섬유주세포에서 Dipyridamole이 활성산소종과 산화스트레스에 미치는 영향
Effect of Dipyridamole on the Reactive Oxygen Species and Oxidative Stress in Trabecular Meshwork Cells
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목적: 배양된 섬유주세포에서 dipyridamole (DPD)이 활성산소종의 생성과 산화스트레스에 미치는 영향을 알아보고자 하였다. 대상과 방법: DPD의 항산화능은 DPPH assay로 조사하였다. 인체의 섬유주세포를 일차배양한 후 0, 20, 50 μm DPD에 노출시켜DCFHDA assay로 활성산소종의 생성에 미치는 영향을 조사하였으며, t-butyhlhydroperoxide (tBHP)로 유발한 산화스트레스에 대해세포활성도에 미치는 영향을 resazurin assay로 조사하였다. 결과: DPD은 유의한 항산화능을 나타내었다(p＜0.05). 인체의 섬유주세포에서 DPD은 유의하게 활성산소종의 생성을 감소시켰으며(p ＜0.05), tBHP로 유도된 산화스트레스에 대해 DPD는 유의하게 세포 활성도를 증가시켰다(p＜0.05). DPD은 일산화질소의 생성에는유의한 영향을 미치지 않았다. 결론: DPD는 섬유주세포에서 항산화능력과 세포보호작용을 나타낼 수 있을 것으로 생각한다.
Abstract
Purpose: To investigate the effects of dipyridamole (DPD) on the production of reactive oxygen species (ROS) and oxidative stress in cultured human trabecular meshwork cells (HTMC). Methods: Antioxidant activity of DPD was determined by DPPH assay. Primarily cultured HTMC were exposed to 0, 20,and 50 μm DPD using serum-deprived media. The effect of DPD on the production of ROS was assessed with the DCHFDA assay. The effect of DPD on the t-butyl hydroperoxide (tBHP)-induced oxidative stress was assessed with resazurin assay. Results: DPD showed significant antioxidant activity. DPD significantly decreased the production of ROS (p < 0.05) and improved cellular activity significantly after treatment with t-BHP (p < 0.05). DPD did not affect the generation of nitric oxides. Conclusions: DPD suppressed the formation of ROS and possessed cytoprotective activity against the oxidative stress in HTMC.
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