bevacizumab, a monoclonal antibody against vascular endothelial growth factor (VEGF), and mitomycin C in human Tenon’s capsule fibroblasts. Methods: Primarily cultured human Tenon’s capsule fibroblasts were exposed to 0, 25, 50, 100, and 200 μg/ml bevacizumab and mitomycin C, and incubated for 5 days. Cellular survival and production of nitric oxide were assessed by MTT assay and Griess assay, respectively. Results: Bevacizumab showed antiproliferative effects only at high concentrations (200 μg/ml) and revealed much less effect on the cellular survival compared to mitomycin C. In addition, bevacizumab did not affect the production of nitric oxide. Conclusions: The antiproliferative effect of bevacizumab is much lower than mitomycin C in human Tenon’s capsule fibroblasts.
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